I U ORECEPTOR MODULATION FOR TREATING CANCER AND VIRAL

INFECTIONS TECHNICAL FIELD THIS INVENTION relates to the immunoreceptor CD96. More particularly, this invention relates to inhibition of CD96 to thereby enhance the ability of the immune system to target tumours and other diseases or conditions that can evade the immune system.

BACKGROUND

The progression of a productive immune response requires that a number of immunological checkpoints be passed. Passage may require the presence of excitatory co-stimulatory signals or the avoidance of negative or co-inhibitory signals, which act to dampen or terminate immune activity. The immunoglobulin superfamily occupies a central importance in this coordination of immune responses, and the CD28/cytotoxic T-lymphocyte antigen-4 (CTLA-4):B7.1 B7.2 receptor ligand grouping represents the archetypal example of these immune regulators. In part the role of these checkpoints is to guard against the possibility of unwanted and harmful self-directed activities. While this is a necessary function, aiding in the prevention of autoimmunity, it may act as a barrier to successful immunotherapies aimed at targeting malignant self-cells that largely display the same array of surface molecules as the cells from which they derive. Therapies aimed at overcoming these mechanisms of peripheral tolerance, in particular by blocking the inhibitory checkpoints on T cells, offer the potential to generate antitumor activity, either as monotherapies or in synergism with other therapies that directly or indirectly enhance presentation of tumor epitopes to the immune system. Such anti-T cell checkpoint antibodies are showing promise in early clinical trials of advanced human cancers.

Furthermore, natural killer (NK) cells are innate lymphocytes critical to limit early tumor growth and metastasis NK cell functions are also regulated by the integration of signals transmitted by a wide range of activating and inhibitory receptors ² . For example, the recognition of pathogen-derived or stress-induced ligands by activating receptors such as NCRs, NKG2D, or DNAM-1 stimulate NK cells cytotoxicity and the secretion of pro-inflammatory mediators such as interferon gamma (IFN-y) ³ . In contrast, inhibitory receptors protect target cells from NK cell- ΐ

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mediated killing ⁴ . These receptors mostly recognize MHC class I and MHC class I- related molecules and include the KIR (killer cell immunoglobulin-like receptors) and LIR (leukocyte immunoglobulin-like receptors) families, the Ly49 family in mice and the CD94/NKG2 heterodimers in both species.

An emerging group of immunoglobulin superfamily members that interact with ligands of the nectin and nectin-like (necl) family has recently been described to influence NK cell and T cell functions ⁵ . These include CD226 (DNAM-1) ⁶ , CD96 (TACTILE) ⁷ , TIGIT (T cell immunoglobulin and ITIM domain) ⁸ ' ⁹ , and CRTAM (class I restricted T cell-associated molecule) ¹⁰ . DNAM-1 and TIGIT are the most extensively studied members of this family and they share a common ligand, CD 155 (necl-5; PVR) and CD112 (nectin-2; PVRL2) ^{8 n} . TIGIT also bind an additional ligand CD113 (PVRL3) ⁸ . The functions of DNAM-1 and TIGIT on NK cells are reportedly counter-balancing . In vitro, DNAM-1 potentiates the cytotoxicity of NK cells against a wide range of tumor cells ^13,1 and is critical for tumor immunosurveillance in vivo ¹³ · ¹⁵ · ¹⁶ . in contrast, TIGIT bear an ITIM motif and has been proposed prevent self-tissue damage similar to inhibitory Ly49 or KIR interactions with MHC class I ¹⁷ . Indeed, engagement of TIGIT by CD 155 has been shown to limit IFNy production and cytotoxicity by NK cells in vitro ^18,19 . However, the role of TIGIT in NK cell biology relative to the other nectin receptors DNAM-1 and CD96 remains to be assessed in vivo.

Despite being cloned 20 years ago ⁷ , little is known about CD96, the other Ig family member that shares CD155 ligand with DNAM-1 and TIGIT ^{20 i} . In humans, CD96 expression is largely confined to NK cells, CD8 T cells, and CD4 T cells ⁷ . The major ligand of CD96 is CD 155, but CD96 has also been reported to associate with CD111 (nectin-1) and play a role in promoting NK and T cell adhesion ^21:12 .

SUMMARY

Surprisingly, the present inventors have discovered that CD96 acts as a negative regulator of T cell and NK cell anti-tumor functions. Accordingly, the invention is broadly directed to use of agents that at least partly block or inhibit CD96 to thereby reduce or relieve CD96-mediated immune inhibition to enhance or restore immune surveillance in the mammal. In certain embodiments, this may facilitate treatment of diseases or conditions responsive at least partial blocking or inhibition of CD96, such as cancers and/or viral infections.

In a first aspect, the invention provides a method of reducing or relieving immune inhibition in a mammal, said method including the step of at least partly inhibiting or reducing CD96 activity in one or more cells of the mammal to thereby relieve immune inhibition and or enhance or restore immune surveillance in the mammal.

Suitably, the step of inhibiting or reducing CD96 activity in the mammal does not include, or at least depend upon, killing of CD96-expressing cells in the mammal. Preferably, the step of inhibiting or reducing CD96 activity in the mammal includes inhibiting or reducing CD96 binding to CDl 55 and/or intracellular signaling in one or more cells of the mammal that express CD96.

In one particular embodiment, the step of inhibiting or reducing CD96 activity in the mammal includes increasing or enhancing expression, production and/or secretion of one or more cytokines or chemokines. Preferably, the cytokine is interferon γ (IFN-γ). Typically, the one or more cells of the mammal are T cells, inclusive of CD4 ⁺ and CD8 ⁺ T cells, γδΤ cells, NKT cells, and natural killer (NK) cells. ^*r

In a preferred embodiment, the method relieves immune inhibition and/or enhances or restores immune surveillance in the mammal to thereby treat or prevent cancer or cancer metastasis in the mammal.

In other embodiments, the method relieves immune inhibition and/or enhances or restores immune surveillance in the mammal to thereby treat or prevent a viral infection in the mammal.

In a second aspect, the invention provides a method of screening, designing, engineering or otherwise producing a CD96-inhibitory agent, said method including the step of determining whether a candidate molecule is capable of at least partly inhibiting or reducing CD96 activity to thereby relieve immune inhibition and/or enhance or restore immune surveillance in a mammal.

In a third aspect, the invention provides a CD96-inhibitory agent screened, designed, engineered or otherwise produced according to the method of the second aspect. Jfi

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In one embodiment, the CD96-inhibitory agent is an antibody or antibody fragment.

In one particular embodiment, the CD96-inhibitory agent is an anti-cancer agent.

In another particular embodiment, the CD96-inhibitory agent is an anti- viral agent.

In a fourth aspect, the invention provides a CD96-inhibitory agent according to the third aspect for use according to the method of the first aspect.

Suitably, according to the aforementioned aspects the mammal is a human. Unless the context requires otherwise, the terms "comprise", "comprises" and

"comprising", or similar terms are intended to mean a non-exclusive inclusion, such that a recited list of elements or features does not include those stated or listed elements solely, but may include other elements or features that are not listed or stated.

The indefinite articles and 'an' are used here to refer to or encompass singular or plural elements or features and should not be taken as meaning or defining "one" or a "single" element or feature.

