Field of the invention

The present invention relates to cost effective and industrially advantageous process for the preparation of (3R,4S)-l-(4-Fluorophenyl)-3-[3(S)-3-(4-fluorophenyl)-3 - hydroxypropyl)]-4-(4-hydroxyphenyl)-2-azetidinone, referred to here as Ezetimibe. It is represented as Formula- 1.

Formula-l

Background of the invention

This invention relates to an improved, cost effective and industrially advantageous process for the preparation of (3R, 4S)-l-(4-Fluorophenyl)-3-[3(S)-3-(4- fluorophenyl)-3 -hydroxypropyl)]-4-(4-hydroxyphenyl)-2-azetidinone (Ezetimibe), useful as cholesterol absorption inhibitor, claimed in U.S.Pat. No. 5,767,115. Preparation of Ezetimibe is also described in the above said patent. It comprises of (S)-4- phenyl-2- oxazolidinone is reacted with methyl-4-(chloroformyl)butyrate to obtain a compound of ester and it is condensed with 4-benzyloxy benzylidine (4-fluoro) aniline in the presence of titanium isopropoxide and titanium tetrachloride to give an amide compound and it is cyclised in the presence of tetrabutyl ammonium fluoride and bis trim ethyl silyl acetamide to give protected lactam, it undergoes hydrolysis to give a carboxylic acid and further it reacts with p- fluoro phenyl magnesium bromide and zinc chloride in the

presence of tetrakis ( triphenyl phosphine) palladium to give an aromatic ketone, it is further reduced selectively in the presence of chiral catalyst to obtain an hydroxy compound and it undergoes debenzylation to give the title compound of formula-I.

By following the above process in the hydrolysis stage after completion of the reaction, pH adjusted to acidic side ( below pH- 4) and extracting the compound resulted more impure product because as the Lactam ring is acid sensitive, it is opened up while adjusting the pH to below 4. In the organometalic condensation reaction between acid chloride and para fluoro phenyl znic halide, tetrakis ( triphenyl phosphine) palladium is used as a catalyst which is highly expensive as well as molecular weight is higher which resulted in more byproducts and obtained compound is less pure.

The above said process is both uneconomical, inconsistency in reproducibility and more of byproduct/waste generation like triphenyl phosphine oxide, DMS, etc., and hence it is not suitable for commercial production.

Therefore, the main objective of the present invention is to prepare formula-1 through a process which is cost-effective, commercially viable, eco-friendly and consistent.

The formula-1 is prepared in the present invention, in a novel process that is cost effective and suitable for commercial scale up.

Summary of the invention

This invention provides an improved simple and cost effective, eco-friendly and well suited for commercial scale up of (3R,4S)-l-(4-Fluorophenyl)-3-[3(S)-3-(4- fluorophenyl)-3 -hydroxypropyl)]-4-(4-hydroxyphenyl)-2-azetidinone, referred to here as Ezetimibe. It is represented as Formula- 1.

Formula-1

Comprising:

(a) Glutaric anhydride of formula-II reacting with an aliphatic or alicyclic alcohol in Presence of a base to give ester compound of formula-Ill.

alkyl, Cl-Cδ cyclo alkyl

Formula-II

(b). Using pivalyl chloride in the preparation of compound having the structural formula- V, by reacting it with compound of formula-Ill in presence of a acid trapping agent and subsequent reaction with chiral auxiliary of formula-IV

Formula- V

(c) Reacting the ketone of Formula- V with benzylated imine of formula- VI in presence of Lewis acid to obtain an amide of formula- VII.

Formula - VI Formula-V Formula -VII

(d) Cyclizing the amide of formulaVII with

(i) a silylationg agent and a fluoride ion catalyst as a cyclizating agent.

(ii) A strong non-nucleophilic base to obtain the compound of formula- VIII.

Formula-VIII

(e). Hydrolising the ester group in formula VIII with a base in a suitable solvent to give the carboxylic acid compound of formula-IX. Adjusting the pH to 5 - 7 of hydrolysis reaction mass, preferably 6.5 to 7.0 and extraction of carboxylic acid compound of formula-IX.

h

Formula-IX

(f). Compound of formula-IX is converted into acid chloride and further it is converted into formula-X by organo metallic reaction in presence of a Palladium acetate as catalyst.

Formula- IX Formula- X

(g). Reducing the aromatic ketone of formula-X in the presence of a chiral reducing agent or reducing agent in presence of a chiral catalyst to obtain hydroxy compound of formula-XL

Formula-XI

(h). Debenzylation of the compound of formula-XI with Pd/C to give the compound of formula-I.

