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Patent Searching and Data


Title:
INCREASING THE STABILITY OF RECOMBINANT ADULT HUMAN APOHEMOGLOBIN
Document Type and Number:
WIPO Patent Application WO2006031840
Kind Code:
A3
Abstract:
The disclosure relates to recombinant adult human apohemoglobin (apo-rHb) in which the stability has been increased by replacement of an amino acid with a counterpart from another organsim, such as a deep sea diving mammal. This mutated apo-rHb may be more stable and/or give higher production yields than unmutated adult human apo-rHb. The mutated apo-rHb may be produced in microorganisms, such as E. coli or yeast cells, or animal erythroid cells. Some apo-rHb of the present disclosure may be used as part of a blood substitute.

Inventors:
OLSON JOHN S (US)
PHILLIPS GEORGE N JR (US)
Application Number:
PCT/US2005/032627
Publication Date:
May 11, 2006
Filing Date:
September 15, 2005
Export Citation:
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Assignee:
UNIV RICE WILLIAM M (US)
WISCONSIN ALUMNI RES FOUND (US)
OLSON JOHN S (US)
PHILLIPS GEORGE N JR (US)
Other References:
FRONTICELLI C. ET AL: "Allosteric modulation by tertiary structure in mammalian hemoglobins", JOURNAL OF BOIOLOGICAL CHEMISTRY, vol. 270, no. 51, 22 December 1995 (1995-12-22), pages 30588 - 30592, XP002996147
DUMOULIN A. ET AL: "The N-terminal sequence affects distant helix interactions in hemoglobin", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 273, no. 52, 25 December 1998 (1998-12-25), pages 35032 - 35038, XP002996148
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