Title:
IMPROVEMENT OF GLUCOSE DEHYDROGENASE
Document Type and Number:
WIPO Patent Application WO/2019/230614
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a flavin adenine dinucleotide-dependent glucose dehydrogenase having further improved substrate specificity and/or low-temperature reactivity and having higher practically value. Disclosed is a flavin adenine dinucleotide-dependent glucose dehydrogenase having reduced xylose reactivity, which is produced utilizing a circular permutation (CP) mutation.
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Inventors:
ISHIHARA SATORU (JP)
NISHIO KYOICHI (JP)
LUTZ STEFAN (US)
NISHIO KYOICHI (JP)
LUTZ STEFAN (US)
Application Number:
PCT/JP2019/020777
Publication Date:
December 05, 2019
Filing Date:
May 24, 2019
Export Citation:
Assignee:
AMANO ENZYME INC (JP)
International Classes:
C12N15/53; C12M1/40; C12N1/15; C12N1/19; C12N1/21; C12N9/04; C12N15/09; C12N15/52; C12N15/63; C12Q1/32; C07K5/10; C07K7/06; C07K7/08
Domestic Patent References:
| WO2018062103A1 | 2018-04-05 | |||
| WO2007116710A1 | 2007-10-18 | |||
| WO2017077924A1 | 2017-05-11 |
Foreign References:
| JP2008529530A | 2008-08-07 | |||
| JP2012029677A | 2012-02-16 |
Other References:
GUNTAS, G. ET AL.: "Circular Permutation in the omega-Loop of TEM-1 beta-Lactamase Results in improved Activity and Altered Substrate Specificy", PLOS ONE, vol. 7, no. 4, 19 April 2012 (2012-04-19), pages e35998- 1 - 5, XP055662882
DAUGHERTY, A. B. ET AL.: "Improved Biocatalysists from a Synthetic Circular Permutation Library of the Flavin-Dependent Oxidoreductase Old Yellow Enzyme", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 135, no. 38, 29 August 2013 (2013-08-29) - 25 September 2013 (2013-09-25), pages 14425 - 14432, XP055662883
DAUGHERTY, A. B. ET AL.: "Structural and Functional Consequences of Circular Permutation on the Active Site of Old Yellow Enzyme", ACS CATALYSIS, vol. 5, no. 2, 9 December 2014 (2014-12-09) - 6 February 2015 (2015-02-06), pages 892 - 899, XP055662884
DAUGHERTY, A. B. ET AL.: "Improved Biocatalysists from a Synthetic Circular Permutation Library of the Flavin-Dependent Oxidoreductase Old Yellow Enzyme", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 135, no. 38, 29 August 2013 (2013-08-29) - 25 September 2013 (2013-09-25), pages 14425 - 14432, XP055662883
DAUGHERTY, A. B. ET AL.: "Structural and Functional Consequences of Circular Permutation on the Active Site of Old Yellow Enzyme", ACS CATALYSIS, vol. 5, no. 2, 9 December 2014 (2014-12-09) - 6 February 2015 (2015-02-06), pages 892 - 899, XP055662884
Attorney, Agent or Firm:
HAGINO Mikiharu (JP)
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