Title:
IMPROVED GRANZYME B VARIANT
Document Type and Number:
WIPO Patent Application WO/2021/241719
Kind Code:
A1
Abstract:
The present invention pertains to: a granzyme B variant having enhanced protease activity and/or enhanced tolerance against an inhibitory factor; a polynucleotide encoding the granzyme B variant; a cell expressing the granzyme B variant; a pharmaceutical composition comprising the cell expressing the granzyme B variant; and a pharmaceutical composition comprising the granzyme B variant. In some embodiments, the pharmaceutical composition can be used in combination with a cell expressing a chimeric receptor and/or an antigen-binding molecule.
More Like This:
Inventors:
KOMORI YASUNORI (JP)
IGAWA TOMOYUKI (SG)
NARITA ATSUSHI (JP)
ISHII SHINYA (JP)
IGAWA TOMOYUKI (SG)
NARITA ATSUSHI (JP)
ISHII SHINYA (JP)
Application Number:
PCT/JP2021/020334
Publication Date:
December 02, 2021
Filing Date:
May 28, 2021
Export Citation:
Assignee:
CHUGAI PHARMACEUTICAL CO LTD (JP)
International Classes:
A61K39/395; A61K35/12; A61P35/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/64; C12N15/12; C12N15/57; C12N15/63; A61K38/48; A61P29/00; A61P43/00
Domestic Patent References:
| WO2013041659A2 | 2013-03-28 | |||
| WO2005100556A2 | 2005-10-27 | |||
| WO2020169620A1 | 2020-08-27 | |||
| WO2006026451A2 | 2006-03-09 | |||
| WO2017143026A1 | 2017-08-24 |
Foreign References:
| JP2015532102A | 2015-11-09 | |||
| JP2019526285A | 2019-09-19 |
Other References:
LOSASSO, V. ET AL.: "Design of human granzyme B variants resistant to serpin B9", PROTEINS, vol. 80, 2012, pages 2514 - 2522, XP055094445, DOI: 10.1002/prot.24133
SCHIFFER S, HANSEN H P, HEHMANN-TITT G, HUHN M, FISCHER R, BARTH S, THEPEN T: "Efficacy of an adapted granzyme B-based anti-CD30 cytolytic fusion protein against PI-9-positive classical Hodgkin lymphoma cells in a murine model", BLOOD CANCER JOURNAL, vol. 3, no. 3, 1 March 2013 (2013-03-01), pages e106 - e106, XP055880528, DOI: 10.1038/bcj.2013.4
HEHMANN-TITT, G. ET AL.: "Improving the therapeutic potential of human granzyme B for targeted cancer therap y", ANTIBODIES, vol. 2, 2013, pages 19 - 49, XP002747507, DOI: 10.3390/antib2010019
HLONGWANE PRECIOUS, MUNGRA NEELAKSHI, MADHESWARAN SURESH, AKINRINMADE OLUSIJI, CHETTY SHIVAN, BARTH STEFAN: "Human Granzyme B Based Targeted Cytolytic Fusion Proteins", BIOMEDICINES, MDPI AG, vol. 6, no. 2, 20 June 2018 (2018-06-20), pages 72, XP055789608, DOI: 10.3390/biomedicines6020072
GRAHAM, L. D. ET AL.: "Random mutagenesis of the substrate-binding site of a serine protease can generate enzymes with increased activities and altered primary specificities", BIOCHEMISTRY, vol. 32, 1993, pages 6250 - 6258, XP055043971, DOI: 10.1021/bi00075a019
OBEROI PRANAV, WELS WINFRIED S: "Arming NK cells with enhanced antitumor activity : CARs and beyond", ONCOIMMUNOLOGY, vol. 2, no. 8, 1 August 2013 (2013-08-01), pages e25220, XP055880707, DOI: 10.4161/onci.25220
OBEROI PRANAV, JABULOWSKY ROBERT A., BÄHR-MAHMUD HAYAT, WELS WINFRIED S.: "EGFR-Targeted Granzyme B Expressed in NK Cells Enhances Natural Cytotoxicity and Mediates Specific Killing of Tumor Cells", PLOS ONE, PUBLIC LIBRARY OF SCIENCE, vol. 8, no. 4, 3 April 2013 (2013-04-03), pages e61267, XP055880719, DOI: 10.1371/journal.pone.0061267
QINGHUA HE, XIANHAN JIANG, XINKE ZHOU, JINSHENG WENG: "Targeting cancers through TCR-peptide/MHC interactions", JOURNAL OF HEMATOLOGY & ONCOLOGY, vol. 12, no. 1, 1 December 2019 (2019-12-01), XP055679806, DOI: 10.1186/s13045-019-0812-8
ANDONIOU CHRISTOPHER E., SUTTON VIVIEN R., WIKSTROM MATTHEW E., FLEMING PETER, THIA KEVIN Y. T., MATTHEWS ANTONY Y., KAISERMAN DIO: "A Natural Genetic Variant of Granzyme B Confers Lethality to a Common Viral Infection", PLOS PATHOGENS, PUBLIC LIBRARY OF SCIENCE, US, vol. 10, no. 12, 11 December 2014 (2014-12-11), US , pages e1004526, XP055880723, ISSN: 1553-7366, DOI: 10.1371/journal.ppat.1004526
CULLEN SEAN P., ADRAIN COLIN, LÜTHI ALEXANDER U., DURIEZ PATRICK J., MARTIN SEAMUS J.: "Human and murine granzyme B exhibit divergent substrate preferences", THE JOURNAL OF CELL BIOLOGY, THE ROCKEFELLER UNIVERSITY PRESS, US, vol. 176, no. 4, 12 February 2007 (2007-02-12), US , pages 435 - 444, XP055880726, ISSN: 0021-9525, DOI: 10.1083/jcb.200612025
BOTS MICHAEL, VAN BOSTELEN LIESBETH, RADEMAKER MIRJAM TGA, OFFRINGA RIENK, MEDEMA JAN PAUL: "Serpins prevent granzyme‐induced death in a species‐specific manner", IMMUNOLOGY AND CELL BIOLOGY, CARLTON, AU, vol. 84, no. 1, 1 February 2006 (2006-02-01), AU , pages 79 - 86, XP055881089, ISSN: 0818-9641, DOI: 10.1111/j.1440-1711.2005.01417.x
CASCIOLA-ROSEN LIVIA, GARCIA-CALVO MARGARITA, BULL HERBERT G., BECKER JOSEPH W., HINES TONIE, THORNBERRY NANCY A., ROSEN ANTONY: "Mouse and Human Granzyme B Have Distinct Tetrapeptide Specificities and Abilities to Recruit the Bid Pathway", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, US, vol. 282, no. 7, 1 February 2007 (2007-02-01), US , pages 4545 - 4552, XP055881090, ISSN: 0021-9258, DOI: 10.1074/jbc.M606564200
VAN DAMME PETRA, MAURER-STROH SEBASTIAN, PLASMAN KIM, VAN DURME JOOST, COLAERT NIKLAAS, TIMMERMAN EVY, DE BOCK PIETER-JAN, GOETHAL: "Analysis of Protein Processing by N-terminal Proteomics Reveals Novel Species-specific Substrate Determinants of Granzyme B Orthologs", MOLECULAR & CELLULAR PROTEOMICS, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, US, vol. 8, no. 2, 1 February 2009 (2009-02-01), US , pages 258 - 272, XP055881091, ISSN: 1535-9476, DOI: 10.1074/mcp.M800060-MCP200
OLADAPO O. YEKU, TERENCE J. PURDON, MYTHILI KONERU, DAVID SPRIGGS, RENIER J. BRENTJENS: "Armored CAR T cells enhance antitumor efficacy and overcome the tumor microenvironment", SCIENTIFIC REPORTS, vol. 7, no. 1, 1 December 2017 (2017-12-01), XP055579095, DOI: 10.1038/s41598-017-10940-8
CHEN MUHUA, SUN RUIXIN, SHI BIZHI, WANG YI, DI SHENGMENG, LUO HONG, SUN YANSHA, LI ZONGHAI, ZHOU MIN, JIANG HUA: "Antitumor efficacy of chimeric antigen receptor T cells against EGFRvIII-expressing glioblastoma in C57BL/6 mice", BIOMEDICINE & PHARMACOTHERAPY, ELSEVIER, FR, vol. 113, 1 May 2019 (2019-05-01), FR , pages 108734, XP055881094, ISSN: 0753-3322, DOI: 10.1016/j.biopha.2019.108734
DEROUAZI, M. ET AL.: "Novel cell -penetrating peptide-based vaccine induces robust CD 4+ and CD 8+ T cell -mediated antitumor immunity", CANCER RES., vol. 75, no. 15, 2015, pages 3020 - 3031, XP055227770, DOI: 10.1158/0008-5472.CAN-14-3017
SINGER J., JENSEN‐JAROLIM E.: "IgE‐based immunotherapy of cancer: challenges and chances", ALLERGY, WILEY-BLACKWELL PUBLISHING LTD., UNITED KINGDOM, vol. 69, no. 2, 1 February 2014 (2014-02-01), United Kingdom , pages 137 - 149, XP055880517, ISSN: 0105-4538, DOI: 10.1111/all.12276
NAKAJIMA H, PARK H L, HENKART P A: "Synergistic roles of granzymes A and B in mediating target cell death by rat basophilic leukemia mast cell tumors also expressing cytolysin/perforin.", JOURNAL OF EXPERIMENTAL MEDICINE, ROCKEFELLER UNIVERSITY PRESS, US, vol. 181, no. 3, 1 March 1995 (1995-03-01), US , pages 1037 - 1046, XP055880515, ISSN: 0022-1007, DOI: 10.1084/jem.181.3.1037
