Title:
INDUSTRIALLY USEFUL MICROORGANISM
Document Type and Number:
WIPO Patent Application WO/2006/057341
Kind Code:
A1
Abstract:
After culturing for a definite period of time, a mutant of wild type Escherichia coli, which has a chromosomal DNA shorter by 470 kbp or more than the chromosomal DNA of the wild type, shows a larger cell count than the wild type. Therefore, a useful substance such as a protein, a peptide, an amino acid, a nucleic acid, a vitamin, a saccharide, an organic acid or a lipid can be efficiency produced by culturing the above-described mutant in a medium and thus allowing it to produce and accumulate the useful substance in the culture medium.
Inventors:
MIZOGUCHI HIROSHI
HARA KIYOTAKA
MORI HIDEO
HARA KIYOTAKA
MORI HIDEO
Application Number:
PCT/JP2005/021691
Publication Date:
June 01, 2006
Filing Date:
November 25, 2005
Export Citation:
Assignee:
KYOWA HAKKO KOGYO KK (JP)
MIZOGUCHI HIROSHI
HARA KIYOTAKA
MORI HIDEO
MIZOGUCHI HIROSHI
HARA KIYOTAKA
MORI HIDEO
International Classes:
C12N15/09; C12N1/20; C12N1/21; C12P7/64; C12P13/00; C12P13/04; C12P17/00; C12P19/00; C12P19/30; C12P19/38; C12P21/02
Foreign References:
| JP2004321169A | 2004-11-18 |
Other References:
MORI H.: "Daichokin no Mini Genome Factory (MGF)", BIOSCIENCE & INDUSTRY, vol. 62, no. 3, 1 March 2004 (2004-03-01), pages 167 - 170, XP003007372
KOLISNYCHENKO V. ET AL: "Engineering a reduced Escherichia coli genome", GENOME RES., vol. 12, no. 4, 2002, pages 640 - 647, XP002317216
FUJIO T.: "Minimum Genome Factory- Process Kakushin ni yoru Kankyo Mondai Kaiketsu heno Torikumi", FINE CHEMICAL, vol. 30, no. 10, 2001, pages 5 - 12, XP003007373
IWAI H. ET AL: "Ikusei Kojo o Mokuteki toshita Daichokin Senshokutai Bubun Kesshitsu Kabu no Screening", THE MOLECULAR BIOLOGY SOCIETY OF JAPAN PROGRAM KOEN YOSHISHU, vol. 26, 2003, pages 856 - 3PA-368, XP003007374
GORYSHIN I.Y. ET AL: "Chromosomal deletion formation system based on Tn5 double transposition:use for making minimal genomes and essential gene analysis", GENOME RES., vol. 13, no. 4, 2003, pages 644 - 653, XP003007375
YU B.J. ET AL: "Minimization of the Escherichia coli genome using a Tn5-targeted Cre/loxP excision system", NAT BIOTECHNOL., vol. 20, no. 10, 2002, pages 1018 - 1023, XP002327128
See also references of EP 1829965A4
KOLISNYCHENKO V. ET AL: "Engineering a reduced Escherichia coli genome", GENOME RES., vol. 12, no. 4, 2002, pages 640 - 647, XP002317216
FUJIO T.: "Minimum Genome Factory- Process Kakushin ni yoru Kankyo Mondai Kaiketsu heno Torikumi", FINE CHEMICAL, vol. 30, no. 10, 2001, pages 5 - 12, XP003007373
IWAI H. ET AL: "Ikusei Kojo o Mokuteki toshita Daichokin Senshokutai Bubun Kesshitsu Kabu no Screening", THE MOLECULAR BIOLOGY SOCIETY OF JAPAN PROGRAM KOEN YOSHISHU, vol. 26, 2003, pages 856 - 3PA-368, XP003007374
GORYSHIN I.Y. ET AL: "Chromosomal deletion formation system based on Tn5 double transposition:use for making minimal genomes and essential gene analysis", GENOME RES., vol. 13, no. 4, 2003, pages 644 - 653, XP003007375
YU B.J. ET AL: "Minimization of the Escherichia coli genome using a Tn5-targeted Cre/loxP excision system", NAT BIOTECHNOL., vol. 20, no. 10, 2002, pages 1018 - 1023, XP002327128
See also references of EP 1829965A4
Download PDF: