Title:
INDICYANINE DYES AND THE DERIVATIVES THEREOF FOR ANALYSING BIOLOGICAL MICROMOLECULES
Document Type and Number:
WIPO Patent Application WO/2008/127139
Kind Code:
A1
Abstract:
The invention relates to two series of synthesised indolenine pentamethine
dyes (37 compounds) comprising a reactive group in the third and fifth positions
of the indolenine cycle, respectively. A method for producing, extracting and
purifying the dyes and the precursors thereof, consisting in determining the
absorption and fluorescence maxima, molecular extinction factors, fluorescence
quantum yields, relative fluorescence effectiveness at excitation on a wavelength
of 635 nm (detection of 670 nm) and 655 nm (detection of 690 nm), relative light
stability and comparative sensitivity of detection of marked oligonucleotides
on biochips of the claimed dyes, is disclosed. The use of the claimed dyes in the
form of fluorescent marks for oligonucleotide and protein chips is also disclosed.
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Inventors:
CHUDINOV ALEXANDR VASILIEVICH (RU)
KUZNETSOVA VIKTORIA EVGENIEVNA (RU)
ZASEDATELEV ALEXANDR SERGEEVIC (RU)
BARSKY VIKTOR EVGENIEVICH (RU)
KUZNETSOVA VIKTORIA EVGENIEVNA (RU)
ZASEDATELEV ALEXANDR SERGEEVIC (RU)
BARSKY VIKTOR EVGENIEVICH (RU)
Application Number:
PCT/RU2007/000172
Publication Date:
October 23, 2008
Filing Date:
April 11, 2007
Export Citation:
Assignee:
INST MOLEKULYARNOI BIOLOG IM V (RU)
CHUDINOV ALEXANDR VASILIEVICH (RU)
KUZNETSOVA VIKTORIA EVGENIEVNA (RU)
ZASEDATELEV ALEXANDR SERGEEVIC (RU)
BARSKY VIKTOR EVGENIEVICH (RU)
CHUDINOV ALEXANDR VASILIEVICH (RU)
KUZNETSOVA VIKTORIA EVGENIEVNA (RU)
ZASEDATELEV ALEXANDR SERGEEVIC (RU)
BARSKY VIKTOR EVGENIEVICH (RU)
International Classes:
C07D403/06; C07D209/08; C07D209/18; C07D209/24; C09B23/08; G01N33/533
Domestic Patent References:
| WO2002066574A1 | 2002-08-29 | |||
| WO2002026891A1 | 2002-04-04 |
Foreign References:
| EP0342939A1 | 1989-11-23 | |||
| DE19921234A1 | 2000-11-16 | |||
| CN1702118A | 2005-11-30 | |||
| EP1211294A1 | 2002-06-05 |
Other References:
BERTOLINO C.A. ET AL.: "Solvent effect on inducyanine dyes: A computational approach", CHEMICAL PHYSICS, vol. 330, 2006, pages 52 - 59, XP005846068
MADER O. ET AL.: "Structure property analysis of pentamethine indocyanine dyes: Identification of a new dye for life science applications", BIOCONJUGATE CHEM., 2004, pages 70 - 78, XP001222066
MUJUMBAR R.B. ET AL.: "Cyanine dye labeling reagents containing isothiocyanate groups", CYTOMETRY, vol. 10, no. 1, 1989, pages 11 - 19, XP002403542
See also references of EP 2157088A4
MADER O. ET AL., BIOCONJUGATE CHEM, vol. 15, 2004, pages 70 - 78
MANK A.J.G. ET AL., ANAL. CHEM., vol. 67, 1995, pages 1742 - 1748
MUJUMDAR R.B. ET AL., BIOCOJUCG. CHEM, vol. 4, no. 2, 1993, pages 105 - 111
SOUTHWICK ET AL., CYTOMETRY, vol. 11, no. 3, 1990, pages 418 - 30
LIN Y ET AL., BIOCONJUC. CHEM., vol. 13, 2002, pages 605 - 610
LIN Y. ET AL., BIOCONJUC. CHEM., vol. 13, 2002, pages 605 - 610
MANK A.J. G. ET AL., ANAL. CHEM., vol. 67, 1995, pages 1742 - 1748
MADER 0. ET AL.: "Structure Properties of Pentamethine Indocyanine Dyes: Identification of New Dyes for Life Science Applications", BIOCONJUGATE CHEM, vol. 15, 2004, pages 70 - 78, XP001222066, DOI: doi:10.1021/bc034191h
MADER O. ET AL.: "Structure property analysis of pentamethine indocyanine dyes: Identification of a new dye for life science applications", BIOCONJUGATE CHEM., 2004, pages 70 - 78, XP001222066
MUJUMBAR R.B. ET AL.: "Cyanine dye labeling reagents containing isothiocyanate groups", CYTOMETRY, vol. 10, no. 1, 1989, pages 11 - 19, XP002403542
See also references of EP 2157088A4
MADER O. ET AL., BIOCONJUGATE CHEM, vol. 15, 2004, pages 70 - 78
MANK A.J.G. ET AL., ANAL. CHEM., vol. 67, 1995, pages 1742 - 1748
MUJUMDAR R.B. ET AL., BIOCOJUCG. CHEM, vol. 4, no. 2, 1993, pages 105 - 111
SOUTHWICK ET AL., CYTOMETRY, vol. 11, no. 3, 1990, pages 418 - 30
LIN Y ET AL., BIOCONJUC. CHEM., vol. 13, 2002, pages 605 - 610
LIN Y. ET AL., BIOCONJUC. CHEM., vol. 13, 2002, pages 605 - 610
MANK A.J. G. ET AL., ANAL. CHEM., vol. 67, 1995, pages 1742 - 1748
MADER 0. ET AL.: "Structure Properties of Pentamethine Indocyanine Dyes: Identification of New Dyes for Life Science Applications", BIOCONJUGATE CHEM, vol. 15, 2004, pages 70 - 78, XP001222066, DOI: doi:10.1021/bc034191h
Attorney, Agent or Firm:
OBSCHESTVO S OGRANICHENNOI OTVETSTVENNOSTJU "SOJUZPATENT" (5/2Moscow, 5, RU)
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