Title:
INOPERATIVE-TYPE GLUCORAPHASATIN SYNTHASE GENE AND USE OF SAME
Document Type and Number:
WIPO Patent Application WO/2016/072182
Kind Code:
A1
Abstract:
Provided is a technique with which it is possible to produce a glucoraphasatin-deficient daikon line in a short time at high accuracy, as pertains to a technique for fundamentally overcoming the problems of deterioration of flavor, occurrence of radish odor, and deterioration of quality such as yellowing in processed daikon foods and the like. The present invention pertains to an inoperative-type glucoraphasatin synthase gene having (A) the characteristic of having a mutation associated with complete or partial deletion of the 2-oxoglutarate-iron(II)-dependent oxygenase domain in an encoded protein within an exon constituting a glucoraphasatin synthase gene, and (B) the characteristic of having the glucoraphasatin of the root portion deleted or substantially deleted when the glucoraphasatin synthase gene locus in the daikon genome is a homo-type of this inoperative-type gene.
Inventors:
KAKIZAKI TOMOHIRO (JP)
ISHIDA MASAHIKO (JP)
OHARA TAKAYOSHI (JP)
FUKINO NOBUKO (JP)
ISHIDA MASAHIKO (JP)
OHARA TAKAYOSHI (JP)
FUKINO NOBUKO (JP)
Application Number:
PCT/JP2015/077956
Publication Date:
May 12, 2016
Filing Date:
October 01, 2015
Export Citation:
Assignee:
NAT RES AND DEV AGENCY NAT AGRICULTURE AND FOOD RES ORGANIZATION (JP)
International Classes:
C12N15/09; A01H1/00; A01H5/02; A01H5/04; A01H5/06; A01H5/10; A01H5/12; C12N9/02; C12Q1/68
Domestic Patent References:
| WO2012066949A1 | 2012-05-24 |
Foreign References:
| JP2013198436A | 2013-10-03 |
Other References:
DATABASE DDBJ/EMBL/GenBank [o] 26 July 2012 (2012-07-26), "DEFINITION: TSA: Raphanus sativus RS10991 mRNA sequence", Database accession no. JT795538
TOMOHIRO KAKIZAKI ET AL.: "Daikon ni Okeru Glucoraphasatin Gosei Koso Idenshi no Dotei", BREEDING RESEARCH, 21 March 2015 (2015-03-21), pages 85
TOMOHIRO KAKIZAKI ET AL.: "Daikon ni Okeru Glucoraphasatin Gosei Koso Idenshi no Dotei", BREEDING RESEARCH, 21 March 2015 (2015-03-21), pages 85
Attorney, Agent or Firm:
TANABE YOICHI (JP)
Yoichi Tanabe (JP)
Yoichi Tanabe (JP)
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