Title:
IN VIVO DELIVERY OF OLIGONUCLEOTIDES
Document Type and Number:
WIPO Patent Application WO/2013/090457
Kind Code:
A3
Abstract:
This invention provides a method for the in vivo delivery of oligonucleotides. The invention utilizes the presence of one or plurality of HES linked to an oligonucleotide to deliver a nucleic acid sequence of interest into the cytoplasm of cells and tissues of live organisms. The delivery vehicle is nontoxic to cells and organisms. Since delivery is sequence-independent and crosses membranes in a receptor-independent manner, the delivered oligonucleotide can target complementary sequences in the cytoplasm as well as in the nucleus of live cells. Sequences of bacterial or viral origin can also be targeted. The method can be used for delivery of genes coding for expression of specific proteins, antisense oligonucleotides, siRNAs, shRNAs, Dicer substrates, miRNAs, anti-miRNAs or any nucleic acid sequence in a living organism. The latter include mammals, plants, and microorganisms such as bacteria, protozoa, and viruses.
Inventors:
PACKARD BEVERLY (US)
KOMORIYA AKIRA (US)
KOMORIYA AKIRA (US)
Application Number:
PCT/US2012/069294
Publication Date:
September 04, 2014
Filing Date:
December 12, 2012
Export Citation:
Assignee:
ONCOIMMUNIN INC (US)
International Classes:
A61K31/7088; A61K9/00; A61K48/00
Foreign References:
| US20090325168A1 | 2009-12-31 | |||
| US20050142581A1 | 2005-06-30 | |||
| US20030224377A1 | 2003-12-04 |
Other References:
PACKARD ET AL.: "A Method in Enzymology for Measuring Hydrolytic Activities in Live Cell Environments", METHODS IN ENZYMOLOGY, vol. 450, 2008, pages 1 - 19, XP008174215
Attorney, Agent or Firm:
SHEA, Jr., Timothy J. et al. (Kessler Goldstein & Fox, P.L.L.C.,1100 New York Avenue, N.W, Washington District of Columbia, US)
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