Title:
INSULIN-LIKE GROWTH FACTOR (IGF-I) PLASMID-MEDIATED SUPPLEMENTATION FOR THERAPEUTIC APPLICATIONS
Document Type and Number:
WIPO Patent Application WO2004081040
Kind Code:
A3
Abstract:
Composition and method for stimulating angiogenesis, stimulating myogenesis, upregulating angiogenic factors and angiopoietins, and treating the muscular and vascular complications of diabetes. Overall, the embodiments of the invention can be accomplished by delivering a heterologous nucleic acid sequence encoding insulin-like growth factor ("IGF-I") or a functional biological equivalent thereof into the cells of the subject and allowing expression of the encoded gene to occur while the modified cells are within the subject. The nucleic acid sequence may be delivered by a vector system including a synthetic myogenic promoter and a 3' untranslated region. The preferred method to deliver the constitutive or inducible nucleic acid encoding sequences of IGF-I or the functional biological equivalents thereof is directly into the cells of the subject by the process of in vivo electroporation.
More Like This:
Inventors:
RABINOVSKY ERIC D (US)
DRAGHIA-AKLI RUXANDRA (US)
DRAGHIA-AKLI RUXANDRA (US)
Application Number:
PCT/US2004/007295
Publication Date:
March 10, 2005
Filing Date:
March 11, 2004
Export Citation:
Assignee:
ADVISYS INC (US)
BAYLOR COLLEGE MEDICINE (US)
RABINOVSKY ERIC D (US)
DRAGHIA-AKLI RUXANDRA (US)
BAYLOR COLLEGE MEDICINE (US)
RABINOVSKY ERIC D (US)
DRAGHIA-AKLI RUXANDRA (US)
International Classes:
A61K48/00; A61P21/00; C07K14/65; C12N15/85; C12N15/87; A61K38/00; (IPC1-7): C07K14/65; C12N15/85; A61K48/00; A61P21/00
Foreign References:
| US20030018984A1 | 2003-01-23 |
Other References:
COLEMAN M E ET AL: "MYOGENIC VECTOR EXPRESSION OF INSULIN-LIKE GROWTH FACTOR I SIMULATES MUSCLE CELL DIFFERENTIATION AND MYOFIBER HYPERTROPHY IN TRANSGENIC MICE", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 270, no. 20, 19 May 1995 (1995-05-19), pages 12109 - 12116, XP000611917, ISSN: 0021-9258
LI X ET AL: "Synthetic muscle promoters: Activities exceeding naturally occurring regulatory sequences", NATURE BIOTECHNOLOGY 1999 UNITED STATES, vol. 17, no. 3, 1999, pages 241 - 245, XP002209991, ISSN: 1087-0156
DRAGHIA-AKLI RUXANDRA ET AL: "Electrical enhancement of formulated plasmid delivery in animals.", TECHNOLOGY IN CANCER RESEARCH & TREATMENT. UNITED STATES OCT 2002, vol. 1, no. 5, October 2002 (2002-10-01), pages 365 - 372, XP001182428, ISSN: 1533-0346
RABINOVSKY ERIC D ET AL: "Insulin-like growth factor I plasmid therapy promotes in vivo angiogenesis.", MOLECULAR THERAPY: THE JOURNAL OF THE AMERICAN SOCIETY OF GENE THERAPY. UNITED STATES JAN 2004, vol. 9, no. 1, January 2004 (2004-01-01), pages 46 - 55, XP002286448, ISSN: 1525-0016
ALILA H ET AL: "Expression of biologically active human Insulin-like growth factor-I following intramuscular injection of a formulated plasmid in rats", HUMAN GENE THERAPY, XX, XX, vol. 8, no. 15, 10 October 1997 (1997-10-10), pages 1785 - 1795, XP002118452, ISSN: 1043-0342
LI X ET AL: "Synthetic muscle promoters: Activities exceeding naturally occurring regulatory sequences", NATURE BIOTECHNOLOGY 1999 UNITED STATES, vol. 17, no. 3, 1999, pages 241 - 245, XP002209991, ISSN: 1087-0156
DRAGHIA-AKLI RUXANDRA ET AL: "Electrical enhancement of formulated plasmid delivery in animals.", TECHNOLOGY IN CANCER RESEARCH & TREATMENT. UNITED STATES OCT 2002, vol. 1, no. 5, October 2002 (2002-10-01), pages 365 - 372, XP001182428, ISSN: 1533-0346
RABINOVSKY ERIC D ET AL: "Insulin-like growth factor I plasmid therapy promotes in vivo angiogenesis.", MOLECULAR THERAPY: THE JOURNAL OF THE AMERICAN SOCIETY OF GENE THERAPY. UNITED STATES JAN 2004, vol. 9, no. 1, January 2004 (2004-01-01), pages 46 - 55, XP002286448, ISSN: 1525-0016
ALILA H ET AL: "Expression of biologically active human Insulin-like growth factor-I following intramuscular injection of a formulated plasmid in rats", HUMAN GENE THERAPY, XX, XX, vol. 8, no. 15, 10 October 1997 (1997-10-10), pages 1785 - 1795, XP002118452, ISSN: 1043-0342
Download PDF:
Previous Patent: PEPTIDIC ANTIBIOTIC
Next Patent: IDENTIFICATION OF PAIN-RELEVANT GENES OF A SNSR FAMILY (SPECIFIC SENSORY NEURONE RECEPTORS)
Next Patent: IDENTIFICATION OF PAIN-RELEVANT GENES OF A SNSR FAMILY (SPECIFIC SENSORY NEURONE RECEPTORS)