Title:
IMPROVED METHODS FOR REPROGRAMING NON-PLURIPOTENT CELLS INTO PLURIPOTENT STEM CELLS
Document Type and Number:
WIPO Patent Application WO/2017/091943
Kind Code:
A1
Abstract:
Provided are chemical inducers of pluripotency (CIP) which include glycogen synthase kinase inhibitors, TGFβreceptor inhibitors, cyclic AMP agonists and S-adenosylhomocysteine hydrolase (SAH) inhibitors or histone acetylators. A method of inducing pluripotency in a partially or completely differentiated cell by using such chemical inducers of pluripotency is also provided. The method includes: (i) contacting a cell with the CIPs for a sufficient period of time to result in reprogramming the cell into a pluripotent stem cell having ESC-like characteristics (CiPSC). Isolated chemically induced pluripotent stem cells (CiPSCs) and their progeny, produced by inducing differentiation of the CiPSCs, can be used in a number of applications, including but not limited to cell therapy and tissue engineering
Inventors:
DENG HONGKUI (CN)
ZHAO YANG (CN)
ZHAO TING (CN)
GUAN JINGYANG (CN)
ZHANG XU (CN)
FU YAO (CN)
YE JUNQING (CN)
ZHAO YANG (CN)
ZHAO TING (CN)
GUAN JINGYANG (CN)
ZHANG XU (CN)
FU YAO (CN)
YE JUNQING (CN)
Application Number:
PCT/CN2015/095981
Publication Date:
June 08, 2017
Filing Date:
November 30, 2015
Export Citation:
Assignee:
HONG GUAN LTD (CN)
International Classes:
C12N5/071
Foreign References:
| CN104278008A | 2015-01-14 | |||
| CN105039258A | 2015-11-11 | |||
| CN104673741A | 2015-06-03 | |||
| CN103380212A | 2013-10-30 | |||
| CN2014081961W | 2014-07-10 | |||
| US8637494B2 | 2014-01-28 | |||
| US8236299B2 | 2012-08-07 | |||
| US6962814B2 | 2005-11-08 | |||
| US7914579B2 | 2011-03-29 | |||
| US5716404A | 1998-02-10 | |||
| US8728495B2 | 2014-05-20 | |||
| US20090029322A1 | 2009-01-29 | |||
| US20060019326A1 | 2006-01-26 | |||
| US20070059293A1 | 2007-03-15 |
Other References:
WILMUT ET AL., NATURE, vol. 385, 1997, pages 810 - 813
TAKAHASHIYAMANAKA, CELL, vol. 126, 2006, pages 663 - 676
YAMANAKA ET AL., NATURE, vol. 465, 2010, pages 704 - 712
STADTFELD ET AL., GENES DEV., vol. 24, 2010, pages 2239 - 2263
ZHU, ANNU. REV. BIOMED. ENG., vol. 13, 2011, pages 73 - 90
LI ET AL., CELL RES., vol. 21, 2011, pages 196 - 204
LI ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 109, 2012, pages 20853 - 20858
SAHA ET AL., CELL STEM CELL, vol. 5, 2009, pages 584 - 595
WU ET AL., CELL BIOL., vol. 13, 2011, pages 497 - 505
LEE ET AL., CHEM AND BIOL., vol. 13, 2006, pages 563 - 567
ZHOU ET AL., CHEM BIOI. AND DRUG DESIGN, vol. 85, no. 6, 2015, pages 659 - 671
SCHMIDTTT ET AL., J. MED. CHEM., vol. 56, no. 18, 2013, pages 7334 - 7342
BALLADUR ET AL., SURGERY, vol. 117, 1995, pages 189 - 94
DIXIT ET AL., CELL TRANSPLANTATION, vol. 1, 1992, pages 275 - 79
SHAPIRO ET AL., N ENGL J MED, vol. 343, no. 4, 2000, pages 230 - 8
WILLERTH, S.M.SAKIYAMA-ELBERT, S.E.: "StemBook", THE STEM CELL RESEARCH COMMUNITY, article "Combining stem cells and biomaterial scaffolds for constructing tissues and cell delivery"