SMITH, A. J.: "New horizons in therapeutic antibody discovery: opportunities and challenges versus small-molecule therapeutics", J. BIOMOL. SCREEN., vol. 20, no. 4, 2015, pages 437 - 453, XP009184906, DOI: 10.1177/1087057114562544
RUGGLES SANDRA WAUGH ET AL: "Characterization of structural determinants of granzyme B reveals potent mediators of extended substrate specificity.", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, US, vol. 279, no. 29, 16 July 2004 (2004-07-16), US , pages 30751 - 30759, XP002304824, ISSN: 0021-9258, DOI: 10.1074/jbc.M400949200
PIUKO, K. ET AL.: "Identification and characterization of equine granzyme B", VET. IMMUNOL. IMMUNOPATHOL., vol. 118, 2007, pages 239 - 251, XP022153308, DOI: 10.1016/j.vetimm.2007.05.002
FU ZHIRONG, THORPE MICHAEL, AKULA SRINIVAS, HELLMAN LARS: "Asp-ase Activity of the Opossum Granzyme B Supports the Role of Granzyme B as Part of Anti-Viral Immunity Already during Early Mammalian Evolution", PLOS ONE, PUBLIC LIBRARY OF SCIENCE, vol. 11, no. 5, 6 May 2016 (2016-05-06), pages e0154886, XP055880513, DOI: 10.1371/journal.pone.0154886
ADAM J ERICSEN;GABRIEL J STARRETT;JUSTIN M GREENE;MICHAEL LAUCK;MUTHUSWAMY RAVEENDRAN;DAVID RIO DEIROS;MARIEL S MOHNS;NICOLAS VINC: "Whole genome sequencing of SIV-infected macaques identifies candidate loci that may contribute to host control of virus replication", GENOME BIOLOGY, BIOMED CENTRAL LTD., vol. 15, no. 11, 7 November 2014 (2014-11-07), pages 478, XP021202977, ISSN: 1465-6906, DOI: 10.1186/s13059-014-0478-z
DATABASE Nucleotide [online] 13 November 2014 (2014-11-13), "Macaca fascicularis granzyme B (GzmB) gene, GzmB- 1:01:01 allele, complete cds", XP055881103, retrieved from ncbi Database accession no. KM281203.1
DATABASE Protein [online] 1 June 2015 (2015-06-01), "PREDICTED: granzyme B [Propithecus coquereli]", XP055881108, retrieved from ncbi Database accession no. XP_012502759.1
DATABASE Protein [online] 19 March 2015 (2015-03-19), "Granzyme B [Tupaia chinensis]", XP055881111, retrieved from ncbi Database accession no. ELW69303.1
DATABASE Protein [online] 21 March 2019 (2019-03-21), "Granzyme B isoform X1 [Physeter catodon]", XP055881137, retrieved from ncbi Database accession no. XP_007119743.1
DATABASE Protein [online] 16 September 2019 (2019-09-16), "Granzyme B-like isoform XI [Delphinapterus leucas]", XP055881138, retrieved from ncbi Database accession no. XP_022409388.1
DATABASE Protein [online] 18 April 2018 (2018-04-18), "Granzyme B-like isoform XI [Neophocaena asiaeorientalis asiaeorientalis]", XP055881098, retrieved from ncbi Database accession no. XP_024624959.1
DATABASE Protein [online] 1 April 2014 (2014-04-01), "PREDICTED: granzyme B [Lipotes vexillifer]", XP055881142, retrieved from ncbi Database accession no. XP_007471896.1
DATABASE Protein [online] 27 April 2020 (2020-04-27), "Granzyme B [Tursiops truncatus]", XP055881145, retrieved from ncbi Database accession no. XP_033708488.1
DATABASE Protein [online] 31 December 2014 (2014-12-31), "PREDICTED: granzyme B-like [Bison bison bison]", XP055881150, retrieved from ncbi Database accession no. XP_010841689.1
DATABASE Protein [online] 8 September 2016 (2016-09-08), "PREDICTED: granzyme B [Capra hircus]", XP055881152, retrieved from ncbi Database accession no. XP_017900778.1