BRIEF DESCRIPTION OF THE FIGURES

Figure 1: CD96 competes with DNAM-1 for CD155 binding, a, b The expression of CD96 was analyzed by flow cytometry on the indicated spleen lymphocyte populations from C57BL/6 WT (light grey) and CD96 ^J - mice (dark grey). The representative FACS Histograms (a) and the mean + SD (b) of 3 mice from one representative experiment out of 3 are shown, c, d The expression of CD96, DNAM- 1 and TIGIT was determined on WT spleen NK cells freshly isolated or activated for 48 hrs with IL-2 (1000 U/ml). e. The binding of mouse CD155-Fc coupled with AF- 647 to purified NK cells freshly isolated from WT, CD96 ^A , DNAM-1 ^A or DNAM-1 ^'A CD9 mice was assessed at the indicated concentrations by flow cytometry, f. The binding of CD155-Fc coupled with AF-647 (10 μg/ml) was analyzed on purified WT NK cells in the presence of anti-CD96 and or anti-DNAM-1 mAbs. g. The binding of DNAM-1 -Fc labeled with AF-647 (0.5-10 μg ml) at the cell surface of BMDC was analyzed in the presence of 50 g ml of control Ig, recombinant CD96 or TIGIT-Fc. c-g. The representative FACS Histograms and the mean + SD of triplicate wells from one representative experiment out of at least 3 experiments are shown. *** p<0.001 Student T test.

Figure 2: CD96 engagement by CD 155 regulate NK cell production of IFNy. CD96 binding to CD155-Fc limits the production of IFN-yby NK cells induced by exogenous cytokines (a, b, d) and NK cell receptors (c). a, b, d. We analyzed the intracellular production of IFN-γ by freshly purified CD9 , TIGIT ^1' and WT NK cells in the presence or absence of anti-CD96 (50 μ /πι1) in response to IL-12 (25- 100 pg ml) and IL-18 (50 ng/ml) using plates coated with or without CD155-Fc (0.5 μg well), c. We analyzed the intracellular production of IFN-γ by IL-2-activated NK cells from CD96 ^1' and WTmice using plates coated with anti-NKl .l (2.5 μg/ well) and CD155-Fc (0.5 μg/ well). The representative FACS Histograms (a) and the mean + SD of triplicate wells (b, c, d) from one representative experiment out of 3 are shown. *p <0.05, ** p <0.01, *** p<0.001, Student T test.

Figure 3: CD96 limits NK cell-dependent tumor immunosurveillance. a, b. CD96 and DNAM-1 have an opposite role in the control of B16F10 metastasis, a. 2 x 10 ⁵ B16F10 cells were intravenously injected into WT, CD9& , DNAM-1 ^' ' ^' and DNAM-1 ^{' '} CD9 ^/' mice and metastatic burden was quantified in the lungs after 14 days. Representative experiment out of 3. b. Pictures showing the lung of WT and CD96 ^"A mice two weeks after the injection of 2 x 10 ^s and 5 x 10 ^s B16F10 cells. Representative experiment out of two. c. CD96 and TIGIT compete with DNAM-1 for the binding of CD155 at the cell surface of B16F10. The binding of DNAM-1 -Fc labeled with AF-647 (0.5-20 μg ml) at the cell surface of B16F10 cells was analyzed in the presence of 50 g ml of control Ig, recombinant CD96 or TIGIT-Fc. The FACS histograms and the mean + SD of triplicate wells from one representative experiment out of 3 are shown, d. A 4 hr ^5l Cr release assay was performed between B16F10 cells and IL-2-activated NK cells from WT, DNAM-1 ^1' and CD9G ^1' mice at the indicated effector target ratios. Solid circles represent WT NK cells, open circles represent CD96 ^"7" NK cells and solid squares represents DNAM-1 ^'/_ NK cells, e-h. CD96 and DNAM-1 have an opposite role in the immunosurveillance of MCA induced fibrosarcoma mediated by NK cells, e-h Groups of 15-30 male, WT, DNAM-1 ^' ' ^' and CD9G ^1' and DNAM-1 ^'1' CD9S ¹ mice were injected with MCA (100 μg/mouse). The survival (e-g) and the growth curves of individual mice with sarcoma (h) are shown, f. WT mice were treated with an anti-CD96, anti-DNAM-1 or anti-CD155 mAbs as defined in the Materials and Methods, g. WT and CD9& ^A mice were injected with 100 μg MCA and treated with either a control antibody, anti- IFN-γ antibody, or anti-asialoGMl . * p<0.05 Mantel-Cox test.

Figure 4: Anti-CD96 mAb has single agent activity and enhances the anti-tumor responses of anti-PDl. C57BL/6 wild type (WT) mice were injected subcutaneously with AT3-OVA ^dim tumor cells (10 ⁶ cells) and treated on day 16, 20 and 24 with intraperitoneal injections of anti-CD96 mAb (3.3, 250 μg i.p) or anti-PD-1 (RMP1- 14, 250 μg i.p.). Means ± SEM of 5 mice per group (mm ² ) are shown (*: p<0.05 compared to clg alone by Mann- Whitney test).

Figure 5: Anti-CD96 mAb enhances anti-tumor responses generated by Doxorubicin (DOX) chemotherapy. C57BL/6 wild type (WT), DNAM-l ^"7' , and CD96 ^" ' ^" mice were injected subcutaneously with AT3-OVA ^d,m tumor cells (10 ⁶ cells) and treated on day 14 with control PBS or DOX (50 microliters, 2 mM, intratumor). Some groups of WT mice also received on day 12, 14, 18, 21, 24 and 28 intraperitoneal injections of anti-CD96 mAb (3.3, 250 μg i.p) or anti-DNAM-1 (480.1 , 250 g i.p.). Means ± SEM of 5 mice per group (mm ² ) are shown.

Figure 6: Enhanced anti-tumor responses of Doxorubicin (DOX) chemotherapy with host CD96 deficiency. C57BL/6 wild type (WT), DNAM-l ^" ' ^" , and CD96 ^"A mice were injected subcutaneously with AT3-OVA ^dim tumor cells (10 ⁶ cells) and treated on day 16 with control PBS or DOX (50 microliters, 2 mM, intratumor). Means ± standard errors of 5 mice per group (mm ² ) are shown.

Figure 7: Anti-CD96 mAb enhances anti-tumor responses generated by Doxorubicin (DOX) chemotherapy. C57BL/6 wild type (WT) mice were injected subcutaneously with AT3-OVA ^d,m tumor cells (10 ⁶ cells) and treated on day 16 with control PBS or DOX (50 microliters, 2 mM, intratumor). Some groups of WT mice also received on day 16, 20, and 23 intraperitoneal injections of anti-CD96 mAb (3.3, 250 μg i.p). Means ± SEM of 5 mice per group (mm ² ) are shown (*: p<0.05 compared to clg alone by Mann- Whitney test).

