Title:
INDUCTION OF PLURIPOTENT STEM CELL CELLS FROM GERM CELLS
Document Type and Number:
WIPO Patent Application WO/2013/100140
Kind Code:
A1
Abstract:
The present invention provides a method for effectively inducing pluripotent stem cells from germ stem cells. Pluripotent stem cell cells can be induced with greater efficiency than in conventional methods by using germ stem cells in which Sox2 and/or Oct4 is overexpressed. With regard to the germ stem cells, one or more kind of gene selected from Dnmt1 gene, Dmap1 gene, Dmrt1 gene and Oct1 gene is inhibited, or Sox2 gene and/or Oct4 gene is overexpressed, or alternatively the protein of Sox2 and/or Oct4 is directly introduced. Said method enables pluripotent stem cells to be more efficiently induced from germ stem cells than in conventional methods.
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Inventors:
SHINOHARA TAKASHI (JP)
TAKASHIMA SEIJI (JP)
TAKASHIMA SEIJI (JP)
Application Number:
PCT/JP2012/084138
Publication Date:
July 04, 2013
Filing Date:
December 28, 2012
Export Citation:
Assignee:
UNIV KYOTO (JP)
International Classes:
C12N5/00; C12N15/09
Domestic Patent References:
| WO2005100548A1 | 2005-10-27 |
Foreign References:
| JP2009502124A | 2009-01-29 |
Other References:
HUBSCHER,D. ET AL.: "Generation of Flk1+ cells from Oct4-reprogrammed spermatogonial stem cells", JOURNAL OF STEM CELLS AND REGENERATIVE MEDICINE, vol. 6, no. 2, 2010, pages 47
TAKASHI SHINOHARA: "iPS Saibo Kenkyu no Saizensen iPS Saibo Sakusei-Seigyo nado no Iryo Kiban Gijutsu (CREST Kadai Kenkyu) Analysis of reprogramming process in spermatogonial stem cells", REGENERATIVE MEDICINE, vol. 7, no. 3, 2008, pages 255 - 258
KUIJK,E.W. ET AL.: "PTEN and TRP53 independently suppress Nanog expression in spermatogonial stem cells", STEM CELLS AND DEVELOPMENT, vol. 19, no. 7, 2010, pages 979 - 988, XP055076648
IMAMURA,M. ET AL.: "Transcriptional repression and DNA hypermethylation of a small set of ES cell marker genes in male germline stem cells. art 34", BMC DEVELOPMENTAL BIOLOGY, vol. 6, 2006, pages 16PP, XP021014173
ZHANG,S. ET AL.: "Retinol (vitamin A) maintains self-renewal of pluripotent male germline stem cells (mGSCs) from adult mouse testis", JOURNAL OF CELLULAR BIOCHEMISTRY, vol. 112, no. 4, 2011, pages 1009 - 1021, XP055076649
KHROMOV,T. ET AL.: "Global and gene-specific histone modification profiles of mouse multipotent adult germline stem cells", MOLECULAR HUMAN REPRODUCTION, ADVANCED ACCESS, vol. 17, no. 3, 8 October 2010 (2010-10-08), pages 166 - 174, XP055076650
TAKASHIMA,S. ET AL.: "Abnormal DNA methyltransferase expression in mouse germline stem cells results in spermatogenic defects", BIOLOGY OF REPRODUCTION, vol. 81, no. 1, 2009, pages 155 - 164, XP055076651
SCHOLER,H.R.: "Induction of pluripotency in adult stem cells: iPS, piPS and gPS, inv 73", HUMAN GENE THERAPY, vol. 20, no. 11, 2009, pages 1375 - 13766, XP003031257
YUKO MORIMOTO ET AL.: "In vitro transformation of mouse spermatogonial stem cells", J MAMM OVA RES, vol. 29, no. 2, 1 April 2012 (2012-04-01), pages S4
TAKASHI SHINOHARA: "iPS Saibo Kenkyu no Saizensen iPS Saibo Sakusei-Seigyo nado no Iryo Kiban Gijutsu (CREST Kadai Kenkyu) Analysis of reprogramming process in spermatogonial stem cells", REGENERATIVE MEDICINE, vol. 7, no. 3, 2008, pages 255 - 258
KUIJK,E.W. ET AL.: "PTEN and TRP53 independently suppress Nanog expression in spermatogonial stem cells", STEM CELLS AND DEVELOPMENT, vol. 19, no. 7, 2010, pages 979 - 988, XP055076648
IMAMURA,M. ET AL.: "Transcriptional repression and DNA hypermethylation of a small set of ES cell marker genes in male germline stem cells. art 34", BMC DEVELOPMENTAL BIOLOGY, vol. 6, 2006, pages 16PP, XP021014173
ZHANG,S. ET AL.: "Retinol (vitamin A) maintains self-renewal of pluripotent male germline stem cells (mGSCs) from adult mouse testis", JOURNAL OF CELLULAR BIOCHEMISTRY, vol. 112, no. 4, 2011, pages 1009 - 1021, XP055076649
KHROMOV,T. ET AL.: "Global and gene-specific histone modification profiles of mouse multipotent adult germline stem cells", MOLECULAR HUMAN REPRODUCTION, ADVANCED ACCESS, vol. 17, no. 3, 8 October 2010 (2010-10-08), pages 166 - 174, XP055076650
TAKASHIMA,S. ET AL.: "Abnormal DNA methyltransferase expression in mouse germline stem cells results in spermatogenic defects", BIOLOGY OF REPRODUCTION, vol. 81, no. 1, 2009, pages 155 - 164, XP055076651
SCHOLER,H.R.: "Induction of pluripotency in adult stem cells: iPS, piPS and gPS, inv 73", HUMAN GENE THERAPY, vol. 20, no. 11, 2009, pages 1375 - 13766, XP003031257
YUKO MORIMOTO ET AL.: "In vitro transformation of mouse spermatogonial stem cells", J MAMM OVA RES, vol. 29, no. 2, 1 April 2012 (2012-04-01), pages S4
Attorney, Agent or Firm:
SHOJI, Takashi et al. (JP)
Takashi Shoji (JP)
Takashi Shoji (JP)
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