Title:
INTERNAL STANDARDS FOR SPHINGOLIPIDS FOR USE IN MASS SPECTROMETRY
Document Type and Number:
WIPO Patent Application WO2003048784
Kind Code:
A3
Abstract:
The present invention relates to an internal standard for use in mass spectometry to determine the concentration of a test sphingolipid, the internal standard comprising the same oligosaccharyl chain and long chain base sphingosine as the test sphingolipid and having an acyl group of different mass from the test sphingolipid but providing a sphingolipid of the same chemical nature as the test sphingolipid, and methods for the preparation and use thereof.
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Inventors:
WINCHESTER BRYAN (GB)
MILLS KEVIN (GB)
MILLS KEVIN (GB)
Application Number:
PCT/GB2002/005327
Publication Date:
December 18, 2003
Filing Date:
November 27, 2002
Export Citation:
Assignee:
ICH PRODUCTIONS LTD (GB)
WINCHESTER BRYAN (GB)
MILLS KEVIN (GB)
WINCHESTER BRYAN (GB)
MILLS KEVIN (GB)
International Classes:
G01N33/483; G01N33/58; G01N27/62; G01N33/92; (IPC1-7): G01N33/92; G01N33/96
Foreign References:
| US5272138A | 1993-12-21 |
Other References:
SUGIYAMA E ET AL: "A quantitative analysis of serum sulfatide by matrix-assisted laser desorption ionization time-of-flight mass spectrometry with delayed ion extraction.", ANALYTICAL BIOCHEMISTRY, (1999 OCT 1) 274 (1) 90-7., XP001154852
LIEBISCH G ET AL: "Quantitative measurement of different ceramide species from crude cellular extracts by electrospray ionization tandem mass spectrometry (ESI-MS/MS).", JOURNAL OF LIPID RESEARCH, (1999 AUG) 40 (8) 1539-46., XP001154853
GU M ET AL: "Ceramide profiling of complex lipid mixtures by electrospray ionization mass spectrometry.", ANALYTICAL BIOCHEMISTRY, (1997 JAN 15) 244 (2) 347-56., XP001154851
SULLARDS M C: "Analysis of sphingomyelin, glucosylceramide, ceramide, sphingosine, and sphingosine 1-phosphate by tandem mass spectrometry.", METHODS IN ENZYMOLOGY, (2000) 312 32-45., XP001154856
CHEN S ET AL: "Quantitative analysis of the molecular species of monosialogangliosides by continuous-flow fast-atom bombardment mass spectrometry.", RAPID COMMUNICATIONS IN MASS SPECTROMETRY, (1991 DEC) 5 (12) 618-21., XP009018753
MILLS K ET AL: "Synthesis of novel internal standards for the quantitative determination of plasma ceramide trihexoside in Fabry disease by tandem mass spectrometry", FEBS LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 515, no. 1-3, 27 March 2002 (2002-03-27), pages 171 - 176, XP004347763, ISSN: 0014-5793
WHITFIELD P.D. ET AL: "Quantification of galactosylsphingosine in the twitcher mouse using electrospray ionization-tandem mass spectrometry.", JOURNAL OF LIPID RESEARCH, (2001) 42/12 (2092-2095)., December 2001 (2001-12-01), XP002257546
HSU F-F ET AL: "Structural determination of glycosphingolipids as lithiated adducts by electrospray ionization mass spectrometry using low-energy collisional-activated dissociation on a triple stage quadrupole instrument", JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, ELSEVIER SCIENCE INC., NEW YORK, NY, US, vol. 12, no. 1, January 2001 (2001-01-01), pages 61 - 79, XP004227091, ISSN: 1044-0305
LIEBISCH G ET AL: "Quantitative measurement of different ceramide species from crude cellular extracts by electrospray ionization tandem mass spectrometry (ESI-MS/MS).", JOURNAL OF LIPID RESEARCH, (1999 AUG) 40 (8) 1539-46., XP001154853
GU M ET AL: "Ceramide profiling of complex lipid mixtures by electrospray ionization mass spectrometry.", ANALYTICAL BIOCHEMISTRY, (1997 JAN 15) 244 (2) 347-56., XP001154851
SULLARDS M C: "Analysis of sphingomyelin, glucosylceramide, ceramide, sphingosine, and sphingosine 1-phosphate by tandem mass spectrometry.", METHODS IN ENZYMOLOGY, (2000) 312 32-45., XP001154856
CHEN S ET AL: "Quantitative analysis of the molecular species of monosialogangliosides by continuous-flow fast-atom bombardment mass spectrometry.", RAPID COMMUNICATIONS IN MASS SPECTROMETRY, (1991 DEC) 5 (12) 618-21., XP009018753
MILLS K ET AL: "Synthesis of novel internal standards for the quantitative determination of plasma ceramide trihexoside in Fabry disease by tandem mass spectrometry", FEBS LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 515, no. 1-3, 27 March 2002 (2002-03-27), pages 171 - 176, XP004347763, ISSN: 0014-5793
WHITFIELD P.D. ET AL: "Quantification of galactosylsphingosine in the twitcher mouse using electrospray ionization-tandem mass spectrometry.", JOURNAL OF LIPID RESEARCH, (2001) 42/12 (2092-2095)., December 2001 (2001-12-01), XP002257546
HSU F-F ET AL: "Structural determination of glycosphingolipids as lithiated adducts by electrospray ionization mass spectrometry using low-energy collisional-activated dissociation on a triple stage quadrupole instrument", JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, ELSEVIER SCIENCE INC., NEW YORK, NY, US, vol. 12, no. 1, January 2001 (2001-01-01), pages 61 - 79, XP004227091, ISSN: 1044-0305
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