Title:
IMMUNOFLUORESCENCE KIT FOR DETECTING E-CADHERIN EXPRESSION OF PERIPHERAL BLOOD CIRCULATING TUMOR CELLS OF PATIENT WITH PANCREATIC CANCER
Document Type and Number:
WIPO Patent Application WO/2022/001826
Kind Code:
A1
Abstract:
An immunofluorescence kit for detecting E-Cadherin expression of peripheral blood circulating tumor cells of a patient with pancreatic cancer, which falls within the field of molecular biology technology. Also disclosed is a kit for detecting E-Cadherin expression of peripheral blood circulating tumor cells from a patient with pancreatic cancer and a detection method. A membrane filter device is used to separate and obtain CTCs from peripheral blood of a patient with advanced or recurrent pancreatic cancer from whom a tissue sample cannot be obtained, and immunofluorescence technology is further applied to detect the E‑Cadherin expression of the CTCs. The detection method can detect the E‑Cadherin expression condition of a patient with advanced or recurrent pancreatic cancer without using a needle biopsy to obtain a tissue sample. The sampling process is a minimally invasive technology, which can realize real-time detection, can avoid false positive results caused by edge effects possibly generated during the staining process, has a good stability, reduces cell loss, and improves the accuracy of detection.
Inventors:
HAO JIHUI (CN)
LI SHENG (CN)
REN HE (CN)
LI SHENG (CN)
REN HE (CN)
Application Number:
PCT/CN2021/102099
Publication Date:
January 06, 2022
Filing Date:
June 24, 2021
Export Citation:
Assignee:
TIANJIN MEDICAL UNIV CANCER INSTITUTE & HOSPITAL (CN)
International Classes:
G01N33/58; B01D29/05; G01N33/569; G01N33/574
Foreign References:
CN111638354A | 2020-09-08 | |||
CN105510600A | 2016-04-20 | |||
CN105606823A | 2016-05-25 | |||
CN105606824A | 2016-05-25 | |||
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US20190192739A1 | 2019-06-27 | |||
CN107602691A | 2018-01-19 | |||
CN107142310A | 2017-09-08 | |||
CN106701762A | 2017-05-24 |
Attorney, Agent or Firm:
JINAN SPRING PATENT & TRADEMARK OFFICE (CN)
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