Title:
IN-VITRO ENDOTHELIAL CELL CULTURE SYSTEM FOR OPTIMIZING PULSATILE WORKING MODES OF CONTINUOUS-FLOW ARTIFICIAL HEART
Document Type and Number:
WIPO Patent Application WO/2022/165863
Kind Code:
A1
Abstract:
An in-vitro endothelial cell culture system for optimizing pulsatile working modes of a continuous-flow artificial heart, relating to the technical field of artificial organs. The system comprises three parts: 1) a cell culture cavity on a microfluidic chip and an off-chip multi-component aortic arch afterload fluid mechanic circulation loop; 2) a device for simulating a cardiovascular system power source: a fluid loading device is implemented by a pulsatile blood pump, and an artificial heart device is connected in parallel across two ends of the pulsatile blood pump; and 3) a peripheral detection and feedback control system, comprising pressure and flow sensors, a fluorescence microscope, a high-speed CCD camera system, and a proportional-integral-derivative feedback control system. The system can accurately simulate real hemodynamic microenvironments of vascular endothelial cells at different parts of the aortic arch, and provides a miniature, objective, standard, and quantitative experimental platform for studying quantitative relationships between different pulsatile working modes of the pump speed of an artificial heart and hemodynamic signals in the local arterial endothelial microenvironment.
Inventors:
WANG YU (CN)
LIANG LIXUE (CN)
QIN KAIRONG (CN)
LI YONGJIANG (CN)
XUE CHUNDONG (CN)
WANG JUNWEI (CN)
NA JINGTONG (CN)
YANG YUNONG (CN)
LIANG LIXUE (CN)
QIN KAIRONG (CN)
LI YONGJIANG (CN)
XUE CHUNDONG (CN)
WANG JUNWEI (CN)
NA JINGTONG (CN)
YANG YUNONG (CN)
Application Number:
PCT/CN2021/077163
Publication Date:
August 11, 2022
Filing Date:
February 22, 2021
Export Citation:
Assignee:
UNIV DALIAN TECH (CN)
International Classes:
C12M3/00; C12M1/00; C12M1/34; C12M1/36
Foreign References:
| CN106754356A | 2017-05-31 | |||
| CN111426821A | 2020-07-17 | |||
| CN108977359A | 2018-12-11 | |||
| US20030143519A1 | 2003-07-31 |
Other References:
QIN, KAIRONG ET AL.: "State of the Art of the Methods and Techniques in Modeling Analysis and in Vitro Simulation of Arterial Endothelial Hemodynamic Microenvironment", JOURNAL OF EXPERIMENTS IN FLUID MECHANICS, vol. 34, no. 2, 30 April 2020 (2020-04-30), pages 11 - 24, XP055958237
SCOTT, JAMES H.: "Reactions of Substituted Phenacyl Bromides with Various Bases. o- and p- Nitrophenacyl Bromide. I", JOURNAL OF HETEROCYCLIC CHEMISTRY, vol. 21, no. 3, 1 January 1984 (1984-01-01), pages 903 - 904, XP055956822
CHANG HUN LEE, HO JOON SHIN, IN HEE CHO, YOUNG-MI KANG, IN AE KIM, KI-DONG PARK, JUNG-WOOG SHIN: "Nanofiber alignment and direction of mechanical strain affect the ECM production of human ACL fibroblast", BIOMATERIALS, vol. 26, no. 11, 1 April 2005 (2005-04-01), AMSTERDAM, NL , pages 1261 - 1270, XP055543637, ISSN: 0142-9612, DOI: 10.1016/j.biomaterials.2004.04.037
SCOTT, JAMES H.: "Reactions of Substituted Phenacyl Bromides with Various Bases. o- and p- Nitrophenacyl Bromide. I", JOURNAL OF HETEROCYCLIC CHEMISTRY, vol. 21, no. 3, 1 January 1984 (1984-01-01), pages 903 - 904, XP055956822
CHANG HUN LEE, HO JOON SHIN, IN HEE CHO, YOUNG-MI KANG, IN AE KIM, KI-DONG PARK, JUNG-WOOG SHIN: "Nanofiber alignment and direction of mechanical strain affect the ECM production of human ACL fibroblast", BIOMATERIALS, vol. 26, no. 11, 1 April 2005 (2005-04-01), AMSTERDAM, NL , pages 1261 - 1270, XP055543637, ISSN: 0142-9612, DOI: 10.1016/j.biomaterials.2004.04.037
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