DATABASE Protein [online] 6 February 2019 (2019-02-06), "Granzyme B-like [Ovis aries]", XP055881154, retrieved from ncbi Database accession no. XP_027813308.1
DATABASE Protein [online] 19 March 2015 (2015-03-19), "Granzyme B, partial [Bos mutus]", XP055881157, retrieved from ncbi Database accession no. ELR49680.1
DATABASE Protein [online] 4 October 2019 (2019-10-04), "Granzyme B [Camelus dromedarius]", XP055881160, retrieved from ncbi Database accession no. KAB1278066.1
DATABASE UniProtKB [online] 22 April 2020 (2020-04-22), "Granzyme B", XP055881171, Database accession no. A0A2F0B3Z3
DATABASE Protein [online] 26 June 2015 (2015-06-26), "PREDICTED: granzyme B-like [Dipodomys ordii]", XP055882146, retrieved from ncbi Database accession no. XP_012880796.1
DATABASE Protein [online] 26 June 2015 (2015-06-26), "PREDICTED: granzyme B-like [Dipodomys ordii]", XP055882148, retrieved from ncbi Database accession no. XP_012880811.1
DATABASE UniProtKB [online] 26 February 2020 (2020-02-26), "Granzyme B", XP055882151, retrieved from UniProt Database accession no. A0A2K5YY00
DATABASE Protein [online] 30 March 2015 (2015-03-30), "PREDICTED: granzyme B [Cercocebus atys]", XP055882156, retrieved from ncbi Database accession no. XP_011946523.1
DATABASE Protein [online] 28 June 2017 (2017-06-28), "Granzyme B [Aotus nancymaae]", XP055882159, retrieved from ncbi Database accession no. XP_012323554.1
DATABASE Protein [online] 24 November 2014 (2014-11-24), "Granzyme B [Saimiri boliviensis boliviensis]", XP055882163, retrieved from ncbi Database accession no. XP_003924294.2
DATABASE Protein [online] 30 September 2019 (2019-09-30), "Granzyme B isoform XI [Gorilla gorilla gorilla]", XP055882165, retrieved from ncbi Database accession no. XP_004055081.1
DATABASE Protein [online] 25 January 2018 (2018-01-25), "Granzyme B [Trichechus manatus latirostris", XP055882166, retrieved from ncbi Database accession no. XP_004376613.1
YANG JIE, ALAN PEMBERTON, W. IVAN MORRISON, TIM CONNELLEY: "Granzyme B is an essential mediator in CD 8 T cell killing of theileria parva-infected cell s", INFECT. IMMUN., vol. 87, no. 1, 1 January 2019 (2019-01-01), pages e00386 - 18, XP055880505
LUIS MARTINEZ-LOSTAO, ALBERTO ANEL,JULIAN PARDO: " How Do Cytotoxic Lymphocytes Kill Cancer Cells?", CLIN CANCER RES., no. 22, 15 November 2015 (2015-11-15), pages 5047 - 56
JENNE DE: "Delivery and therapeutic potential of human granzyme B Kurschus FC1", IMMUNOL REV, vol. 235, no. 1, May 2010 (2010-05-01), pages 159 - 71, XP055179509, DOI: 10.1111/j.0105-2896.2010.00894.x
J. P. MEDEMA, J. DE JONG, L. T. C. PELTENBURG, E. M. E. VERDEGAAL, A. GORTER, S. A. BRES, K. L. M. C. FRANKEN, M. HAHNE, J. P. ALB: "Blockade of the granzyme B/perforin pathway through overexpression of the serine protease inhibitor PI-9/SPI-6 constitutes a mechanism for immune escape by tumors ", PROC NATL ACAD SCI USA., vol. 98, no. 20, 25 September 2001 (2001-09-25), pages 11515 - 11520
CATHERINA H. BIRD, JIURU SUN, KHENG UNG, DIANA KARAMBALIS, JAMES C. WHISSTOCK, JOSEPH A.,TRAPANI, PHILLIP I. BIRD: "ationic Sites on Granzyme B Contribute to Cytotoxicity by Promoting Its Uptake into Target Cells ", MOL CELL BIOL, vol. 25, no. 17, September 2005 (2005-09-01), pages 7854 - 7867
LOSASSO V, SCHIFER S, BARTH S, CARLONI P: "Design of human granzyme B variants resistant to serpin B9 ", PROTEINS, vol. 80, no. 11, November 2012 (2012-11-01), pages 2514 - 22, XP055094445, DOI: 10.1002/prot.24133
