Title:
IMPROVEMENTS IN INDUCED HETERODUPLEX GENERATORS
Document Type and Number:
WIPO Patent Application WO2006038037
Kind Code:
A3
Abstract:
Heteroduplex band resolution is found to be improved by modifying the induced heteroduplex generator (IHG) molecule such that the identifier is spaced apart from the nucleotide position immediately adjacent to the site of the polymorphism which is to be genotyped. Unexpectedly, it is found that, when such modified IHG molecules are used, the resolution of the induced heteroduplexes is significantly improved.
Inventors:
TILLEY LOUISE ANNE (GB)
Application Number:
PCT/GB2005/003896
Publication Date:
June 15, 2006
Filing Date:
October 10, 2005
Export Citation:
Assignee:
IHG DIAGNOSTICS LTD (GB)
TILLEY LOUISE ANNE (GB)
TILLEY LOUISE ANNE (GB)
International Classes:
C12Q1/68
Domestic Patent References:
| WO1993019201A1 | 1993-09-30 |
Other References:
BELLI CAROLINA ET AL: "A single, multiplex analysis for all relevant activating NRAS gene mutations using heteroduplex generators.", BRITISH JOURNAL OF HAEMATOLOGY. AUG 2004, vol. 126, no. 4, August 2004 (2004-08-01), pages 602 - 605, XP002374778, ISSN: 0007-1048
BELLI CAROLINA ET AL: "Rapid detection of exon 1 NRAS gene mutations using universal heteroduplex generator technology.", HUMAN MUTATION. FEB 2003, vol. 21, no. 2, February 2003 (2003-02-01), pages 132 - 137, XP009064424, ISSN: 1098-1004
WOOD N ET AL: "RAPID CLASSIFICATION OF PHENYLKETONURIA GENOTYPES BY ANALYSIS OF HETERODUPLEXES GENERATED BY PCR-AMPLIFIABLE SYNTHETIC DNA", HUMAN MUTATION, WILEY-LISS, NEW YORK, NY, US, vol. 2, no. 2, January 1993 (1993-01-01), pages 131 - 137, XP000606266, ISSN: 1059-7794
CULPAN D ET AL: "Rapid mutation screening in type 2A von Willebrand's disease using universal heteroduplex generators.", BRITISH JOURNAL OF HAEMATOLOGY. MAR 1997, vol. 96, no. 3, March 1997 (1997-03-01), pages 464 - 469, XP002374779, ISSN: 0007-1048
MORSE H R ET AL: "INDUCED HETERODUPLEX GENOTYPING OF TNF-alpha, IL-1beta, IL-6 AND IL-10 POLYMORPHISMS ASSOCIATED WITH TRANSCRIPTIONAL REGULATION", CYTOKINE, ACADEMIC PRESS LTD, PHILADELPHIA, PA, US, vol. 11, no. 10, October 1999 (1999-10-01), pages 789 - 795, XP004721342, ISSN: 1043-4666
WANG Y H ET AL: "Effects of bulge composition and flanking sequence on the kinking of DNA by bulged bases.", BIOCHEMISTRY. 5 FEB 1991, vol. 30, no. 5, 5 February 1991 (1991-02-05), pages 1358 - 1363, XP002374781, ISSN: 0006-2960
LUEBKE K J ET AL: "Sequence effects on RNA bulge-induced helix bending and a conserved five-nucleotide bulge from the group I introns.", BIOCHEMISTRY. 10 SEP 1996, vol. 35, no. 36, 10 September 1996 (1996-09-10), pages 11677 - 11684, XP002374782, ISSN: 0006-2960
BHATTACHARYYA A ET AL: "The contrasting structures of mismatched DNA sequences containing looped-out bases (bulges) and multiple mismatches (bubbles).", NUCLEIC ACIDS RESEARCH. 12 SEP 1989, vol. 17, no. 17, 12 September 1989 (1989-09-12), pages 6821 - 6840, XP002374783, ISSN: 0305-1048
WOOD N ET AL: "Optimisation and properties of a UHG for genotyping of hemoglobins S and C.", HUMAN MUTATION. 1995, vol. 5, no. 2, 1995, pages 166 - 172, XP002374784, ISSN: 1059-7794
NATARAJ A J ET AL: "Single-strand conformation polymorphism and heteroduplex analysis for gel-based mutation detection.", ELECTROPHORESIS. JUN 1999, vol. 20, no. 6, June 1999 (1999-06-01), pages 1177 - 1185, XP002374785, ISSN: 0173-0835
BELLI CAROLINA ET AL: "Rapid detection of exon 1 NRAS gene mutations using universal heteroduplex generator technology.", HUMAN MUTATION. FEB 2003, vol. 21, no. 2, February 2003 (2003-02-01), pages 132 - 137, XP009064424, ISSN: 1098-1004
WOOD N ET AL: "RAPID CLASSIFICATION OF PHENYLKETONURIA GENOTYPES BY ANALYSIS OF HETERODUPLEXES GENERATED BY PCR-AMPLIFIABLE SYNTHETIC DNA", HUMAN MUTATION, WILEY-LISS, NEW YORK, NY, US, vol. 2, no. 2, January 1993 (1993-01-01), pages 131 - 137, XP000606266, ISSN: 1059-7794
CULPAN D ET AL: "Rapid mutation screening in type 2A von Willebrand's disease using universal heteroduplex generators.", BRITISH JOURNAL OF HAEMATOLOGY. MAR 1997, vol. 96, no. 3, March 1997 (1997-03-01), pages 464 - 469, XP002374779, ISSN: 0007-1048
MORSE H R ET AL: "INDUCED HETERODUPLEX GENOTYPING OF TNF-alpha, IL-1beta, IL-6 AND IL-10 POLYMORPHISMS ASSOCIATED WITH TRANSCRIPTIONAL REGULATION", CYTOKINE, ACADEMIC PRESS LTD, PHILADELPHIA, PA, US, vol. 11, no. 10, October 1999 (1999-10-01), pages 789 - 795, XP004721342, ISSN: 1043-4666
WANG Y H ET AL: "Effects of bulge composition and flanking sequence on the kinking of DNA by bulged bases.", BIOCHEMISTRY. 5 FEB 1991, vol. 30, no. 5, 5 February 1991 (1991-02-05), pages 1358 - 1363, XP002374781, ISSN: 0006-2960
LUEBKE K J ET AL: "Sequence effects on RNA bulge-induced helix bending and a conserved five-nucleotide bulge from the group I introns.", BIOCHEMISTRY. 10 SEP 1996, vol. 35, no. 36, 10 September 1996 (1996-09-10), pages 11677 - 11684, XP002374782, ISSN: 0006-2960
BHATTACHARYYA A ET AL: "The contrasting structures of mismatched DNA sequences containing looped-out bases (bulges) and multiple mismatches (bubbles).", NUCLEIC ACIDS RESEARCH. 12 SEP 1989, vol. 17, no. 17, 12 September 1989 (1989-09-12), pages 6821 - 6840, XP002374783, ISSN: 0305-1048
WOOD N ET AL: "Optimisation and properties of a UHG for genotyping of hemoglobins S and C.", HUMAN MUTATION. 1995, vol. 5, no. 2, 1995, pages 166 - 172, XP002374784, ISSN: 1059-7794
NATARAJ A J ET AL: "Single-strand conformation polymorphism and heteroduplex analysis for gel-based mutation detection.", ELECTROPHORESIS. JUN 1999, vol. 20, no. 6, June 1999 (1999-06-01), pages 1177 - 1185, XP002374785, ISSN: 0173-0835
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