Formula-I

Detailed description of the invention

The present invention relates an improved process for the preparation of (3R,4S)-l-(4-Fluorophenyl)-3-[3(S)-3-(4-fluorophenyl)-3 -hydroxypropyl)]-4-(4- hydroxyphenyl)-2-azetidinone, referred to here as (Ezetimibe).

The process of the present invention is schematically represented as follows.

Comprising:

a. Reacting Glutaric anhydride of structural formula-II with an aliphatic alcohol or alicyclic alcohol in presence of a base sodium methoxide to give compound of formula-Ill. b. Reacting the formula-Ill with pivalyl chloride in presence of a acid trapping agent like triethyl amine, diisopropyl ethyl amine or inorganic bases like sodium carbonate, potassium carbonate and further it is reacting with a chiral auxiliary of formula-IV to give ketone compound of structural formula of V. c. Ketone compound of formula-V is further condensed with an benzylated imine of formula- VI to give the an amide compound of formula- VII in presence of a Lewis acid. d. Cyclising the amide of formula- VII with a silylating agent and a fluoride ion catalyst as a cyclising agent to give the protected lactam compound of formula- VIII. e. Hydrolysis of protected lactam compound of formula- VIII with a base to give the carboxylic acid of formula-IX. f. Carboxylic acid of formula-IX is converts into acid chloride with oxalyl chloride and it is further condensed with para bromo fluoro benzene through organo metallic reaction to give the aromatic ketone of formula-X. g. Reducing the ketone of formula-X with chiral reducing agent or in the presence of a chiral catalyst to give chiral alcohol of formula-XI. h. Debenzylation of formula-XI with Pd/C to give the compound of formula-I.

Step-a: The reaction is carried out at a temperature -20°C to 65°C, preferably at 25- 35°C for 30 minutes to 15 hrs, preferably 6-8 hrs. Step-b: pivalyl chloride reaction is carried out in a suitable solvent like dichloromethane, toluene, xylene, preferably in dichloromethane, in presence of a acid trapping agent such as triethyl amine, disiopropyl amine or inoganic base like NaHCO ₃ , Na ₂ CO ₃ , K ₂ CO _3j preferably triethyl amine. Reaction is conducted at a temperature of- 10°C to 50°C preferably at 15-30°C for 1 to 10 hrs, preferably for 2-4 hrs.

Chiral auxiliary such as the compound of formula-IV is used like S-POZ, S- BOZ, preferably S-POZ. This reaction is carried out inpresence of dimethyl amino pyridine as a catalyst at the temperature of 10-50°C , preferably at 40-50°C for 30 minutes to 10 hrs, preferably 4-6 hrs.

Step-c: Condensation reaction of step-c can be carried out in a suitable solvent like dichloromethane, toluene, xylene, preferably dichloromethane and treated with a Lewis acid such as TiCl ₄ at about -60 to 0 ⁰ C, preferably at about -25°C , under a dry, inert atmosphere like nitrogen or orgon. A tert. amine base such as diisopropyl ethyl amine is used as a trapping agent. This reaction is conducted for 3-6 hrs at -25 to -10 ⁰ C , preferably about 4 hrs or until reaction is completely by TLC.

Step-d: The cyclisation of step-d can be carried out in a suitable solvent like dichloro methane, toluene, xylene, ethyl acetate, preferably in toluene at a temperature 0 ⁰ C to 80 ⁰ C, preferably at 40-50 ⁰ C with silylating agent like bis trimethyl silyl acetamide and a cyclising agent like tetra butyl ammonium fluoride.

Step-e: Hydrolysis of formula VIII with a suitable solvent like an alcohol solvent like ethanol, isopropyl alcohol, tert-butanol or ketonic solvents like acetone, methyl isobutyl ketone, preferably in acetone with a base like alkali and alkaline earth metal hydroxides, preferably sodium hydroxide at a temperature 0 ⁰ C to 50 ⁰ C, preferably at 25-35 ⁰ C for 30 minutes to 10 hrs, preferably 2-3 hrs to obtain a carboxylic acid of formula IX. Adjusting the pH to 5 - 7 of reaction mass, preferably 6.5 to 7.0 and extraction of carboxylic acid compound.