KURSCHUS FC, FELLOWS E, STEGMANN E, JENNE DE: "Granzyme B delivery via perforin is restricted by size, but not by heparan sulfate-dependent endocytosis", PROC NATL ACAD SCI USA., vol. 105, no. 37, 16 September 2008 (2008-09-16), pages 13799 - 13804, XP009106108, DOI: 10.1073/pnas.0801724105
SINGLETON ET AL.: "Dictionary of Microbiology and Molecular Biology", 1994, J. WILEY & SONS
MARCH: "Mechanisms and Structure", vol. Advanced Organic Chemistry Reactions, 1992, JOHN WILEY & SONS
KINDT ET AL.: "Kuby Immunology", 2007, W.H. FREEMAN AND CO, pages: 91
PORTOLANO ET AL., J. IMMUNOL., vol. 150, 1993, pages 880 - 887
CLARKSON ET AL., NATURE, vol. 352, 1991, pages 624 - 628
J VIS EXP, no. 100, 10 June 2015 (2015-06-10), pages e52911
PROTEIN SCIENCE, vol. 4, 1995, pages 2411 - 2423
JOHNSON ET AL., BLOOD, vol. 114, 2009, pages 535 - 546
SCHIFFER S, HANSEN H P, HEHMANN-TITT G, HUHN M, FISCHER R, BARTH S, THEPEN T: "Efficacy of an adapted granzyme B-based anti-CD30 cytolytic fusion protein against PI-9-positive classical Hodgkin lymphoma cells in a murine model", BLOOD CANCER JOURNAL, vol. 3, no. 3, 1 March 2013 (2013-03-01), pages e106 - e106, XP055880528, DOI: 10.1038/bcj.2013.4
HEHMANN-TITT, G. ET AL.: "Improving the therapeutic potential of human granzyme B for targeted cancer therap y", ANTIBODIES, vol. 2, 2013, pages 19 - 49, XP002747507, DOI: 10.3390/antib2010019
HLONGWANE PRECIOUS, MUNGRA NEELAKSHI, MADHESWARAN SURESH, AKINRINMADE OLUSIJI, CHETTY SHIVAN, BARTH STEFAN: "Human Granzyme B Based Targeted Cytolytic Fusion Proteins", BIOMEDICINES, MDPI AG, vol. 6, no. 2, 20 June 2018 (2018-06-20), pages 72, XP055789608, DOI: 10.3390/biomedicines6020072
GRAHAM, L. D. ET AL.: "Random mutagenesis of the substrate-binding site of a serine protease can generate enzymes with increased activities and altered primary specificities", BIOCHEMISTRY, vol. 32, 1993, pages 6250 - 6258, XP055043971, DOI: 10.1021/bi00075a019
OBEROI PRANAV, WELS WINFRIED S: "Arming NK cells with enhanced antitumor activity : CARs and beyond", ONCOIMMUNOLOGY, vol. 2, no. 8, 1 August 2013 (2013-08-01), pages e25220, XP055880707, DOI: 10.4161/onci.25220
OBEROI PRANAV, JABULOWSKY ROBERT A., BÄHR-MAHMUD HAYAT, WELS WINFRIED S.: "EGFR-Targeted Granzyme B Expressed in NK Cells Enhances Natural Cytotoxicity and Mediates Specific Killing of Tumor Cells", PLOS ONE, PUBLIC LIBRARY OF SCIENCE, vol. 8, no. 4, 3 April 2013 (2013-04-03), pages e61267, XP055880719, DOI: 10.1371/journal.pone.0061267
QINGHUA HE, XIANHAN JIANG, XINKE ZHOU, JINSHENG WENG: "Targeting cancers through TCR-peptide/MHC interactions", JOURNAL OF HEMATOLOGY & ONCOLOGY, vol. 12, no. 1, 1 December 2019 (2019-12-01), XP055679806, DOI: 10.1186/s13045-019-0812-8
ANDONIOU CHRISTOPHER E., SUTTON VIVIEN R., WIKSTROM MATTHEW E., FLEMING PETER, THIA KEVIN Y. T., MATTHEWS ANTONY Y., KAISERMAN DIO: "A Natural Genetic Variant of Granzyme B Confers Lethality to a Common Viral Infection", PLOS PATHOGENS, PUBLIC LIBRARY OF SCIENCE, US, vol. 10, no. 12, 11 December 2014 (2014-12-11), US , pages e1004526, XP055880723, ISSN: 1553-7366, DOI: 10.1371/journal.ppat.1004526
CULLEN SEAN P., ADRAIN COLIN, LÜTHI ALEXANDER U., DURIEZ PATRICK J., MARTIN SEAMUS J.: "Human and murine granzyme B exhibit divergent substrate preferences", THE JOURNAL OF CELL BIOLOGY, THE ROCKEFELLER UNIVERSITY PRESS, US, vol. 176, no. 4, 12 February 2007 (2007-02-12), US , pages 435 - 444, XP055880726, ISSN: 0021-9525, DOI: 10.1083/jcb.200612025