Figure 8: Early anti-CD96 mAb enhances anti-tumor responses generated by anti-PD-1 and anti-CTLA-4 mAbs. C57BIJ6 wild-type (WT) mice were injected subcutaneously with B16-OVA melanoma cells (10 ⁵ cells) and treated on day 1, 5, and 9 with intraperitoneal injections of anti-CD96 mAb (3.3, 250 μ i.p), anti-PD-1 mAb (RMP1-14, 250 μg i.p.), anti-CTLA-4 (UC10-4F10, 250 i.p.), anti- CD96/anti-PD-l mAbs (250 μg i.p each), anti-CD96/anti-CTLA-4 mAbs (250 g i.p each) or control Ig (cig) (2A3, 250 μg i.p). Means ± SEM of 5 mice per group (mm ² ) are shown (*: p<0.05 compared with anti-CD96 alone, by Mann- Whitney test). Figure 9: Late anti-CD96 mAb enhances anti-tumor responses generated by anti-PD-1 mAb. C57BL/6 wild-type (WT) mice were injected subcutaneously with B16-OVA melanoma cells (10 ⁵ cells) and treated on day 16, 20, and 24 with intraperitoneal injections of anti-CD96 mAb (3.3, 250 μg i.p), anti-PD-1 mAb (RMP1-14, 250 μg i.p.), anti-CTLA-4 (UC10-4F10, 250 μg i.p.), anti-CD96/anti- PD-1 mAbs (250 μg i.p each), anti-CD96/anti-CTLA-4 mAbs (250 μg i.p each) or control Ig (cig) (2A3, 250 μg i.p). Means ± SEM of 5 mice per group (mm ² ) are shown (*: p<0.05 compared with anti-CD96 alone by Mann- Whitney test).

Figure 10: Host CD96 promotes B16F10 lung metastasis. C57BIJ6 wild type (WT), DNAM-l ^" ' ^" , CD96 ^" , and DNAM-1 ^{" "} CD96 ^{" "} mice were injected intravenously with B16F10 melanoma cells (10 ⁵ cells) and metastatic burden was quantified in the lungs after 14 days by counting colonies on the lung surface. Means ± SEM of 9-17 mice per group are shown (*: p<0.05 compared with WT by Mann- Whitney test). Figure 11: Host CD96 promotes RM-1 lung metastasis. C57B 176 wild type (WT), DNAM-l ^" ' ^" , CD96 ^"A , and DNAM-l ^/" CD96 ^{" "} mice were injected intravenous with RM1 prostate carcinoma cells (10 ⁴ cells) and metastatic burden was quantified in the lungs after 14 days by counting colonies on the lung surface. Means ± SEM of 10-15 mice per group are shown (*: p<0.05 compared with WT by Mann- Whitney test).

Figure 12: Host CD96 promotes 3LL lung metastasis. C57BL/6 wild type (WT), DNAM-l ^"7" , CD96 ^"7" , and DNAM-l ^" ' ^" CD96 ^" ' ^" mice were injected intravenously with 3LL lung carcinoma cells (10 ⁵ cells) and metastatic burden was quantified in the lungs after 14 days by counting colonies on the lung surface. Means ± SEM of 5 mice per group are shown (*: p<0.05 compared with WT by Mann- Whitney test). Figure 13: Anti-CD96 suppresses B16F10 lung metastasis, alone and in combination with T cell checkpoint blockade. C57BL 6 wild type (WT) mice were injected intravenous with B16F10 melanoma cells (10 ^s cells). On day 0 and 3 after tumor inoculation, mice were treated with intraperitoneal injections of anti-CD96 mAb (3.3, 250 μg i.p), anti-PD-1 mAb (RMP1-14, 250 μg i.p.), anti-CTLA-4 (UC10- 4F10, 250 μg i.p.), anti-CD96/anti-PD-l mAbs (250 μg i.p each), anti-CD96/anti- f

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CTLA-4 mAbs (250 μg i.p each) or control Ig (cig) (2A3, 250 μg i.p). Metastatic burden was quantified in the lungs after 14 days by counting colonies on the lung surface. Means ± SEM of 5 mice per group are shown (*: p<0.05 compared with anti-CD96 alone by Mann- Whitney test).

Figure 14: Anti-CD96 suppresses RM-1 lung metastasis, alone and in combination with T cell checkpoint blockade. C57BL/6 wild type (WT) mice were injected intravenous with RM-1 prostate carcinoma cells (10 ⁴ cells). On day 0 and 3 after tumor inoculation, mice were treated with intraperitoneal injections of anti- CD96 mAb (3.3, 250 ^g i.p), anti-PD-1 mAb (RMP1-14, 250 μg i.p.), anti-CTLA-4 (UC10-4F10, 250 μg i.p.), anti-CD96/anti-PD-l mAbs (250 μg i.p each), anti- CD96/anti-CTLA-4 mAbs (250 μg i.p each) or control Ig (cig) (2A3, 250 μ% i.p). Metastatic burden was quantified in the lungs after 14 days by counting colonies on the lung surface. Means ± SEM of 5 mice per group are shown (*: p<0.05 compared with anti-CD96 alone by Mann- Whitney test).

Figure 15: Late anti-CD96 mAb enhances anti-tumor responses generated by anti-PD-1 mAb. C57BL/6 wild-type (WT) mice were injected subcutaneously with MC38-OVA ^dim colon adenocarcinoma cells (10 ⁶ cells) and treated on day 14, 18, 22, and 26 with intraperitoneal injections of anti-CD96 mAb (3.3, 250 μg i.p), anti-PD-1 mAb (RMP1-14, 250 μg i.p.), anti-CTLA-4 (UC10-4F10, 250 μg i.p.), anti- CD96/anti-PD-l mAbs (250 g i.p each), anti-CD96/anti-CTLA-4 mAbs (250 μg i.p each) or control Ig (cig) (2A3, 250 μg i.p). Means ± SEM of 5 mice per group (mm ² ) are shown (*: p<0.05 compared with anti-CD96 alone by Mann- Whitney test).

DETAILED DESCRIPTION

The present invention is at least partly predicated on the unexpected discovery that CD96 is highly expressed by resting NK cells and T cell subsets and competes with DNAM-1 for the binding of CD155 on resting NK cells. Using CD96 ^"A mice, it is demonstrated that CD96 dampens or suppresses NK cell production of IFN-γ in vitro and in vivo, through competition with DNAM-1 for CD155 binding and also through a direct inhibition. Furthermore, CD96 ^{" _} mice were shown to be more resistant to 3'-methylcholanthrene (MCA)-induced tumor formation as an indicator of carcinogenesis, or B16F10 (melanoma), RM-1 (prostate cancer), 3LL (lung cancer) experimental metastasis. Based on these observations, it is proposed that CD96 normally acts as a negative regulator of T and NK cell antitumor functions, particularly although not exclusively through suppression of IFN-γ production and/or secretion. Accordingly, the invention provides methods of relieving or reducing the negative immunoregulatory function of CD96 to thereby promote or restore immune surveillance, particularly by T cells and NK cells, to thereby treat or prevent cancer, cancer cell metastasis and/or viral infections.

An aspect of the invention therefore provides a method of reducing or relieving immune inhibition in a mammal, said method including the step of at least partly inhibiting or reducing CD96 activity in one or more cells of the mammal to thereby relieve immune inhibition and/or enhance or restore immune surveillance in the mammal.

By "relieving immune inhibition" in the context of CD96 is meant at least partly eliminating, removing or overcoming a normal activity or function of CD96 in suppressing or inhibiting one or more immune functions of cells that normally express CD96. Typically, the one or more cells that normally express CD96 are T cells, inclusive of CD4 ⁺ and CD8 ⁺ T cells, γδΤ cells, NKT cells, and natural killer (NK) cells. In some embodiments, relieving immune inhibition may include or relate to abrogating peripheral tolerance to foreign pathogens, host cells displaying foreign pathogens (e.g displaying foreign pathogen-derived peptides in the context of self- MHC) and or cancerous cells or tissues of the host.

By "enhance or restore immune surveillance' ^' ' is meant at least partly improving or promoting the ability of one or more elements of the immune system to monitor, detect and/or respond to foreign pathogens, host cells displaying foreign pathogens {e.g displaying foreign pathogen-derived peptides in the context of self- MHC) and/or cancerous cells or tissues of the host. Suitably, the elements of the immune system are one or more cells that normally express CD96, such as T cells, inclusive of CD4 ⁺ and CD8 ⁺ T cells γδΤ cells, NKT cells and natural killer (NK) cells.

At least partly inhibiting or reducing CD96 activity in one or more cells of the mammal may be performed, facilitated or achieved by administration of a "CD96-inhibitory agent" to the mammal. A CD96-inhibitory agent may be any molecule that possesses or displays an ability to at least partly inhibit or reduce a biological activity of CD96. Biological activities of CD96 include one or more of binding CD 155, eliciting intracellular signaling and stimulating or inducing expression and/or secretion of cytokines and/or chemokines. Preferably, the cytokines or chemokines include any pro-inflammatory cytokine or chemokine inclusive of ΜΙΡ-Ια, ΜΙΡ-Ιβ, RANTES, TNF-a and IFN-γ, although without limitation thereto. Preferably, the cytokine is IFN-γ. Measurement of expression, production and/or secretion of one or more cytokines or chemokines, or nucleic acids encoding same, will be described in more detail hereinafter..

In one embodiment, the CD96-inhibitory agent inhibits, blocks or antagonizes a binding interaction between CD96 and CD 155. By way of example only, the CD96-inhibitory agent may bind to an extracellular domain of CD96, or a portion thereof, that is capable of interacting with CD 155 (e.g. binding CD 155 or being bound by CD155) to thereby at least partly inhibit or block CD96 binding to CD155.

In another embodiment, the CD96-inhibitory agent is a molecule that possesses or displays an ability to inhibit or reduce CD96 signaling activity. Inhibition or reduction of CD96 signaling activity may be through inhibiting, blocking or antagonizing a binding interaction with CD 155 or may be through blocking CD96-initiated signaling that would normally occur in response to CD 155 binding. By way of example, CD96 comprises an immunoreceptor tyrosine-based inhibitory motif (ITIM). ITIMs are structurally defined as 6-amino acid sequences comprising a tyrosine (Y) residue with partly conserved N-terminal (Y -2) and C- terminal (Y+3) residues. A general but non-limiting motif is (S/ /LXYXXI/V/L), wherein X is any amino acid. For example, isoform 1 of CD96 comprises the ITIM sequence IKYTCI wherein Y is residue 566.

It has been proposed that when co-aggregated with activating receptors,

ITIMs are phosphorylated by Src-family tyrosine kinases, which enables them to recruit Src homology 2 domain-containing phosphatases (PTPases) that antagonize activation signals. Accordingly, in one embodiment the CD96-inhibitory agent possesses or displays an ability to inhibit or reduce CD96 signaling activity mediated by the CD96 ITIM. Preferably, inhibition or reduction of CD96 signaling activity mediated by the CD96 ITIM enables increased or enhanced chemokine and/or cytokine (e.g IFN-γ) expression, production and/or secretion by a cell expressing CD96. The CD96-inhibitory agent may be a protein (inclusive of peptides, antibodies and antibody fragments), a nucleic acid (inclusive of inhibitory RNA molecules such as ribozymes, RNAi, miRNA and siRNA, although without limitation thereto), a lipid, a carbohydrate, a small organic molecule or any combination of these (e.g a glycoprotein, a lipoprotein, a peptide-nucleic acid etc).

In one particular embodiment, the CD96-inhibitory agent is an antibody or antibody fragment that binds CD96. In one form the antibody binds CD96 and at least partly blocks or inhibits CD96 binding to CD155.

Antibodies may be polyclonal or monoclonal, native or recombinant. Antibody fragments include Fab and Fab'2 fragments, diabodies and single chain antibody fragments (e.g. scVs), although without limitation thereto. Antibodies and antibody fragments may be modified so as to be administrable to one species having being produced in, or originating from, another species without eliciting a deleterious immune response to the "foreign" antibody. In the context of humans, this is "humanization" of the antibody produced in, or originating from, another species. Such methods are well known in the art and generally involve recombinant "grafting" of non-human antibody complementarity determining regions (CDRs) onto a human antibody scaffold or backbone.

Suitably, the step of inhibiting or reducing CD96 activity in the mammal does not include killing CD96-expressing cells in the mammal. In this context, "killing" may refer to any antibody-mediated cytotoxic mechanism such as complement- mediated cytolysis and antibody-mediated cell-mediated cytotoxicity (ADCC), the latter typically mediated by natural killer (NK) cells, macrophages, neutrophils and eosinophils. In this regard, it may be advantageous to use antibody fragments lacking an Fc portion or having a mutated Fc portion.

The step of inhibiting or reducing CD96 activity in the mammal may be achieved or facilitated by administering a CD96-inhibitory agent to the mammal.

By "administering" is meant the introduction of the CD96-inhibitory agent into the mammal by a particular route. Suitably, a therapeutically effective amount of the CD96-inhibitory agent is administered to the mammal.

The term "therapeutically effective amount" describes a quantity of a specified agent sufficient to achieve a desired effect in a mammal being treated with that agent. JH

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Generally, the method of the invention may be useful in reducing or relieving CD96-mediated immune inhibition, suppression or peripheral tolerance. Suitably, the method facilitates treatment or prevention of one or more diseases or conditions that are responsive to at least partly blocking CD96-mediated immune inhibition, suppression or peripheral tolerance.

As used herein, "treating ^* or "treat" or "treatment refers to a therapeutic intervention that at least party eliminates or ameliorates one or more existing or previously identified symptoms of a disease or condition that is responsive to at least partly blocking CD96-mediated immune inhibition, suppression or peripheral tolerance.

As used herein, "preventing ^' ' or "prevent" or "prevention" refers to a prophylactic treatment initiated prior to the onset of a symptom of a disease or condition that is responsive to at least partly blocking CD96-mediated immune inhibition, suppression or peripheral tolerance, so as to at least partly or temporarily prevent the occurrence of the symptom.

Typically, the disease or condition that is responsive to at least partly blocking CD96-mediated immune inhibition, suppression or peripheral tolerance is any disease or condition where enhanced or restored immune surveillance can benefit a subject suffering from the disease or condition. Such diseases and conditions may include those where persistence of the disease or condition can be controlled or suppressed by cell-mediated immunity. Non-limiting examples include cancers and viral infections. Particular viral infections contemplated by the invention include persistent viral infections such as caused by human immunodeficiency virus (HIV), Epstein Barr Virus (EBV), Herpes Simplex Virus (HSV inclusive of HSV1 and HSV2), Human Papillomavirus (HPV), Varicella zoster virus (VSV) and Cytomegalovirus (CMV), although without limitation thereto.

