Title:
INDUCED MALIGNANT STEM CELLS OR PRE-INDUCTION CANCER STEM CELLS CAPABLE OF SELF-REPLICATION OUTSIDE OF AN ORGANISM, PRODUCTION METHOD FOR SAME, AND PRACTICAL APPLICATION FOR SAME
Document Type and Number:
WIPO Patent Application WO/2011/148983
Kind Code:
A1
Abstract:
Disclosed are inducible cancer stem cells which are capable of in vitro self-replication and which are useful in research into cancer treatment and cancer-related drug development. Also disclosed are a production method for the above cells, cancer cells induced from the above stem cells, and a practical application of the cells. The disclosed inducible cancer stem cells which are capable of in vitro self-replication are characterised by satisfying requirements (1) and (2): (1) expressing six genes (POU5F1 gene, NANOG gene, SOX2 gene, ZFP42 gene, LIN28 gene, and TERT gene) selected from among a specified gene group; (2) (a) having an abnormality which is either the modification of an endogenous cancer suppression gene, or the up-regulation of an endogenous cancer related gene.
Inventors:
ISHIKAWA TETSUYA (JP)
Application Number:
PCT/JP2011/062006
Publication Date:
December 01, 2011
Filing Date:
May 25, 2011
Export Citation:
Assignee:
NAT CANCER CT (JP)
ISHIKAWA TETSUYA (JP)
ISHIKAWA TETSUYA (JP)
International Classes:
C12N5/10; A01K67/027; A61K39/00; C12N15/09; C12Q1/02; G01N33/15; G01N33/50
Foreign References:
| JP2008283972A | 2008-11-27 | |||
| JP2008307007A | 2008-12-25 | |||
| JP3229590B2 | 2001-11-19 | |||
| JP3499528B2 | 2004-02-23 |
Other References:
MIYOSHI, N ET AL.: "Defined factors induce reprogramming of gastrointestinal cancer cells.", PROC NATL ACAD SCI U S A., vol. 107, no. 1, 5 January 2010 (2010-01-05), pages 40 - 45, XP055083357
LEE, JS ET AL.: "Brain cancer stem-like cell genesis from p53-deficient mouse astrocytes by oncogenic Ras.", BIOCHEM BIOPHYS RES COMMUN., vol. 365, no. 3, 18 January 2008 (2008-01-18), pages 496 - 502, XP022372609
XU, Q ET AL.: "Isolation of tumour stem-like cells from benign tumours.", BR J CANCER., vol. 101, no. 2, 21 July 2009 (2009-07-21), pages 303 - 311, XP055098334
BAE, KM ET AL.: "Expression of pluripotent stem cell reprogramming factors by prostate tumor initiating cells.", J UROL., vol. 183, no. 5, May 2010 (2010-05-01), pages 2045 - 2053, XP027038755
ILIOPOULOS, D ET AL.: "Inducible formation of breast cancer stem cells and their dynamic equilibrium with non-stem cancer cells via IL6 secretion.", PROC NATL ACAD SCI U S A., vol. 108, no. 4, 25 January 2011 (2011-01-25), pages 1397 - 1402, XP055098845
HOCHEDLINGER K; JAENISCH R ET AL., GENES DEV., vol. 18, 2004, pages 1875 - 1885
NAKAGAWA M; YAMANAKA S ET AL., NAT BIOTECHNOL., vol. 26, 2008, pages 101 - 106
TAKAHASHI K; YAMANAKA S, CELL, vol. 131, 2007, pages 861 - 872
MASAKI H; ISHIKAWA T ET AL., STEM CELL RES., vol. 1, 2008, pages 105 - 115
INTERNATIONAL SOCIETY FOR STEM CELL RESEARCH, 2009, pages 285
UTIKAL J ET AL., J CELL SCI., vol. 122, 2009, pages 3502 - 3510
CARETTE JE ET AL., BLOOD, vol. 115, 2010, pages 4039 - 4042
NAGAI K ET AL., BIOCHEM BIOPHYS RES COMMUN., vol. 395, 2010, pages 258 - 263
VISVADER JE; LINDEMAN GJ, NAT REV CANCER., vol. 8, 2008, pages 755 - 768
TAKAHASHI K; YAMANAKA S ET AL., CELL, vol. 131, 2007, pages 861 - 872
MASAKI H; ISHIKAWA T ET AL., STEM CELL RES., vol. 1, 2008, pages 105 - 115
TOSHIO KITAMURA: "Retrovirus-mediated gene transfer and expression cloning: Powerful tools in functional genomics", EXPERIMENTAL HEMATOLOGY, vol. 31, no. 11, 2003, pages 1007 - 14, XP009118675, DOI: doi:10.1016/j.exphem.2003.07.005
PROC. NATL. ACAD. SCI. USA, vol. 92, 1995, pages 6733 - 6737
NUCLEIC ACIDS RESEARCH, vol. 18, 1990, pages 3587 - 3596
JOURNAL OF VIROLOGY, vol. 72, 1998, pages 8150 - 8157