Step-f : Converting the Carboxylic acid of formula-IX into acid chloride using acid halogenating agent like, oxalyl chloride, thionyl chloride and phosphorous halides, preferably with oxalyl chloride and with a suitable solvent like dichloromethane, toluene, xylene or ethyl acetate, preferably dichloromethane. This reaction is carried out an inert atmosphere like nitrogen at 0-40°C for 10 minutes 5hrs. Preferably at 25-35°C for 1-3 hrs

Acid chloride is further condensed with a Grignard reagent ( it is prepared from the reaction of para bromo fluoro benzene with magnesium turnings to form -4 fluoro phenyl magnesium bromide and it is further converted to 4-fluoro phenyl zinc halide with anhydrous zinc chloride) in presence of a transition metal catalyst namely palladium acetate to give the condensed product as formula X. This reaction is carried out with a suitable solvent like dichloromethane, toluene, xylene or ethyl acetate, preferably toluene. This reaction is carried out in an inert atmosphere like nitrogen at -10 to 40°C for 10 minutes to 3.00 hrs. Preferably at 10-15°C for 30- 60 minutes.

Step-g: Reduction of ketone of formula-X to hydroxy group using a chiral reducing agent such as DIP Chloride or reducing agent Borane THF or Borane-DMS, in the presence of a chiral catalyst such as (R)-tetrahydro-l-methyl-3,3-diphenyl-l H,3H-pyrrol (l,2-c)(l,3,2) oxaza - borolidine (R-Methyl CBS) or R- Butyl CBS or R-Phenyl CBS This reaction is carried out at with a suitable solvent like dichloromethane, toluene, xylene or ethyl acetate, preferably toluene. This reaction is carried out an inert atmosphere like nitrogen at -10 to 4O ⁰ C for 30 minutes 10 hrs. Preferably at 0 to 5 ⁰ C for 2-3 hrs.

Step-h: Debenzylation of formula-XI with palladium carbon to give the title compound of formula-I. This reaction is is carried out at with a suitable solvent like an alcoholic solvents like methanol, IPA, tert- butanol or dichloromethane, toluene preferably in IPA. This reaction is carried out at 10 to 70 ⁰ C for 30 minutes 10 hrs. Preferably at 45 to 50 ⁰ C for 2-3 hrs.

The examples mentioned below demonstrate specific preparations of the present invention. The examples are provided to illustrate the details of the invention and should not be constrained to limit the scope of the present invention.

EXAMPLE: 1

Preparation of (3R,4S)-l-(4-Fluorophenyl)-3-[3(S)-3-(4-fluorophenyl)-3 - hydroxypropyl)]-4-(4-hydroxyphenyl)-2-azetidinone, referred to here as Formula-1.

Step-a: Preparation of Monomethyl glutarate (formula-Ill)

Taken 100 gm of glutaric anhydride of formula-II and methanol (250 ml) and sodium methoxide (1 gm) in a 1 lit round bottom flask and stirred for 6 hrs at 25-35 ⁰ C. Distilled the solvent completely under vacuum at below 60°C. Residue is taken to next stage directly without any purification. Yield: 125 gm

Step-b: Preparation of ketone compound of formula- V

Formula -III material (45 gm) is dissolved in dichloromethane (225 ml) in 1 lit cleaned RBF. Added triethyl amine to the reaction mixture and stirred for 10 minutes. Pivaloyl chloride is added to the reaction mass in 45 minutes at 25-35°C. Maintained the reaction mass for 2 hrs. Added S-POZ (formula-IV), DMAP and DMF to the refluxed the reaction mixture for 7 hrs. Reaction mixture is quenched with water and separated the organic layer. Organic layer washed with water (100 ml) and distilled the solvent completely under vacuum and recrystallised the material in pet-ether. Yield: 50 gm.

Step-c: Preparation of compound of formula- VII.

Titanium tetrachloride (18 ml) and dichloromethane (600ml) were taken into a clean RBF, cooled the reaction mixture to 0°C and added titanium isopropoxide (16 ml) at 0-5°C. Dissolved the Formula-V material (50 gm) in dichloromethane and added to the reaction mass at 0-5°C and maintained the reaction mass for 15 minutes. Diisopropyl ethyl amine (65 ml) is added to the reaction mixture and maintained for 45 minutes and cooled to -20 to -10°C and charged the benzylated imine (92 gm) of formula- VI to the reaction mixture, maintained for 4 hrs at -20 to -1O ⁰ C. Quenched the reaction with acetic acid and washed the organic layer with sulfuric acid solution , distilled the solvent completely and material is recrystalised in methanol. Yield: 65 gm.

Step-d: Preparation of compound of formula- VIII (Cyclisation)

Taken toluene (100 ml) and compound of formula VII (25 gm) into cleaned RB.Flask, heated to 45°C and added N,O-bis trimethyl silyl acetamide( 17 gm) and TBAF.3 H ₂ O (1.1 gm) and maintained for 2 hrs at 45-5O ⁰ C. Reaction mass is quenched with methanol and washed with 1 N HcI solution , IN sodium bicarbonate solution followed by saturated sodium chloride solution, distilled the solvent completely under vacuum and toluene is charged and isolated Rec S-POZ, filtrate is concentrated and product is isolated in methanol and dried the product at 50-60 ⁰ C. ( Yield: 13 gm). M.R 60-65 ⁰ C.