In a preferred embodiment, the method reduces or relieves immune inhibition in a mammal sufficient to treat or prevent cancer or cancer metastasis in the mammal. Suitably, the cancer may be any that is responsive to at least partly blocking CD96-mediated immune inhibition, suppression or peripheral tolerance. Cancers may be in the form of solid tumors, sarcomas, lymphomas, myelomas, carcinomas, melanomas, cytomas and meningiomas, although without limitation thereto. Non-limiting examples of cancers include cancers of the adrenal gland, bladder,, bone, bone marrow, brain, breast, cervix, gall bladder, ganglia, gastrointestinal tract, heart, kidney, liver, lung, muscle, ovary, pancreas, pituitary, parathyroid, prostate, salivary glands, skin, spleen, testis, thyroid, and uterus. Particular non-limiting examples of cancers include colon cancer, lung cancer and prostate cancer. In some embodiments, the cancer is a metastatic cancer, which is capable of migrating to another site, tissue or organ in the body and forming a tumor at that site, tissue or organ. This may occur repeatedly over time. A particularly aggressive metastatic cancer contemplated by the invention is metastatic melanoma.

It will also be appreciated that the method of treatment or prevention of cancer may further include co-administration of one or more other therapeutic agents that facilitate cancer treatment or prevention. By way of example only, these include: chemotherapeutic agents such as paclitaxel, doxorubicin, methotrexate and cisplatin, although without limitation thereto; and/or biotherapeutic agents such as anti-PD-1 antibodies (e.g. Nivolumab) and anti-CTLA4 antibodies (e.g Ipilimumab), although without limitation thereto. Also contemplated are bi-specific antibodies that bind both CD96 and one or more other molecules including but not limited to PD-1 and CTLA4.

The one or more other agents that facilitate cancer treatment or prevention may be administered in combination with the CD96-inhibitory agent or be administered separately, as is well understood in the art.

In some embodiments, the CD96-inhibitory agent may be formulated alone or together with the one or more other agents is in the form of a pharmaceutical composition.

Suitable dosages of CD96-inhibitory agents, alone or together with other therapeutic agents, for mammalian administration, including human administration, may be readily determined by persons skilled in the art.

Suitably, the pharmaceutical composition comprises an appropriate pharmaceutically-acceptable carrier, diluent or excipient.

Preferably, the pharmaceutically-acceptable carrier, diluent or excipient is suitable for administration to mammals, and more preferably, to humans.

By "pharmaceutically-acceptable carrier, diluent or excipient" is meant a solid or liquid filler, diluent or encapsulating substance that may be safely used in systemic administration. Depending upon the particular route of administration, a variety of carriers, diluents and excipients well known in the art may be used. These carriers, diluents and excipients may be selected from a group including sugars, f

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starches, cellulose and its derivatives, malt, gelatine, talc, calcium sulfate, vegetable oils, synthetic oils, polyols, alginic acid, phosphate buffered solutions, emulsifiers, isotonic saline and salts such as mineral acid salts including hydrochlorides, bromides and sulfates, organic acids such as acetates, propionates and malonates, and pyrogen-free water.

A useful reference describing pharmaceutically acceptable carriers, diluents and excipients is Remington's Pharmaceutical Sciences (Mack Publishing Co. NJ USA, 1991).

Any safe route of administration may be employed for providing a subject with compositions comprising the CD96-inhibitory agent. For example, oral, rectal, parenteral, sublingual, buccal, intravenous, intra-articular, intra-muscular, intradermal, subcutaneous, inhalational, intraocular, intraperitoneal, intracerebroventricular, transdermal, and the like may be employed.

A further aspect of the invention provides a method of screening, designing, engineering or otherwise producing a CD96-inhibitory agent, said method including the step of determining whether a candidate molecule is capable of at least partly inhibiting or reducing CD96 activity to thereby relieve immune inhibition and/or enhance or restore immune surveillance in a mammal.

The invention also provides a CD96-inhibitory agent screened, designed, engineered or otherwise produced according to the aforementioned aspect.

The candidate molecule may be a protein (inclusive of peptides, antibodies and antibody fragments), a nucleic acid (inclusive of inhibitory RNA molecules such as ribozymes, RNAi, miRNA and siRNA, although without limitation thereto), a lipid, a carbohydrate, a small organic molecule or any combination of these e.g a glycoprotein, a lipoprotein, a peptide-nucleic acid etc).

In some embodiments, the candidate modulator may be rationally designed or engineered de novo based on desired or predicted structural characteristics or features that indicate the candidate modulator could block or inhibit one or more biological activities of CD96, such as CD155 binding, intracellular signaling and/or IFN-γ production and/or secretion. In other embodiments, the candidate modulator may be identified by screening a library of molecules without initial selection based on desired or predicted structural characteristics or features that indicate the candidate modulator could block or inhibit one or more biological activities of t

15

CD96. Such libraries may comprise randomly generated or directed libraries of proteins, peptides, nucleic acids, recombinant antibodies or antibody fragments (e.g. phage display libraries), carbohydrates and/or lipids, libraries of naturally-occurring molecules and/or combinatorial libraries of synthetic organic molecules.

Non-limiting examples of techniques applicable to the design and/or screening of candidate modulators may employ X-ray crystallography, NMR spectroscopy, computer assisted screening of structural databases, computer-assisted modelling or biochemical or biophysical techniques which detect molecular binding interactions, as are well known in the art.

Biophysical and biochemical techniques which identify molecular interactions include competitive radioligand binding assays, co-immunoprecipitation, fluorescence-based assays including fluorescence resonance energy transfer (FRET) binding assays, electrophysiology, analytical ultracentrifugation, label transfer, chemical cross-linking, mass spectroscopy, microcalorimetry, surface plasmon resonance and optical biosensor-based methods, such as provided in Chapter 20 of CURRENT PROTOCOLS IN PROTEIN SCIENCE Eds. Coligan et al. , (John Wiley & Sons, 1997) Biochemical techniques such as two-hybrid and phage display screening methods are provided in Chapter 19 of CURRENT PROTOCOLS IN PROTEIN SCIENCE Eds. Coligan et al, (John Wiley & Sons, 1997).

Accordingly, an initial step of the method may include identifying a plurality of candidate molecules that are selected according to broad structural and/or functional attributes, such as an ability to bind CD96.

The method may include a further step that measures or detects a change in one or more biological activities of CD96 in response to the candidate molecule(s). These may include CD 155 binding, intracellular signaling, cytokine and/or chemokine production or secretion and/or protection from tumor challenge in an in vivo model.

Inhibition of CD155 binding to CD96 by a candidate molecule may be determined by any of several techniques known in the art including competitive radioligand binding assays, surface plasmon resonance (e.g. BIACore™ analysis), co-immunoprecipitation and fluorescence-based analysis of the ability of a candidate inhibitor to block CD155 binding to CD96 (such as by flow cytometry where CD155 is labeled with a fluorophore). Non-limiting examples of fluorophores include fluorescein isothiocyanate (FITC), allophycocyanin (APC), fluoroscein derivatives f

16

such as FAM and ROX, Texas Red, tetramethylrhodamine isothiocyanate (TRITL), R-Phycoerythrin (RPE), Alexa and Bodipy fluorophores, although without limitation thereto.