NUCLEIC ACIDS RES., vol. 23, 1995, pages 3816 - 3821
"Center for iPS Cell Research and Application", vol. 7.4, 2008, INSTITUTE FOR INTEGRATED CELL-MATERIAL SCIENCE, article "Methods of establishing human iPS cells", pages: 1 - 14
THOMSON JA ET AL.: "Embryonic stem cell lines derived from human blastocysts", SCIENCE, vol. 282, no. 5391, 6 November 1998 (1998-11-06), pages 1145 - 7, XP002933311, DOI: doi:10.1126/science.282.5391.1145
ERRATUM IN SCIENCE, vol. 282, no. 5395, 4 December 1998 (1998-12-04), pages 1827
HIROFUMI SUEMORI ET AL.: "Efficient establishment of human embryonic stem cell lines and long term maintenance with stable karyotype by enzymatic bulk passage", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 345, 2006, pages 926 - 32
See also references of EP 2599860A4
LEE, JS ET AL.: "Brain cancer stem-like cell genesis from p53-deficient mouse astrocytes by oncogenic Ras.", BIOCHEM BIOPHYS RES COMMUN., vol. 365, no. 3, 18 January 2008 (2008-01-18), pages 496 - 502, XP022372609
XU, Q ET AL.: "Isolation of tumour stem-like cells from benign tumours.", BR J CANCER., vol. 101, no. 2, 21 July 2009 (2009-07-21), pages 303 - 311, XP055098334
BAE, KM ET AL.: "Expression of pluripotent stem cell reprogramming factors by prostate tumor initiating cells.", J UROL., vol. 183, no. 5, May 2010 (2010-05-01), pages 2045 - 2053, XP027038755
ILIOPOULOS, D ET AL.: "Inducible formation of breast cancer stem cells and their dynamic equilibrium with non-stem cancer cells via IL6 secretion.", PROC NATL ACAD SCI U S A., vol. 108, no. 4, 25 January 2011 (2011-01-25), pages 1397 - 1402, XP055098845
HOCHEDLINGER K; JAENISCH R ET AL., GENES DEV., vol. 18, 2004, pages 1875 - 1885
NAKAGAWA M; YAMANAKA S ET AL., NAT BIOTECHNOL., vol. 26, 2008, pages 101 - 106
TAKAHASHI K; YAMANAKA S, CELL, vol. 131, 2007, pages 861 - 872
MASAKI H; ISHIKAWA T ET AL., STEM CELL RES., vol. 1, 2008, pages 105 - 115
INTERNATIONAL SOCIETY FOR STEM CELL RESEARCH, 2009, pages 285
UTIKAL J ET AL., J CELL SCI., vol. 122, 2009, pages 3502 - 3510
CARETTE JE ET AL., BLOOD, vol. 115, 2010, pages 4039 - 4042
NAGAI K ET AL., BIOCHEM BIOPHYS RES COMMUN., vol. 395, 2010, pages 258 - 263
VISVADER JE; LINDEMAN GJ, NAT REV CANCER., vol. 8, 2008, pages 755 - 768
TAKAHASHI K; YAMANAKA S ET AL., CELL, vol. 131, 2007, pages 861 - 872
MASAKI H; ISHIKAWA T ET AL., STEM CELL RES., vol. 1, 2008, pages 105 - 115
TOSHIO KITAMURA: "Retrovirus-mediated gene transfer and expression cloning: Powerful tools in functional genomics", EXPERIMENTAL HEMATOLOGY, vol. 31, no. 11, 2003, pages 1007 - 14, XP009118675, DOI: doi:10.1016/j.exphem.2003.07.005
PROC. NATL. ACAD. SCI. USA, vol. 92, 1995, pages 6733 - 6737
NUCLEIC ACIDS RESEARCH, vol. 18, 1990, pages 3587 - 3596
JOURNAL OF VIROLOGY, vol. 72, 1998, pages 8150 - 8157
NUCLEIC ACIDS RES., vol. 23, 1995, pages 3816 - 3821
"Center for iPS Cell Research and Application", vol. 7.4, 2008, INSTITUTE FOR INTEGRATED CELL-MATERIAL SCIENCE, article "Methods of establishing human iPS cells", pages: 1 - 14
THOMSON JA ET AL.: "Embryonic stem cell lines derived from human blastocysts", SCIENCE, vol. 282, no. 5391, 6 November 1998 (1998-11-06), pages 1145 - 7, XP002933311, DOI: doi:10.1126/science.282.5391.1145
ERRATUM IN SCIENCE, vol. 282, no. 5395, 4 December 1998 (1998-12-04), pages 1827
HIROFUMI SUEMORI ET AL.: "Efficient establishment of human embryonic stem cell lines and long term maintenance with stable karyotype by enzymatic bulk passage", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 345, 2006, pages 926 - 32
See also references of EP 2599860A4
Attorney, Agent or Firm:
ONO, Shinjiro et al. (JP)
Ono Shinjiro (JP)
Ono Shinjiro (JP)
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