Title:
IMMUNOGENIC MENINGOCOCCAL LPS AND OUTER MEMBRANE VESICLES AND VACCINE THEREFROM
Document Type and Number:
WIPO Patent Application WO/1994/008021
Kind Code:
A1
Abstract:
The invention is directed at an immunity providing B cell activating molecule derived from a meningococcal lipopolysaccharide (LPS) having at least one epitope, said molecule comprising at least the communal part of the oligosaccharide part (core region) of lipopolysaccharides specific for at least two meningococcal immunotypes, preferably immunotypes L2 and L3 and wherein galactose is absent in the B cell activating part, as well as derivatives of the molecule with immune reaction inducing capacity. The invention is also directed at an outer membrane vesicle provided with a group of polypeptides having at least the immunoactivity of outer membrane proteins (OMP's) bound to a membrane, a polypeptide from the group of said outer membrane vesicles being a membrane anchored OMP or OMP fragment with a mutation in one of the surface loops, preferably in a 2, 3, 5, 6, 7 or 8-loop of a class I OMP. Furthermore, the invention is directed at a vaccine comprising such an outer membrane vesicle and/or lipopolysaccharide, as well as at methods for preparing a lipopolysaccharide and an outer membrane vesicle as described above.
Inventors:
VAN DER LEY PETER ANDRE (NL)
POOLMAN JAN THEUNIS (NL)
HOOGERHOUT PETER (NL)
POOLMAN JAN THEUNIS (NL)
HOOGERHOUT PETER (NL)
Application Number:
PCT/NL1993/000163
Publication Date:
April 14, 1994
Filing Date:
July 30, 1993
Export Citation:
Assignee:
NEDERLANDEN STAAT (NL)
LEY PETER ANDRE V D (NL)
POOLMAN JAN THEUNIS (NL)
HOOGERHOUT PETER (NL)
LEY PETER ANDRE V D (NL)
POOLMAN JAN THEUNIS (NL)
HOOGERHOUT PETER (NL)
International Classes:
C12N15/09; A61K39/095; A61P31/04; C07K9/00; C07K14/195; C07K14/22; C08B37/00; C12N1/21; C12N15/31; C12N15/61; C12P19/04; A61K39/00; C12R1/36; C12N; (IPC1-7): C12N15/61; A61K39/095; C08B37/00; C12P19/04; C07K9/00; C12N15/31; C12N1/21; C07K15/00
Domestic Patent References:
| WO1990006696A2 | 1990-06-28 |
Foreign References:
| NL9101359A | 1993-03-01 |
Other References:
E. WIERTZ ET AL: "T cell recognition of Neisseria meningitidis class 1 outer membrane proteins", JOURNAL OF IMMUNOLOGY., vol. 147, no. 6, September 1991 (1991-09-01), BALTIMORE US, pages 2012 - 2018
A. VERHEUL ET AL: "Minimal oligosaccharide structures required for induction of immune responses against meningococcal L1,L2 and L3,7,9 lipopolysaccharides..", INFECTION AND IMMUNITY., vol. 59, no. 10, October 1991 (1991-10-01), WASHINGTON US, pages 3566 - 3573
A. VERHEUL ET AL: "Preparation, characterization, and immunogenicity of meningococcal immunotype L2 and L3,7,9 phosphoetanolamine group-containg oligosaccahride-protein conjugates", INFECTION AND IMMUNITY., vol. 59, no. 3, March 1991 (1991-03-01), WASHINGTON US, pages 843 - 851
M. FROSCH ET AL: "Molecular characterization and expression in Escherichia coli of the gene complex encoding the polysaccharide capsule of Neisseria meningitidis group B", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA., vol. 86, March 1989 (1989-03-01), WASHINGTON US, pages 1669 - 1673
G. BOONS ET AL303: "Preparation of a well-defined sugar-peptide conjugate", BIOORGANIC AND MEDICAL CHEMISTRY LETTERS, vol. 1, no. 6, 1991, pages 303 - 308
C. FRASCH: "Vaccines for prevention of meningococcal disease", CLINICAL MICROBIOLOGY REVIEWS, vol. 2, no. SUPP, April 1989 (1989-04-01), pages S134 - S138
A. VERHEUL ET AL: "Minimal oligosaccharide structures required for induction of immune responses against meningococcal L1,L2 and L3,7,9 lipopolysaccharides..", INFECTION AND IMMUNITY., vol. 59, no. 10, October 1991 (1991-10-01), WASHINGTON US, pages 3566 - 3573
A. VERHEUL ET AL: "Preparation, characterization, and immunogenicity of meningococcal immunotype L2 and L3,7,9 phosphoetanolamine group-containg oligosaccahride-protein conjugates", INFECTION AND IMMUNITY., vol. 59, no. 3, March 1991 (1991-03-01), WASHINGTON US, pages 843 - 851
M. FROSCH ET AL: "Molecular characterization and expression in Escherichia coli of the gene complex encoding the polysaccharide capsule of Neisseria meningitidis group B", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA., vol. 86, March 1989 (1989-03-01), WASHINGTON US, pages 1669 - 1673
G. BOONS ET AL303: "Preparation of a well-defined sugar-peptide conjugate", BIOORGANIC AND MEDICAL CHEMISTRY LETTERS, vol. 1, no. 6, 1991, pages 303 - 308
C. FRASCH: "Vaccines for prevention of meningococcal disease", CLINICAL MICROBIOLOGY REVIEWS, vol. 2, no. SUPP, April 1989 (1989-04-01), pages S134 - S138
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