Title:
INDUCIBLE NUCLEIC ACID TARGETS FOR DETECTION OF PATHOGENS, METHODS AND COMPOSITIONS THEREOF
Document Type and Number:
WIPO Patent Application WO/2012/003412
Kind Code:
A3
Abstract:
The present application describes compositions, methods and kits for rapid detection and identification of various microorganisms using inducible RNA. Methods for rapidly detecting microorganisms by detecting expression of inducible RNA of target genes following induction of a target gene using an inducer are described. Some embodiments describe methods and workflows for rapidly detecting microbes such as, but not limited to, Salmonella spp, Listeria spp. and Vibrio spp. Compositions and kits comprise primer nucleic acid sequences having hybridization specificity for priming amplification of genes of microorganisms (or gene fragments) that are responsive to one or more RNA-inducing agents. In some embodiments, kits and compositions further comprise probe nucleic acid sequences having hybridization specificity for genes responsive to RNA-inducing agents or fragments thereof.
Inventors:
BALACHANDRAN PRIYA (US)
CUMMINGS CRAIG (US)
PETRAUSKENE OLGA (US)
PAWAR HEMANT (US)
TEBBS ROBERT (US)
FURTADO MANOHAR (US)
CUMMINGS CRAIG (US)
PETRAUSKENE OLGA (US)
PAWAR HEMANT (US)
TEBBS ROBERT (US)
FURTADO MANOHAR (US)
Application Number:
PCT/US2011/042723
Publication Date:
February 23, 2012
Filing Date:
June 30, 2011
Export Citation:
Assignee:
LIFE TECHNOLOGIES CORP (US)
BALACHANDRAN PRIYA (US)
CUMMINGS CRAIG (US)
PETRAUSKENE OLGA (US)
PAWAR HEMANT (US)
TEBBS ROBERT (US)
FURTADO MANOHAR (US)
BALACHANDRAN PRIYA (US)
CUMMINGS CRAIG (US)
PETRAUSKENE OLGA (US)
PAWAR HEMANT (US)
TEBBS ROBERT (US)
FURTADO MANOHAR (US)
International Classes:
C12Q1/68
Other References:
DUNAEV T ET AL: "Use of RNA-based genotypic approaches for quantification of viable but non-culturable Salmonella sp. in biosolids.", WATER SCIENCE AND TECHNOLOGY : A JOURNAL OF THE INTERNATIONAL ASSOCIATION ON WATER POLLUTION RESEARCH 2008 LNKD- PUBMED:19029724, vol. 58, no. 9, 2008, pages 1823 - 1828, XP002661220, ISSN: 0273-1223
ARNAUD CHASTANET ET AL: "Comparative genomics reveal novel heat shock regulatory mechanisms in Staphylococcus aureus and other Gram-positive bacteria", MOLECULAR MICROBIOLOGY, vol. 47, no. 4, 1 February 2003 (2003-02-01), pages 1061 - 1073, XP055009509, ISSN: 0950-382X, DOI: 10.1046/j.1365-2958.2003.03355.x
ERIC J. ALM: "Comparative Analysis of Bacterial Gene Expression in Response to Environmental Stress", 2 June 2010 (2010-06-02), XP002661221, Retrieved from the Internet [retrieved on 20111013]
KIM B H ET AL: "Expression of cspH, encoding the cold shock protein in Salmonella enterica serovar Typhimurium UK-1.", JOURNAL OF BACTERIOLOGY OCT 2001 LNKD- PUBMED:11544220, vol. 183, no. 19, October 2001 (2001-10-01), pages 5580 - 5588, XP002661222, ISSN: 0021-9193
BAE HOON KIM ET AL: "Expression of cspH upon nutrient up-shift in Salmonella enterica serovar Typhimurium", ARCHIVES OF MICROBIOLOGY, vol. 182, no. 1, 1 September 2004 (2004-09-01), pages 37 - 43, XP055009512, ISSN: 0302-8933, DOI: 10.1007/s00203-004-0692-2
ARNAUD CHASTANET ET AL: "Comparative genomics reveal novel heat shock regulatory mechanisms in Staphylococcus aureus and other Gram-positive bacteria", MOLECULAR MICROBIOLOGY, vol. 47, no. 4, 1 February 2003 (2003-02-01), pages 1061 - 1073, XP055009509, ISSN: 0950-382X, DOI: 10.1046/j.1365-2958.2003.03355.x
ERIC J. ALM: "Comparative Analysis of Bacterial Gene Expression in Response to Environmental Stress", 2 June 2010 (2010-06-02), XP002661221, Retrieved from the Internet
KIM B H ET AL: "Expression of cspH, encoding the cold shock protein in Salmonella enterica serovar Typhimurium UK-1.", JOURNAL OF BACTERIOLOGY OCT 2001 LNKD- PUBMED:11544220, vol. 183, no. 19, October 2001 (2001-10-01), pages 5580 - 5588, XP002661222, ISSN: 0021-9193
BAE HOON KIM ET AL: "Expression of cspH upon nutrient up-shift in Salmonella enterica serovar Typhimurium", ARCHIVES OF MICROBIOLOGY, vol. 182, no. 1, 1 September 2004 (2004-09-01), pages 37 - 43, XP055009512, ISSN: 0302-8933, DOI: 10.1007/s00203-004-0692-2
Attorney, Agent or Firm:
SUBRAMONY, Priya (P.O. Box 52050Minneapolis, Minnesota, US)
Download PDF: