Title:
INDUCED REPLICATION OF ONCOLYTIC ROTAVIRUSES FOR NEOPLASM CONTROL
Document Type and Number:
WIPO Patent Application WO/2019/038578
Kind Code:
A1
Abstract:
The present invention provides a method for producing oncolytic rotaviruses from parental rotaviruses and tumour lines of any origin, with the ability to infect and lyse tumour cells in vitro and in vivo, the cell membranes of which express integrin proteins (αβ3), protein disulfide isomerase (PDI) proteins, heat-shock proteins (Hsp90, Hsp70, Hsp60 or Hsp70). Moreover, the present invention provides a method for treating neoplasms in an animal comprising the administration of oncolytic rotaviruses, selected and adapted to tumour cells, in carrier cells in vitro, and subsequent administration thereof to the animal. The method for administering the oncolytic rotaviruses in carrier cells allows recognition by antibodies to be avoided and release of the rotavirus in tumour cells, thereby infecting same.
Inventors:
GUERRERO FONSECA CARLOS ARTURO (CO)
Application Number:
PCT/IB2017/055142
Publication Date:
February 28, 2019
Filing Date:
August 25, 2017
Export Citation:
Assignee:
UNIV NAT COLOMBIA (CO)
International Classes:
A61K35/765; C12N7/01; C12R1/93
Other References:
MARTIN BAUTISTA, LILIANA: "Analisis de la union del rotavirus a los globulos rojos y el efecto que tiene dicha union en la capacidad infecciosa en líneas tumorales", TESIS DOCTORAL (MAESTRÍA THESIS), 2015, en linea, pages 1 - 87, XP055578365, Retrieved from the Internet [retrieved on 20180115]
GUERRERO CARLOS A ET AL.: "Experimental Adaptation of Rotaviruses to Tumor Cell Lines", PLOS ONE, vol. 11, no. 2, 31 January 2016 (2016-01-31), pages e0147666, XP055578384, ISSN: 1932-6203
MUNGUIA A ET AL.: "Cell carriers to deliver oncolytic viruses to sites of myeloma tumor growth", GENE THERAPY, vol. 15, 1 May 2008 (2008-05-01), GB, XP002764671, ISSN: 0969-7128
DAVIS H E ET AL.: "Charged polymers modulate retrovirus transduction via membrane charge neutralization and virus aggregation", BIOPHYSICAL JOURNAL, vol. 86, no. 2, 31 January 2004 (2004-01-31), pages 1234 - 1242, XP055562198, ISSN: 0006-3495, DOI: doi:10.1016/S0006-3495(04)74197-1
GUERRERO CARLOS A ET AL.: "Experimental Adaptation of Rotaviruses to Tumor Cell Lines", PLOS ONE, vol. 11, no. 2, 31 January 2016 (2016-01-31), pages e0147666, XP055578384, ISSN: 1932-6203
MUNGUIA A ET AL.: "Cell carriers to deliver oncolytic viruses to sites of myeloma tumor growth", GENE THERAPY, vol. 15, 1 May 2008 (2008-05-01), GB, XP002764671, ISSN: 0969-7128
DAVIS H E ET AL.: "Charged polymers modulate retrovirus transduction via membrane charge neutralization and virus aggregation", BIOPHYSICAL JOURNAL, vol. 86, no. 2, 31 January 2004 (2004-01-31), pages 1234 - 1242, XP055562198, ISSN: 0006-3495, DOI: doi:10.1016/S0006-3495(04)74197-1
Attorney, Agent or Firm:
CAMACHO CASTELLANOS, Laura Victoria (CO)
Download PDF: