Title:
IMMUNOGLOBULIN-BINDING POLYPEPTIDE
Document Type and Number:
WIPO Patent Application WO/2017/069158
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a polypeptide that has a high binding capacity to immunoglobulin kappa chains and excellent alkali stability by modifying an amino acid sequence in immunoglobulin-binding domains of protein L derived from Peptostreptococcus magnus. A polypeptide having a high binding capacity to immunoglobulin kappa chains and excellent alkali stability is obtained by substituting a specific lysine residue with a basic amino acid or an amino acid having a hydroxyl group in immunoglobulin-binding domains of protein L derived from Peptostreptococcus magnus strain 3316.
Inventors:
MAJIMA EIJI (JP)
SHIMA ATSUSHI (JP)
SHIMA ATSUSHI (JP)
Application Number:
PCT/JP2016/080977
Publication Date:
April 27, 2017
Filing Date:
October 19, 2016
Export Citation:
Assignee:
PROTENOVA CO LTD (JP)
International Classes:
C12N15/09; C07K14/315; C07K17/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12P21/02
Domestic Patent References:
| WO2000015803A1 | 2000-03-23 | |||
| WO2016096644A1 | 2016-06-23 |
Foreign References:
| JP2015501299A | 2015-01-15 | |||
| JPH07507925A | 1995-09-07 | |||
| JP2016079149A | 2016-05-16 |
Other References:
PROTEIN EXPRESS CO., LTD.: "Tosha no Kumikae Tanpaku Hatsugen Gijutsu ni yori Kaihatsu·Seizo sareta Kokino Ligand o Mochiita Kotai Seisei Resin no Goshokai", INNOVATION JAPAN 2015, 27 August 2015 (2015-08-27), pages 1 - 3, XP055527464, Retrieved from the Internet
MINAKUCHI, KAZUNOBU ET AL.: "Remarkable alkaline stability of an engineered protein A as immunoglobulin affinity ligand: C domain having only one amino acid substitution", PROTEIN SCIENCE, vol. 22, 2013, pages 1230 - 1238, XP055347095
See also references of EP 3375873A4
MINAKUCHI, KAZUNOBU ET AL.: "Remarkable alkaline stability of an engineered protein A as immunoglobulin affinity ligand: C domain having only one amino acid substitution", PROTEIN SCIENCE, vol. 22, 2013, pages 1230 - 1238, XP055347095
See also references of EP 3375873A4
Attorney, Agent or Firm:
TANAKA, Junya et al. (JP)
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