BOTS MICHAEL, VAN BOSTELEN LIESBETH, RADEMAKER MIRJAM TGA, OFFRINGA RIENK, MEDEMA JAN PAUL: "Serpins prevent granzyme‐induced death in a species‐specific manner", IMMUNOLOGY AND CELL BIOLOGY, CARLTON, AU, vol. 84, no. 1, 1 February 2006 (2006-02-01), AU , pages 79 - 86, XP055881089, ISSN: 0818-9641, DOI: 10.1111/j.1440-1711.2005.01417.x
CASCIOLA-ROSEN LIVIA, GARCIA-CALVO MARGARITA, BULL HERBERT G., BECKER JOSEPH W., HINES TONIE, THORNBERRY NANCY A., ROSEN ANTONY: "Mouse and Human Granzyme B Have Distinct Tetrapeptide Specificities and Abilities to Recruit the Bid Pathway", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, US, vol. 282, no. 7, 1 February 2007 (2007-02-01), US , pages 4545 - 4552, XP055881090, ISSN: 0021-9258, DOI: 10.1074/jbc.M606564200
VAN DAMME PETRA, MAURER-STROH SEBASTIAN, PLASMAN KIM, VAN DURME JOOST, COLAERT NIKLAAS, TIMMERMAN EVY, DE BOCK PIETER-JAN, GOETHAL: "Analysis of Protein Processing by N-terminal Proteomics Reveals Novel Species-specific Substrate Determinants of Granzyme B Orthologs", MOLECULAR & CELLULAR PROTEOMICS, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, US, vol. 8, no. 2, 1 February 2009 (2009-02-01), US , pages 258 - 272, XP055881091, ISSN: 1535-9476, DOI: 10.1074/mcp.M800060-MCP200
OLADAPO O. YEKU, TERENCE J. PURDON, MYTHILI KONERU, DAVID SPRIGGS, RENIER J. BRENTJENS: "Armored CAR T cells enhance antitumor efficacy and overcome the tumor microenvironment", SCIENTIFIC REPORTS, vol. 7, no. 1, 1 December 2017 (2017-12-01), XP055579095, DOI: 10.1038/s41598-017-10940-8
CHEN MUHUA, SUN RUIXIN, SHI BIZHI, WANG YI, DI SHENGMENG, LUO HONG, SUN YANSHA, LI ZONGHAI, ZHOU MIN, JIANG HUA: "Antitumor efficacy of chimeric antigen receptor T cells against EGFRvIII-expressing glioblastoma in C57BL/6 mice", BIOMEDICINE & PHARMACOTHERAPY, ELSEVIER, FR, vol. 113, 1 May 2019 (2019-05-01), FR , pages 108734, XP055881094, ISSN: 0753-3322, DOI: 10.1016/j.biopha.2019.108734
DEROUAZI, M. ET AL.: "Novel cell -penetrating peptide-based vaccine induces robust CD 4+ and CD 8+ T cell -mediated antitumor immunity", CANCER RES., vol. 75, no. 15, 2015, pages 3020 - 3031, XP055227770, DOI: 10.1158/0008-5472.CAN-14-3017
SINGER J., JENSEN‐JAROLIM E.: "IgE‐based immunotherapy of cancer: challenges and chances", ALLERGY, WILEY-BLACKWELL PUBLISHING LTD., UNITED KINGDOM, vol. 69, no. 2, 1 February 2014 (2014-02-01), United Kingdom , pages 137 - 149, XP055880517, ISSN: 0105-4538, DOI: 10.1111/all.12276
NAKAJIMA H, PARK H L, HENKART P A: "Synergistic roles of granzymes A and B in mediating target cell death by rat basophilic leukemia mast cell tumors also expressing cytolysin/perforin.", JOURNAL OF EXPERIMENTAL MEDICINE, ROCKEFELLER UNIVERSITY PRESS, US, vol. 181, no. 3, 1 March 1995 (1995-03-01), US , pages 1037 - 1046, XP055880515, ISSN: 0022-1007, DOI: 10.1084/jem.181.3.1037
SMITH, A. J.: "New horizons in therapeutic antibody discovery: opportunities and challenges versus small-molecule therapeutics", J. BIOMOL. SCREEN., vol. 20, no. 4, 2015, pages 437 - 453, XP009184906, DOI: 10.1177/1087057114562544
RUGGLES SANDRA WAUGH ET AL: "Characterization of structural determinants of granzyme B reveals potent mediators of extended substrate specificity.", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, US, vol. 279, no. 29, 16 July 2004 (2004-07-16), US , pages 30751 - 30759, XP002304824, ISSN: 0021-9258, DOI: 10.1074/jbc.M400949200
PIUKO, K. ET AL.: "Identification and characterization of equine granzyme B", VET. IMMUNOL. IMMUNOPATHOL., vol. 118, 2007, pages 239 - 251, XP022153308, DOI: 10.1016/j.vetimm.2007.05.002