Alternatively, this fluorescence-based analysis could include FRET analysis (e.g. one protein coupled to a donor fluorophore, the other coupled to an acceptor fluorophore), although without limitation thereto.

In some embodiments, intracellular signaling may be measured directly at the level of CD96, such as by measuring recruitment of SH2 domain-containing PTPases by CD96 expressed by NK cells, or T cell subsets. A candidate molecule of the invention suitably prevents or reduces recruitment of SH2 domain-containing PTPases by CD96 in the presence of CD 155. According to this embodiment, the candidate molecule may at least partly inhibit or prevent binding between CD96 and CD 155, thereby at least partly inhibiting or preventing intracellular signaling by CD96, and/or at least partly inhibit or prevent intracellular signaling by CD96 despite CD 155 binding.

In other embodiments, an effect of a candidate molecule on CD96 may be determined by measuring expression, production and/or secretion of one or more cytokines or chemokines by cells expressing CD96. Generally, changes in cytokine or chemokine expression production and/or secretion may be measured at the level of gene expression (such as by RT-PCR of cytokine mRNA), measurement of cytokine or chemokine protein located intracellularly (e.g by immunocytochemistry using cytokine- or chemokine-specific antibodies) and/or measurement of secreted cytokines or chemokines such as by flow cytometric Cytokine Bead Array (such as commercially available from BD Biosciences), by ELISA using cytokine- or chemokine-specific antibodies and by bioassays that use cytokine- or chemokine- responsive cell lines to measure cytokines and/or chemokines secreted into cell supernatants. The cytokine may be any pro-inflammatory cytokine or chemokine inclusive of ΜΙΡ-Ια, ΜΙΡ-Ιβ, RANTES, TNF-a and IFN-γ, although without limitation thereto. Preferably, the cytokine is IFN-γ.

Preferably, the CD96-inhibitory effect of a candidate molecule may be determined using an in vivo tumor challenge model. For example, a mouse model using CD96-expressing mice may be used to determine the ability of a candidate molecule to inhibit or prevent tumor formation and or growth in response to an administered carcinogenic agent such as methycholanthrene (MCA). In another f

17 example, a mouse model using CD96-expressing mice may be used to determine the ability of a candidate molecule to inhibit or prevent tumor formation and/or growth in response to administration of tumor cells such as melanomas, colon adenocarcinomas, prostate carcinomas and mammary carcinomas, although without limitation thereto. Other mouse models may utilize mice that are predisposed to spontaneously forming tumors including but not limited to MMTV-polyoma, MT mammary cancer, DMBA/TPA induced skin cancer, p53 loss lymphoma/sarcoma and TRAMP Tg prostate cancer.

It will be understood that the method of this aspect may be performed iteratively, whereby multiple rounds of screening, design, and biological testing are performed. This may include where a candidate molecule is structurally modified before each round, thereby enabling "fine-tuning" of the candidate molecule.

It will also be appreciated that the method may be performed in a "high throughput", "automated" or "semi-automated" manner, particularly during early stages of candidate molecule identification and selection.

In a preferred embodiment, the candidate molecule is an antibody or antibody fragment. As hereinbefore described, antibodies may be polyclonal or monoclonal, native or recombinant. Antibody fragments include Fab and Fab'2 fragments, diabodies and single chain antibody fragments (e.g. scVs), although without limitation thereto. Well-known protocols applicable to antibody production, selection, purification and use may be found, for example, in Chapter 2 of Coligan et al, CURRENT PROTOCOLS IN IMMUNOLOGY (John Wiley & Sons NY, 1991- 1994) and Harlow, E. & Lane, D. Antibodies: A Laboratory Manual, Cold Spring Harbor, Cold Spring Harbor Laboratory, 1988, which are both herein incorporated by reference.

Polyclonal antibodies may be prepared for example by injecting CD96 or a fragment (e.g a peptide) thereof into a production species, which may include mice or rabbits, to obtain polyclonal antisera. Methods of producing polyclonal antibodies are well known to those skilled in the art. Exemplary protocols that may be used are described for example in Coligan et al., CURRENT PROTOCOLS IN IMMUNOLOGY, supra, and in Harlow & Lane, 1988, supra.

Monoclonal antibodies may be produced using the standard method as for example, described in an article by Kohler & Milstein, 1975, Nature 256, 495, which is herein incorporated by reference, or by more recent modifications thereof as for r

18

example, described in Coligan et al, CURRENT PROTOCOLS IN IMMUNOLOGY, supra by immortalizing spleen or other antibody producing cells derived from a production species which has been inoculated with one or more of the isolated proteins, fragments, variants or derivatives of the invention. Suitably, the antibody or antibody fragment is suitable for administration to a human. In this context, as hereinbefore described the antibody or antibody fragment may be a "humanized" form of an antibody or antibody fragment produced in, or originating from, another species. Such methods are well known in the art and generally involve recombinant "grafting" of non-human antibody complementarity determining regions (CDRs) onto a human antibody scaffold or backbone.

In a preferred embodiment, the antibody or antibody fragment does not kill CD96-expressing cells upon administration to a human. In this context, "killing" may refer to any antibody-mediated cytotoxic mechanism such as complement- mediated cytolysis and antibody-mediated cell-mediated cytotoxicity (ADCC), the latter typically mediated by natural killer (NK) cells, macrophages, neutrophils and eosinophils. In this regard, it may be advantageous to use antibody fragments lacking an Fc portion or having a human Fc portion (e.g a humanized antibody).

A CD96-inhibitory agent screened, designed, engineered or otherwise produced according to the aforementioned aspect may be used according to the method of the first aspect (e.g as an anti-cancer agent and/or an anti-viral agent), preferably in the form of a pharmaceutical composition as hereinbefore described.

So that the invention may be readily understood and put into practical effect, reference is made to the following non-limiting examples. EXAMPLES

Example 1

CD96 binding to CD1SS and the effects of CD96 inhibition and knockout in mouse tumor models

Materials and Methods

Mice

Wild Type C57BL/6 mice were purchased from the Walter and Eliza Hall Institute for Medical Research or ARC Animal Resource Centre. C57BL/6 CD96 ^"A mice were generated by Dr. Marco Colonna and Dr. Susan Gilfillan at the r

19

Washington University School of Medicine (St Louis, MO, USA) as follows. A targeting construct designed to replace exons 1 and 2 of CD96, including the start site, with a MCl-neor gene flanked by loxP sites was electroporated into El 4.1 (129P2/01aHsd) embryonic stem cells (Fig. SI). Chimeras transmitting the targeted allele were obtained from two clones following injection into C57BL/6 blastocysts. Mice carrying the targeted allele were , bred to C57BL/6 mice expressing a Cre transgene under the CMV promoter to delete the MCl-neor gene (Schwenk et al., 1995). The CD96 deletion was backcrossed onto a C57BL/6 background, facilitated by a genome- wide screening of polymorphic microsatellite markers at 10- centiMorgan intervals at each generation. CD96 ^+/" >99% C57BL/6 mice were intercrossed to generate the CD96 ^{~ _} mice. DNAM-1 ^"A mice have already been described. DNAM-1 ^A CD96 ^'A were generated by intercrossing CD96 ^'7" with DNAM- Y ^1' mice. Mice were bred and used between the ages of 6-14 weeks. All experiments were approved by animal ethics committee.