Step-e: Preparation of compound of formula-IX.

Compound of formula- VIII (25 gm) is dissolved in acetone (25ml) in a cleaned R.B.Flask. Water (62.5 ml) and sodium hydroxide (2.8 gm) added to the reaction mass. Maintained the reaction mass for 3 hrs at ambient temperature. Reaction mass quenched with water and pH adjusted to 6.5-7.0 with aqueous hydrochloric acid. Reaction mass extracted with ethyl acetate and distilled the solvent completely under vacuum to give residue of the compound. It is used into next stage without any purification. Yield: 22 gm.

Step-f: Preparation of compound of formula-X.

Compound of formula-IX (25gm) is dissolved in dichloromethane in a R.B.Flask. and catalytical amount of Dimethyl formamide is added. Oxalyl chloride is slowly added at ambient temperature, maintained the reaction mass for 3 hrs at ambient temperature. Distilled the solvent completely under vacuum and toluene is added and again distilled completely under vacuum. Toluene is added to the crude and cooled to 10-15°C and then the palladium acetate and maintained for 15 minutes.

4-fluorophenyl magnesium bromide is taken in another vessel under nitrogen atmosphere and cooled to 0 -5°C and added anhydrous zinc chloride and stirred for 1 hr. This complex is added to the acid chloride reaction mass at ambient temperature and maintained for 45 minutes. Filtered the reaction mass through hyflow and washed with toluene and THF mixed solution. Distilled the solvent completely under vacuum, added dichloromethane and silica gel to the crude and distilled the solvent completely under vacuum, Cyclohexane is added to the silicagel mixture and stirred for 30 minutes and . filtered and washed with cyclohexane. Silicagel mixture is slurried with ethyl acetate and cyclohexane. Distilled both filtrates under vacuum to get the residue. (Yield: 18 gm). Step-g: Exaniple-1: Preparation of compound of hydroxy compound of formula-XL

Taken toluene (250 ml) into cleaned R.B.Flask under nitrogen atmosphere and cooled to 0-5°C. Borane DMS complex and (R)-tetrahydro-l-phenyl-3,3-diphenyl-l H,3H-pyrrol (l,2-c)(l,3,2) oxaza borolidine (R-phenyl CBS) is charged into the reaction mass at 0°C. 25 gm of Keto compound of formula-X is dissolved in toluene(50 ml) and added to the reaction mass at 0-5°C. Maintained the reaction mass for 3 hrs and quenched with methanol and followed by 1 N hydrochloric acid solution. Organic layer separated and washed with 5% hydrogen peroxide solution and 5% sodium sulfate solution and followed by with 10% sodium chloride solution. Distilled the solvent completely under reduced pressure at below 75°C. Product is isolated in diisopropyl ether and dried the product at 60-70°C for 6 hrs. (Yield: 15 gm).

Example-2: Preparation of compound of hydroxy compound of formula-XL

Taken toluene (250 nil) into cleaned R.B.Flask under nitrogen atmosphere and cooled to 0-5°C. DIP Chloride (Mole ratio 1:1.5) into the reaction mass at O ⁰ C. 25 gm of keto compound of formula-X is dissolved in toluene(50 ml) and added to the reaction mass at 0-5 ⁰ C. Maintained the reaction mass for 3 hrs and quenched with ammonia solution. Organic layer separated and washed with 10% sodium chloride solution. Distilled the solvent completely under reduced pressure at below 75 ⁰ C. Residue is taken for next stage directly without any purification.

Step-h: Preparation of compound of formula-I (Ezetimibe).

Taken compound of formula-XII (10 gm) and isopropanol (100 ml) into a hydrogenation flask, added 5 % Pd/C ( 4gm) at 25°C and maintained at 45-50 ⁰ C for 3 hrs under hydrogen pressure, filtered through hyflow and washed the Pd/C with isopropanol(20 ml). Distilled the solvent completely under vacuum at below 7O ⁰ C, product is recrystallised in dichloromethane (Yield: 6 gm).

Purification of Ezetimibe (formula-1).

Ezetimibe (10 gm) is dissolved in 30 ml of methanol and filtered through hyflow and saturated with DM.Water(30 ml) and stirred for 1 hr at 20-25 ⁰ C. Product filtered and dried for 6-8 hrs at 80-85 ⁰ C (Yield:9 gm):