Title:
IMPROVED AUTOINDUCTION EXPRESSION SYSTEM
Document Type and Number:
WIPO Patent Application WO/2011/062414
Kind Code:
A2
Abstract:
Provided is an autoinduction expression system in which variations are applied to Acs (acetyl-CoA synthase) gene promoters (mutagenesis) and an Acs promoter library is built up, and which shows more diverse expression aspects (expression volume and expression profile) than natural-occurring Acs promoters. The autoinduction expression of the present invention takes place with adjustment of the intracellular network for sensing the energy and nutritional states. In contrast to induction methods of the prior art which necessitate the monitoring of respective culture growths and the addition of inducers (IPTG or lactose) at appropriate times, the autoinduction expression system of the present invention can be implemented very simply, and allows target proteins to be obtained in large volume or with diverse profiles when cells which have been genetically transformed using an autoinduction expression vector of the present invention are inoculated into a culture medium having a carbon source (e.g. glucose) and culturing is carried out up until the stationary phase is reached.
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Inventors:
PAN JAE GU (KR)
KIM JUNG AE (KR)
KIM EUI JOONG (KR)
KIM JUNG AE (KR)
KIM EUI JOONG (KR)
Application Number:
PCT/KR2010/008137
Publication Date:
May 26, 2011
Filing Date:
November 17, 2010
Export Citation:
Assignee:
GENOFOCUS CO LTD (KR)
PAN JAE GU (KR)
KIM JUNG AE (KR)
KIM EUI JOONG (KR)
PAN JAE GU (KR)
KIM JUNG AE (KR)
KIM EUI JOONG (KR)
International Classes:
C12N15/63; C12N15/64; C12N15/70
Foreign References:
| US20040180423A1 | 2004-09-16 |
Other References:
BAN, JAE GU ET AL.: 'Autoinduction Expression System in Synthetic Biology Era' KSBB CONFERENCE April 2009, page 60
KIM, UI JUNG ET AL. 2ND KRIBB POSTER FESTIVAL ABSTRACTS 12 November 2008, page 58, A-61
CADWELL, R.C. ET AL.: 'Randomization of genes by PCR mutagenesis' GENOME RESEARCH vol. 2, no. 1, August 1992, pages 28 - 33
SIVASHANMUGAM, A. ET AL.: 'Practical protocols for production of very high yields of recombinant proteins using Escherichia coli' PROTEIN SCIENCE vol. 18, no. 5, May 2009, pages 936 - 948
KIM, UI JUNG ET AL. 2ND KRIBB POSTER FESTIVAL ABSTRACTS 12 November 2008, page 58, A-61
CADWELL, R.C. ET AL.: 'Randomization of genes by PCR mutagenesis' GENOME RESEARCH vol. 2, no. 1, August 1992, pages 28 - 33
SIVASHANMUGAM, A. ET AL.: 'Practical protocols for production of very high yields of recombinant proteins using Escherichia coli' PROTEIN SCIENCE vol. 18, no. 5, May 2009, pages 936 - 948
Attorney, Agent or Firm:
YANG, Boo-Hyun (KR)
μλΆν (KR)
μλΆν (KR)
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