Cell Culture

B16F10, RM-1, 3LL, AT3, MC38 and YAC-1 cell lines were grown in complete RPMI Medium (Gibco, Invitrogen,) i.e supplemented with 10% FCS (Thermo Scientific), L-Glutamine (Gibco), Non-Essential Amino Acids, Sodium Pyruvate, HEPES (Gibco) and Penicillin/Streptomycin (Gibco), at 37 ⁰ C in 5% C0 ₂ . For cytotoxicity assays and IL-12/IL-18 titration experiments, primary NK cells were harvested from the spleen, sorted using a mouse NK cell isolation kit (Miltenyi Biotec) and AutoMACS (Miltenyi Biotec), and subsequently cultured for 5 days in RPMI Medium supplemented with 10% FCS, L-Glutamine, Penicillin/Streptomycin, Non-Essential Amino Acids (Gibco), Sodium Pyruvate (Gibco), HEPES (Gibco), β- 2-mercaptoethanol (Calbiochem), and 1000 IU/ml recombinant human IL-2 (Chiron Corporation). All cells were incubated at 37°C in 5% C0 ₂ .

In vivo LPS challenges

LPS (from E. Coli 0127:B8, Sigma) suspended in PBS was injected intraperitoneally into mice at the described doses. For survival curves, mice were checked hourly for symptoms of sepsis. Serum from these mice was taken at various time points by retro-orbital or cardiac bleeding for cytokine analysis. Spleens were Jt"

20

also taken from mice at various time points to analyse receptor and ligand expression, and intracellular IFN-γ expression from NK cells.

In vivo tumor challenges

Mouse B16F10 or B16-OVA melanomas, RM-1 prostate carcinoma, 3LL lung carcinoma, MC38-OVA ^d,m colon adenocarcinoma or AT3-OVA ^dim mammary carcinoma, were injected into WT, DNAM-l ^7" , CD96 ^~ '\ or ϋΝΑΜ-ΐ ' θ^ό ' ^" mice subcutaneously or intravenously at the indicated doses and monitored for solid tumor growth or metastasis, respectively. Treatments were administered as indicated in the Figure legends. To monitor solid tumor growth, the area of the ensuing tumor was calculated by taking the length and width of palpable tumors by calipers and plotted against time. To monitor metastasis formation, 14 days after cells were injected, lungs were harvested, placed in Bouin's fixative, and metastases were counted using a dissection microscope.

MCA-induced fibrosarcoma

WT, DNAM-l ^" ' ^" , CD96 ^"A and DNAM- 1 ^~ ' ^~ CO96 ^~ ' ^~ mice were injected subcutaneously on the right flank with various doses of MCA (5-400 g, e.g. 100 μg MCA) and were monitored over time for fibrosarcoma formation. In addition, some mice were treated with control antibody, depleted of NK cells by treatment with anti- asialoGMl (Wako Chemicals; 100 μg injected i.p. at day -1, 0 and then weekly until week 8), neutralized for IFN-γ (H22, 250 μg injected i.p. at day -1, 0 and then weekly until week 8), for CD155, for DNAM-1 or CD96. Dendritic cells (BMDC): NK cell coculture assays

BMDC were generated as described previously. Briefly, we harvested bone marrow cells from the femur and tibia of mice and cultured them in DMEM supplemented with 10% FCS, L-Glutamine, Penicillin Streptomycin, Non-Essential Amino Acids, Sodium Pyruvate, β-2-mercaptoethanol and 250 ng/ml GM-CSF (eBioscience) for 6 days. WT or CD96 ^"A NK cells were harvested from the spleens and FACS sorted to purity by staining with NK1.1 (PK136) and TCRp (H57-597) and CD3 (17A2) antibodies. NK cells were harvested on the day of the assay. For assay set up, 5 x 10 ⁴ BMDM were plated in 96 well U bottom plates. NK cells were then added to the BMDM at varying titrations (2:1, 1:1, 0.5:1, and 0.25:1). BMDM only and NK only were always included in the assay as controls. Once all cells were plated, each well was filled with the appropriate amount of media to yield equivalent volumes between wells. 100 ng ml of LPS was then added to the wells for 2 h, followed by 5 mM purified ATP (Sigma) for 30 mins. This was performed at 37°C in 5% C0 ₂ . LPS only and ATP only controls were also included in the assay as controls. After 30 mins with ATP, supernatants were harvested and stored at -20°C until analysed. ⁵¹ Cr Cytotoxicity Assays

Standard ^5I Cr cytotoxicity assays were used to analyse the ability of WT and CD96 ^{" '} NK cells to kill targets. Briefly 20,000 targets labeled with 100 μθί of ⁵¹ Cr were added to V bottom plates and NK cells were then added to the targets at defined effector to target ratios. After 4 h at 37°C in 5% C0 ₂ , supernatants were harvested, and the level of ⁵¹ Cr was quantified by a gamma counter (Wallac Wizard). Percentage specific killing was determined using the formula (Sample Cr release- Spontaneous Cr release)/(Total Cr release-Spontaneous Cr release) x 100.

Cytokine Detection

All cytokine detection in serum or supernatants except IL-18 was achieved by utilising Cytometric Bead Array (CBA) technology (BD Biosciences). Acquisition was completed using a Canto II or LS II Flow Cytometric Analyser (BD Biosciences). Analysis was performed using the FCAP array software. IL-18 was detected by an ELISA according to manufacturer's instructions (MRL). For intracellular cytokine detection, isolated lymphocytes were obtained from the liver, stained for surface markers, fixed and permeabilised (BD Biosciences), and stained with an anti-IFN-γ antibody (XMG1.2).

Flow Cytometry Analysis and Sorting

Analysis of Immune Cell Homeostasis and CD96/CD 155 expression: Various organs (lymph node, lung, spleen, bone marrow, and liver) were processed into single lymphocyte suspensions that included red blood cell lysis. Between 1 x 10 ⁶ and 5 x 10 ⁶ cells were initially subject to incubation with 2.4G2 to block non-specific Fc antibody binding before specific antibodies were utilised. To analyse NK cell homeostasis and IFN-γ production, the following antibodies were used: anti mouse- NK1.1, -TCRp, -CD27 (LG.7F9), -CDl lb (Ml/70), and -IFN-γ. For T cells: anti mouse- TCRp, -CD8 (53-6.7), and -CD4 (RM4-5). For B cells: anti mouse -B220 (RA3-6B2), -CD19 (1D3). For NKT cells: mouse CDld tetramer loaded with a- galactosylceramide (kindly provided by Professor Dale Godfrey, University of Melbourne), anti mouse- TCRp or -CD3, -CD4, and -NK1.1. For macrophages: anti mouse- F4/80 (BM8) and -CDl lb. For neutrophils: anti mouse- Ly6G (1A8) and - CDl lb. For conventional DC: anti mouse- MHC II (M5/114.15.2) and -CDl lc (N418). For γδ T cells: anti mouse -γδ TCR (GL3) and -CD3. To analyse CD96 and CD 155 expression, the specific cell type of interest was gated upon using the above antibody cocktails along with anti mouse- CD96 (3.3.3) or anti mouse- CD 155 (4.24.3). Acquisition was performed using an LSR II, or Canto II flow cytometric analyser (BD Biosciences). Analysis was achieved using Flowjo (Treestar).