FU ZHIRONG, THORPE MICHAEL, AKULA SRINIVAS, HELLMAN LARS: "Asp-ase Activity of the Opossum Granzyme B Supports the Role of Granzyme B as Part of Anti-Viral Immunity Already during Early Mammalian Evolution", PLOS ONE, PUBLIC LIBRARY OF SCIENCE, vol. 11, no. 5, 6 May 2016 (2016-05-06), pages e0154886, XP055880513, DOI: 10.1371/journal.pone.0154886
ADAM J ERICSEN;GABRIEL J STARRETT;JUSTIN M GREENE;MICHAEL LAUCK;MUTHUSWAMY RAVEENDRAN;DAVID RIO DEIROS;MARIEL S MOHNS;NICOLAS VINC: "Whole genome sequencing of SIV-infected macaques identifies candidate loci that may contribute to host control of virus replication", GENOME BIOLOGY, BIOMED CENTRAL LTD., vol. 15, no. 11, 7 November 2014 (2014-11-07), pages 478, XP021202977, ISSN: 1465-6906, DOI: 10.1186/s13059-014-0478-z
DATABASE Nucleotide [online] 13 November 2014 (2014-11-13), "Macaca fascicularis granzyme B (GzmB) gene, GzmB- 1:01:01 allele, complete cds", XP055881103, retrieved from ncbi Database accession no. KM281203.1
DATABASE Protein [online] 1 June 2015 (2015-06-01), "PREDICTED: granzyme B [Propithecus coquereli]", XP055881108, retrieved from ncbi Database accession no. XP_012502759.1
DATABASE Protein [online] 19 March 2015 (2015-03-19), "Granzyme B [Tupaia chinensis]", XP055881111, retrieved from ncbi Database accession no. ELW69303.1
DATABASE Protein [online] 21 March 2019 (2019-03-21), "Granzyme B isoform X1 [Physeter catodon]", XP055881137, retrieved from ncbi Database accession no. XP_007119743.1
DATABASE Protein [online] 16 September 2019 (2019-09-16), "Granzyme B-like isoform XI [Delphinapterus leucas]", XP055881138, retrieved from ncbi Database accession no. XP_022409388.1
DATABASE Protein [online] 18 April 2018 (2018-04-18), "Granzyme B-like isoform XI [Neophocaena asiaeorientalis asiaeorientalis]", XP055881098, retrieved from ncbi Database accession no. XP_024624959.1
DATABASE Protein [online] 1 April 2014 (2014-04-01), "PREDICTED: granzyme B [Lipotes vexillifer]", XP055881142, retrieved from ncbi Database accession no. XP_007471896.1
DATABASE Protein [online] 27 April 2020 (2020-04-27), "Granzyme B [Tursiops truncatus]", XP055881145, retrieved from ncbi Database accession no. XP_033708488.1
DATABASE Protein [online] 31 December 2014 (2014-12-31), "PREDICTED: granzyme B-like [Bison bison bison]", XP055881150, retrieved from ncbi Database accession no. XP_010841689.1
DATABASE Protein [online] 8 September 2016 (2016-09-08), "PREDICTED: granzyme B [Capra hircus]", XP055881152, retrieved from ncbi Database accession no. XP_017900778.1
DATABASE Protein [online] 6 February 2019 (2019-02-06), "Granzyme B-like [Ovis aries]", XP055881154, retrieved from ncbi Database accession no. XP_027813308.1
DATABASE Protein [online] 19 March 2015 (2015-03-19), "Granzyme B, partial [Bos mutus]", XP055881157, retrieved from ncbi Database accession no. ELR49680.1
DATABASE Protein [online] 4 October 2019 (2019-10-04), "Granzyme B [Camelus dromedarius]", XP055881160, retrieved from ncbi Database accession no. KAB1278066.1
DATABASE UniProtKB [online] 22 April 2020 (2020-04-22), "Granzyme B", XP055881171, Database accession no. A0A2F0B3Z3
DATABASE Protein [online] 26 June 2015 (2015-06-26), "PREDICTED: granzyme B-like [Dipodomys ordii]", XP055882146, retrieved from ncbi Database accession no. XP_012880796.1
DATABASE Protein [online] 26 June 2015 (2015-06-26), "PREDICTED: granzyme B-like [Dipodomys ordii]", XP055882148, retrieved from ncbi Database accession no. XP_012880811.1
