IMPROVED LUCIFERASE-BASED ASSAYS

WIPO Patent Application WO2004059294

A3

A method and kit is provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minize interference by at least about 10% relative to an assay not having tolerance enhancement agent.

WO/2019/036769	BRET SENSOR MOLECULES FOR DETECTING HYDROLASES
JP3983023	Method for enhancing luciferase luminescence reaction
WO/2017/037875	METHOD FOR ACQUIRING 3-DIMENSIONAL IMAGE, METHOD FOR DISPLAYING 3-DIMENSIONAL IMAGE, AND DEVICE FOR ACQUIRING 3-DIMENSIONAL IMAGE

HAWKINS ERIKA (US)
CALI JAMES J (US)
HO SAMUEL KIN SANG (US)
O'BRIEN MARTHA (US)
SOMBERG RICHARD (US)
BULLEIT ROBERT F (US)
WOOD KEITH V (US)

PCT/US2003/041454

June 29, 2006

December 23, 2003

Click for automatic bibliography generation  

PROMEGA CORP (US)
HAWKINS ERIKA (US)
CALI JAMES J (US)
HO SAMUEL KIN SANG (US)
O'BRIEN MARTHA (US)
SOMBERG RICHARD (US)
BULLEIT ROBERT F (US)
WOOD KEITH V (US)

C12Q1/66; C12Q1/68

US5744320A	1998-04-28
US5618682A	1997-04-08

HART R C ET AL: "Renilla reniformis Bioluminescence: Luciferase-Catalyzed Production of Non-radiating Excited States from Luciferin Analogues and Elucidation of the Excited State Species Involved in Energy Transfer to Renilla Green Fluorescent Protein.", BIOCHEMISTRY., vol. 18, no. 11, 1979, pages 2204 - 2210, XP003016321
See also references of EP 1588143A4

View/Download PDF      PDF Help

Previous Patent: MARKERS FOR DIFFERENTIAL DIAGNOSIS AND METHODS OF USE THEREOF

Next Patent: DEVICE FOR IDENTIFYING TYPES OF SHEET MATERIALS