Title:
IN SITU WHOLE GENOME CHROMATIN CONFORMATION CAPTURE METHOD FOR INFINITESIMAL CELLS
Document Type and Number:
WIPO Patent Application WO/2019/006975
Kind Code:
A1
Abstract:
Provided is a method for constructing a DNA sequencing library of a genome to be tested, the method comprising: (1) digesting a genome to be tested by means of a restriction enzyme; (2) performing biotin labelling on digested products; (3) linking the biotin-labelled products using a DNA ligase; (4) decrosslinking the linked products; (5) purifying the decrosslinked products; (6) performing ultrasonic and precipitation treatments on the purified products, wherein the precipitation treatment is to bring the sonicated products into contact with streptavidin magnetic beads to obtain target DNA fragments bound to streptavidin magnetic beads; and (7) constructing a library based on the target DNA fragments bound to streptavidin magnetic beads.
Inventors:
XIE WEI (CN)
DU ZHENHAI (CN)
DU ZHENHAI (CN)
Application Number:
PCT/CN2017/114475
Publication Date:
January 10, 2019
Filing Date:
December 04, 2017
Export Citation:
Assignee:
UNIV TSINGHUA (CN)
International Classes:
C40B50/06; C12Q1/68; C40B40/06
Foreign References:
| CN106566828A | 2017-04-19 | |||
| CN105754995A | 2016-07-13 | |||
| CN107217309A | 2017-09-29 | |||
| CN106637422A | 2017-05-10 |
Attorney, Agent or Firm:
TSINGYIHUA INTELLECTUAL PROPERTY LLC (CN)
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