DATABASE UniProtKB [online] 26 February 2020 (2020-02-26), "Granzyme B", XP055882151, retrieved from UniProt Database accession no. A0A2K5YY00
DATABASE Protein [online] 30 March 2015 (2015-03-30), "PREDICTED: granzyme B [Cercocebus atys]", XP055882156, retrieved from ncbi Database accession no. XP_011946523.1
DATABASE Protein [online] 28 June 2017 (2017-06-28), "Granzyme B [Aotus nancymaae]", XP055882159, retrieved from ncbi Database accession no. XP_012323554.1
DATABASE Protein [online] 24 November 2014 (2014-11-24), "Granzyme B [Saimiri boliviensis boliviensis]", XP055882163, retrieved from ncbi Database accession no. XP_003924294.2
DATABASE Protein [online] 30 September 2019 (2019-09-30), "Granzyme B isoform XI [Gorilla gorilla gorilla]", XP055882165, retrieved from ncbi Database accession no. XP_004055081.1
DATABASE Protein [online] 25 January 2018 (2018-01-25), "Granzyme B [Trichechus manatus latirostris", XP055882166, retrieved from ncbi Database accession no. XP_004376613.1
YANG JIE, ALAN PEMBERTON, W. IVAN MORRISON, TIM CONNELLEY: "Granzyme B is an essential mediator in CD 8 T cell killing of theileria parva-infected cell s", INFECT. IMMUN., vol. 87, no. 1, 1 January 2019 (2019-01-01), pages e00386 - 18, XP055880505
LUIS MARTINEZ-LOSTAO, ALBERTO ANEL,JULIAN PARDO: " How Do Cytotoxic Lymphocytes Kill Cancer Cells?", CLIN CANCER RES., no. 22, 15 November 2015 (2015-11-15), pages 5047 - 56
JENNE DE: "Delivery and therapeutic potential of human granzyme B Kurschus FC1", IMMUNOL REV, vol. 235, no. 1, May 2010 (2010-05-01), pages 159 - 71, XP055179509, DOI: 10.1111/j.0105-2896.2010.00894.x
J. P. MEDEMA, J. DE JONG, L. T. C. PELTENBURG, E. M. E. VERDEGAAL, A. GORTER, S. A. BRES, K. L. M. C. FRANKEN, M. HAHNE, J. P. ALB: "Blockade of the granzyme B/perforin pathway through overexpression of the serine protease inhibitor PI-9/SPI-6 constitutes a mechanism for immune escape by tumors ", PROC NATL ACAD SCI USA., vol. 98, no. 20, 25 September 2001 (2001-09-25), pages 11515 - 11520
CATHERINA H. BIRD, JIURU SUN, KHENG UNG, DIANA KARAMBALIS, JAMES C. WHISSTOCK, JOSEPH A.,TRAPANI, PHILLIP I. BIRD: "ationic Sites on Granzyme B Contribute to Cytotoxicity by Promoting Its Uptake into Target Cells ", MOL CELL BIOL, vol. 25, no. 17, September 2005 (2005-09-01), pages 7854 - 7867
LOSASSO V, SCHIFER S, BARTH S, CARLONI P: "Design of human granzyme B variants resistant to serpin B9 ", PROTEINS, vol. 80, no. 11, November 2012 (2012-11-01), pages 2514 - 22, XP055094445, DOI: 10.1002/prot.24133
KURSCHUS FC, FELLOWS E, STEGMANN E, JENNE DE: "Granzyme B delivery via perforin is restricted by size, but not by heparan sulfate-dependent endocytosis", PROC NATL ACAD SCI USA., vol. 105, no. 37, 16 September 2008 (2008-09-16), pages 13799 - 13804, XP009106108, DOI: 10.1073/pnas.0801724105
SINGLETON ET AL.: "Dictionary of Microbiology and Molecular Biology", 1994, J. WILEY & SONS
MARCH: "Mechanisms and Structure", vol. Advanced Organic Chemistry Reactions, 1992, JOHN WILEY & SONS
KINDT ET AL.: "Kuby Immunology", 2007, W.H. FREEMAN AND CO, pages: 91
PORTOLANO ET AL., J. IMMUNOL., vol. 150, 1993, pages 880 - 887
CLARKSON ET AL., NATURE, vol. 352, 1991, pages 624 - 628
J VIS EXP, no. 100, 10 June 2015 (2015-06-10), pages e52911
PROTEIN SCIENCE, vol. 4, 1995, pages 2411 - 2423
JOHNSON ET AL., BLOOD, vol. 114, 2009, pages 535 - 546
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