Cell Sorting

Naive NK cells and macrophages from the spleen were prepared and stained for as described above. These cells were then sorted to purity using an Aria II FACS sorter (BD Biosciences).

Statistical Analysis

Statistical analysis was achieved using Graphpad Prism Software. Data was considered to be statistically significant where the p value was equal to or less than 0.05. Statistical tests used were the unpaired Student's t test, Mann Whitney t test, and the Mantel-Cox test for survival. The appropriate test used is defined in the Figure legends.

Results

CD96 competes with DNAM-1 for CD 155 binding (Figure 1) and CD96 engagement by CD 155 down-regulates NK cell production of IFNy (Figure 2). CD96 limits NK cell-dependent tumor immunosurveillance in MCA-treated mice and promotes experimental B 16F10 lung metastasis (Figure 3). The data in Figure 4 show that anti-CD96 mAb has single agent activity (i.e without anti-PDl treatment) while also enhancing the anti-tumor responses of anti- PD1. Anti-CD96 mAb treatment also enhances anti-tumor responses generated by Doxorubicin (DOX) chemotherapy (Figures 5 & 7) which is consistent with Figure 6 where enhanced anti-tumor responses to Doxorubicin (DOX) chemotherapy were observed in host with CD96 deficiency. Referring to Figures 8 & 9, given early or late, anti-CD96 mAb enhances anti-tumor responses generated by anti-PD-1 and anti-CTLA-4 mAbs and shows a particularly strong synergy with anti-PD-1.

The effect of CD96 in promotion of tumour metastasis was also investigated. In Figure 10, regulation of B16F10 lung metastasis was investigated in C57BL/6 wild type (WT), DNAM-l ^" ' ^* , CD96 ^7' , and DNAM-1 ^/' CD96 ^{" "} mice In Figure 11, host CD96 promoted RM-1 lung metastasis and in Figure 12, host CD96 promoted 3LL lung metastasis Figure 13 shows that anti-CD96 mAb suppresses B16F10 lung metastasis, alone and in combination with a T cell checkpoint blockade. In Figure 14, anti-CD96 mAb suppresses RM-1 lung metastasis, alone and in combination with T cell checkpoint blockade. In Figure 15, anti-CD96 mAb enhances anti-tumor responses generated by anti-PD-1 and anti-CTLA-4 mAbs against MC38 colon tumors and shows a particularly strong synergy with anti-PD-l .

Example 2

Screening assays for identifying anti-CD96 antibodies

Introduction

The following assays may be used to identify antibodies useful in the invention. The first assay would be used to identify human antibodies capable of blocking or inhibiting binding between human CD96 and human CD 155. The second assay may be used to test whether or not the identified antibodies cause antibody-dependent cell-mediated cytotoxicity (ADCC). The third assay can then be applied to lead candidates and involves determining whether or not a human CD96 antibody can modulate human lymphocyte effector function.

Materials and Methods

Assay 1: CD96 binding to CD155

The ability of candidate anti-CD96 antibodies to prevent the binding of CD 155 to the cell surface of CD96 expressing cells (such as NK cells) will be tested rv.

24

as follows. Recombinant Human CD 155 fused to the C terminal Fc region of human IgGl (such as CD155-Fc available from Sino Biological) will be labeled with a fluorophore such as Alexa Fluor 647 (AF647) using Zenon Human IgG Labeling kit (Molecular Probe) accordingly to the manufacturer's instructions. NK cells or other CD96-expressing cells freshly isolated from the peripheral blood of healthy donors will be incubated with AF647 labeled CD155-Fc in the presence of anti-CD96 or control Ig at different concentrations ( The cells will be harvested and the cell surface binding of AF647-CD155-Fc will be tested by flow cytometry). Antibodies that prevent binding of CD 155 cells to CD96-expressing cells will be identified by their ability to block binding of CD 155-Fc to CD96-expressing cells.

Assay 2: ADCC assay

The survival of immune cells (such as NK cells and or T cells) in the presence of anti-CD96 antibodies will be analyzed as follows. The peripheral blood immune cells from healthy donors will be isolated by Ficoll gradient separation. Immune cells will be plated in 96 well plates in the presence of human IL-2 at an appropriate dosage and increasing concentrations of anti-CD96 mAbs. The survival as well as the percentages of CD96 expressing cells (such as NK cells and/or T cells) will be analyzed over time by flow cytometry. A non-limiting example of a suitable commercially available kit for this assay is the Annexin V Apoptosis Detection Kit.

Assay 3: Assay for modulation of human leukocyte effector function by human CD96 antibodies

Fresh blood samples will be collected from healthy donors. Peripheral blood mononuclear cells (PBMC) will be prepared on a Ficoll-Paque density gradient by centrifugation. Highly pure CD3-CD56+ NK cells will be obtained from PBMC by magnetically activated cell sorting. To analyze the ability of CD96 to impact human NK cell production of IFN-γ, 96 well U bottom plates will be coated overnight at 4°C with recombinant Human CD 155-Fc chimera (Sinb Biological Inc.; 0.5 μg/ well) or with non-relevant human IgGl antibodies. Freshly purified human NK cells will then be plated in complete RMPI media supplemented with Human IL-12 and IL-18 for 24 h and the intracellular content and the level of IFN-γ in the supernatant will be analysed in the different cultures. Alternatively, human NK cells will be stimulated for 24 h in wells coated with anti-N G2D, anti-NKp46, anti-NKp30 or anti-CD16 antibodies to analyze the ability of CD96 signalling to interact with other NK cells receptors. The anti-human CD96 antibodies to be tested or control antibodies will be added to the cultures prior to the cytokines or antibodies above to ^r confirm the ability of these test anti-human CD96 antibodies to enhance the IFNy production of the human NK cells. Statistical increases in IFNy production above the control would be considered significant.

Throughout the specification the aim has been to describe the preferred embodiments of the invention without limiting the invention to any one embodiment or specific collection of features. It will therefore be appreciated by those of skill in the art that, in light of the instant disclosure, various modifications and changes can be made in the particular embodiments exemplified without departing from the scope of the present invention.

All computer programs, algorithms, patent and scientific literature referred to herein is incorporated herein by reference.

f

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1. Seth, S., et al. The murine pan T cell marker CD96 is an adhesion receptor for CD155 and nectin-1. Biochemical and biophysical research communications 364, 959-965 (2007).

2. Fuchs, A., Cella, M., Giurisato, E., Shaw, A.S. & Colonna, M. Cutting edge:

CD96 (tactile) promotes NK cell-target cell adhesion by interacting with the poliovirus receptor (CD 155). J Immunol 172, 3994-3998 (2004).