Title:
IN VITRO POTENCY ASSAY FOR PROTEIN-BASED MENINGOCOCCAL VACCINES USING A PLURALITY OF MONOCLONAL ANTIBODIES AND ANTIGENS IMMOBILIZED TO COLOUR-CODED BEADS
Document Type and Number:
WIPO Patent Application WO/2023/067031
Kind Code:
A3
Abstract:
This invention provides a multiplex binding assay for analysing a meningococcal vaccine and assessing in vitro relative potency of the same (IVRP). The invention also provides monoclonal antibodies which bind to meningococcal proteins within the vaccine, and in particular monoclonal antibodies which are bactericidal for meningococcus and/or which recognise conformational epitopes within the meningococcal proteins.
Inventors:
BACCONI MARTA (IT)
BARDELLI MONIA (IT)
BRUNELLI BRUNELLA (IT)
DEWAR VINCENT (BE)
FRIGIMELICA ELISABETTA (IT)
MALZONE CARMINE (IT)
PANSEGRAU WERNER (IT)
VEGGI DANIELE (IT)
BARDELLI MONIA (IT)
BRUNELLI BRUNELLA (IT)
DEWAR VINCENT (BE)
FRIGIMELICA ELISABETTA (IT)
MALZONE CARMINE (IT)
PANSEGRAU WERNER (IT)
VEGGI DANIELE (IT)
Application Number:
PCT/EP2022/079144
Publication Date:
June 01, 2023
Filing Date:
October 19, 2022
Export Citation:
Assignee:
GLAXOSMITHKLINE BIOLOGICALS SA (BE)
International Classes:
G01N33/537; C07K16/12; G01N33/569; G01N33/577; G01N33/58
Domestic Patent References:
| WO2013132040A2 | 2013-09-12 | |||
| WO2009143168A2 | 2009-11-26 | |||
| WO2012031271A1 | 2012-03-08 |
Other References:
BANDIERA SILVIO ET AL: "A single immunogenicity assay for testing potency of combination DTaP vaccines: Simultaneous quantitation of anti-DT, anti-TT, anti-PTxd and anti-FHA antibodies in Guinea-pig serum with a Luminex -xMAP bead-based serological assay", BIOLOGICALS, ACADEMIC PRESS LTD., LONDON, GB, vol. 61, 23 August 2019 (2019-08-23), pages 15 - 21, XP085813642, ISSN: 1045-1056, [retrieved on 20190823], DOI: 10.1016/J.BIOLOGICALS.2019.08.002
SKOTTRUP ET AL: "Rapid determination of Phytophthora infestans sporangia using a surface plasmon resonance immunosensor", JOURNAL OF MICROBIOLOGICAL METHODS, ELSEVIER, AMSTERDAM, NL, vol. 68, no. 3, 16 February 2007 (2007-02-16), pages 507 - 515, XP005892088, ISSN: 0167-7012, DOI: 10.1016/J.MIMET.2006.10.011
YIXIAN WANG ET AL: "Subtractive inhibition assay for the detection of E.coli O157:H7 using surface plasmon resonance", vol. 11, no. 3, 1 January 2011 (2011-01-01), pages 2728 - 2739, XP003029230, ISSN: 1424-8220, Retrieved from the Internet [retrieved on 20110301], DOI: 10.3390/S110302728
MASDOR NOOR AZLINA ET AL: "Subtractive inhibition assay for the detection of Campylobacter jejuni in chicken samples using surface plasmon resonance", SCIENTIFIC REPORTS, vol. 9, no. 1, 1 January 2019 (2019-01-01), pages 13642, XP093022376, Retrieved from the Internet DOI: 10.1038/s41598-019-49672-2
SKOTTRUP ET AL: "Monoclonal antibodies for the detection of Puccinia striiformis urediniospores", MYCOLOGICAL RESEARCH, ELSEVIER, GB, vol. 111, no. 3, 1 March 2007 (2007-03-01), pages 332 - 338, XP022015904, ISSN: 0953-7562, DOI: 10.1016/J.MYCRES.2006.11.010
SKOTTRUP ET AL: "Rapid determination of Phytophthora infestans sporangia using a surface plasmon resonance immunosensor", JOURNAL OF MICROBIOLOGICAL METHODS, ELSEVIER, AMSTERDAM, NL, vol. 68, no. 3, 16 February 2007 (2007-02-16), pages 507 - 515, XP005892088, ISSN: 0167-7012, DOI: 10.1016/J.MIMET.2006.10.011
YIXIAN WANG ET AL: "Subtractive inhibition assay for the detection of E.coli O157:H7 using surface plasmon resonance", vol. 11, no. 3, 1 January 2011 (2011-01-01), pages 2728 - 2739, XP003029230, ISSN: 1424-8220, Retrieved from the Internet
MASDOR NOOR AZLINA ET AL: "Subtractive inhibition assay for the detection of Campylobacter jejuni in chicken samples using surface plasmon resonance", SCIENTIFIC REPORTS, vol. 9, no. 1, 1 January 2019 (2019-01-01), pages 13642, XP093022376, Retrieved from the Internet
SKOTTRUP ET AL: "Monoclonal antibodies for the detection of Puccinia striiformis urediniospores", MYCOLOGICAL RESEARCH, ELSEVIER, GB, vol. 111, no. 3, 1 March 2007 (2007-03-01), pages 332 - 338, XP022015904, ISSN: 0953-7562, DOI: 10.1016/J.MYCRES.2006.11.010
Attorney, Agent or Firm:
BROOM, Ashley (GB)
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