HOCHEDLINGER ET AL., CELL, vol. 122, 2005, pages 947 - 956
LICHTI ET AL., NAT. PROTOC., vol. 3, 2008, pages 799 - 810
SELUANOV ET AL., J. VIS. EXP., vol. 2010, 2010, pages 2033
TAT ET AL., CELL TRANSPLANT, vol. 19, 2010, pages 525 - 536
MCQUALTER ET AL., STEM CELLS, vol. 27, 2009, pages 623 - 633
MAHERALI ET AL., CELL STEM CELL, vol. 3, 2008, pages 543 - 554
HOU ET AL., SCIENCE, vol. 341, 2013, pages 651 - 654
LONGO ET AL., TRANSGENIC RES, vol. 6, 1997, pages 321 - 328
SAMAVARCHI-TEHRANI ET AL., CELL STEM CELL, vol. 7, 2010, pages 64 - 77
ZHANG ET AL., CELL RES, vol. 21, 2011, pages 196 - 204
SILVA ET AL., PLOSBIOL, vol. 6, 2008, pages e253
THEUNISSEN ET AL., CURR. BIOL., vol. 21, 2011, pages 65 - 71
MIKKELSEN ET AL., NATURE, vol. 454, 2008, pages 519 - 523
MAHERALI ET AL., CURR. BIOL., vol. 19, 2009, pages 1718 - 1723
INSEL ET AL., CELL. MOL. NEUROBIOL., vol. 23, 2003, pages 305 - 314
CHIANG ET AL., PHARMACOL., THER., vol. 77, 1998, pages 115 - 134
GORDON ET AL., EUR. J. BIOCHEM., vol. 270, 2003, pages 3507 - 3517
NAKAGAWA ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 107, 2010, pages 14152 - 14157
SHU ET AL., CELL, vol. 153, 2013, pages 963 - 975
NEFF, DEV. DYN., vol. 240, 2011, pages 979 - 989
CHIANG ET AL., PHARMACOL. THER., vol. 77, 1998, pages 115 - 134
FELDMAN ET AL., NAT. CELL BIOL., vol. 8, 2006, pages 188 - 194
CHEN, NAT. GENET., vol. 45, 2013, pages 34 - 42
SILVA ET AL., PLOS BIOL, vol. 6, 2008, pages e253
CHAZAUD, DEV. CELL, vol. 10, 2006, pages 615 - 624
LIM ET AL., CELL STEM CELL., vol. 3, 2008, pages 543 - 554
MCDONALD ET AL., CELL REPORTS, vol. 9, 2014, pages 780 - 793
BUGANIM ET AL., CELL, vol. 151, 2012, pages 1617 - 1632
BUGANIM ET AL., CELL STEM CELL, vol. 15, 2014, pages 295 - 309
TAKAHASHI ET AL., CELL, vol. 131, 2007, pages 861 - 872
TAKAHASHI ET AL., NATURE COMMUNICATIONS, vol. 5, 2014, pages 3678
GOLIPOUR ET AL., CELL STEM CELL, vol. 11, 2012, pages 769 - 782
SRIDHARAN ET AL., CELL, vol. 136, 2009, pages 364 - 377
KUNATH ET AL., DEVELOPMENT, vol. 132, 2005, pages 1649 - 1661
CACCHIARELLI ET AL., CELL, vol. 162, 2015, pages 412 - 424
XENOPOULOS ET AL., CELL REPORTS, vol. 10, 2015, pages 1508 - 1520
AKSOY ET AL., THE EMBO JOURNAL, vol. 32, 2013, pages 938 - 953
CHAZAUD ET AL., DEVELOPMENTAL CELL, vol. 10, 2006, pages 615 - 624
NIAKAN ET AL., GENES & DEVELOPMENT, vol. 24, 2010, pages 312 - 326
YAMANAKA ET AL., DEVELOPMENT, vol. 137, 2010, pages 715 - 724
See also references of EP 3384007A4
TAKAHASHIYAMANAKA, CELL, vol. 126, 2006, pages 663 - 676
YAMANAKA ET AL., NATURE, vol. 465, 2010, pages 704 - 712
STADTFELD ET AL., GENES DEV., vol. 24, 2010, pages 2239 - 2263
ZHU, ANNU. REV. BIOMED. ENG., vol. 13, 2011, pages 73 - 90
LI ET AL., CELL RES., vol. 21, 2011, pages 196 - 204
LI ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 109, 2012, pages 20853 - 20858
SAHA ET AL., CELL STEM CELL, vol. 5, 2009, pages 584 - 595
WU ET AL., CELL BIOL., vol. 13, 2011, pages 497 - 505
LEE ET AL., CHEM AND BIOL., vol. 13, 2006, pages 563 - 567
ZHOU ET AL., CHEM BIOI. AND DRUG DESIGN, vol. 85, no. 6, 2015, pages 659 - 671
SCHMIDTTT ET AL., J. MED. CHEM., vol. 56, no. 18, 2013, pages 7334 - 7342
BALLADUR ET AL., SURGERY, vol. 117, 1995, pages 189 - 94
DIXIT ET AL., CELL TRANSPLANTATION, vol. 1, 1992, pages 275 - 79
SHAPIRO ET AL., N ENGL J MED, vol. 343, no. 4, 2000, pages 230 - 8
WILLERTH, S.M.SAKIYAMA-ELBERT, S.E.: "StemBook", THE STEM CELL RESEARCH COMMUNITY, article "Combining stem cells and biomaterial scaffolds for constructing tissues and cell delivery"
HOCHEDLINGER ET AL., CELL, vol. 122, 2005, pages 947 - 956
LICHTI ET AL., NAT. PROTOC., vol. 3, 2008, pages 799 - 810
SELUANOV ET AL., J. VIS. EXP., vol. 2010, 2010, pages 2033
TAT ET AL., CELL TRANSPLANT, vol. 19, 2010, pages 525 - 536
MCQUALTER ET AL., STEM CELLS, vol. 27, 2009, pages 623 - 633
MAHERALI ET AL., CELL STEM CELL, vol. 3, 2008, pages 543 - 554
HOU ET AL., SCIENCE, vol. 341, 2013, pages 651 - 654
LONGO ET AL., TRANSGENIC RES, vol. 6, 1997, pages 321 - 328
SAMAVARCHI-TEHRANI ET AL., CELL STEM CELL, vol. 7, 2010, pages 64 - 77
ZHANG ET AL., CELL RES, vol. 21, 2011, pages 196 - 204
SILVA ET AL., PLOSBIOL, vol. 6, 2008, pages e253
THEUNISSEN ET AL., CURR. BIOL., vol. 21, 2011, pages 65 - 71
MIKKELSEN ET AL., NATURE, vol. 454, 2008, pages 519 - 523
MAHERALI ET AL., CURR. BIOL., vol. 19, 2009, pages 1718 - 1723
INSEL ET AL., CELL. MOL. NEUROBIOL., vol. 23, 2003, pages 305 - 314
CHIANG ET AL., PHARMACOL., THER., vol. 77, 1998, pages 115 - 134
GORDON ET AL., EUR. J. BIOCHEM., vol. 270, 2003, pages 3507 - 3517
NAKAGAWA ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 107, 2010, pages 14152 - 14157
SHU ET AL., CELL, vol. 153, 2013, pages 963 - 975
NEFF, DEV. DYN., vol. 240, 2011, pages 979 - 989
CHIANG ET AL., PHARMACOL. THER., vol. 77, 1998, pages 115 - 134
FELDMAN ET AL., NAT. CELL BIOL., vol. 8, 2006, pages 188 - 194
CHEN, NAT. GENET., vol. 45, 2013, pages 34 - 42
SILVA ET AL., PLOS BIOL, vol. 6, 2008, pages e253
CHAZAUD, DEV. CELL, vol. 10, 2006, pages 615 - 624
LIM ET AL., CELL STEM CELL., vol. 3, 2008, pages 543 - 554
MCDONALD ET AL., CELL REPORTS, vol. 9, 2014, pages 780 - 793
BUGANIM ET AL., CELL, vol. 151, 2012, pages 1617 - 1632
BUGANIM ET AL., CELL STEM CELL, vol. 15, 2014, pages 295 - 309
TAKAHASHI ET AL., CELL, vol. 131, 2007, pages 861 - 872
TAKAHASHI ET AL., NATURE COMMUNICATIONS, vol. 5, 2014, pages 3678
GOLIPOUR ET AL., CELL STEM CELL, vol. 11, 2012, pages 769 - 782
SRIDHARAN ET AL., CELL, vol. 136, 2009, pages 364 - 377
KUNATH ET AL., DEVELOPMENT, vol. 132, 2005, pages 1649 - 1661
CACCHIARELLI ET AL., CELL, vol. 162, 2015, pages 412 - 424
XENOPOULOS ET AL., CELL REPORTS, vol. 10, 2015, pages 1508 - 1520
AKSOY ET AL., THE EMBO JOURNAL, vol. 32, 2013, pages 938 - 953
CHAZAUD ET AL., DEVELOPMENTAL CELL, vol. 10, 2006, pages 615 - 624
NIAKAN ET AL., GENES & DEVELOPMENT, vol. 24, 2010, pages 312 - 326
YAMANAKA ET AL., DEVELOPMENT, vol. 137, 2010, pages 715 - 724
See also references of EP 3384007A4
Attorney, Agent or Firm:
CHINA SCIENCE PATENT & TRADEMARK AGENT LTD. (CN)
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