Title:
INTRA-GENOMIC HOMOLOGOUS RECOMBINATION
Document Type and Number:
WIPO Patent Application WO/2021/173480
Kind Code:
A3
Abstract:
Compositions and methods are provided for an inducible, high efficiency intra-genomic homologous recombination (IGHR) system in plants, that can be used for non-chimeric, heritable gene targeting. The advantage of IGHR approach to targeted integration is that every cell contains donor DNA and nuclease-encoding sequences, so that there are many potentially homology-directed targeting events that can occur during plant development.
More Like This:
Inventors:
CHO HYEON-JE (US)
ANAND AJITH (US)
ARLING MAREN L (US)
BARONE PIERLUIGI (US)
GORDON-KAMM WILLIAM JAMES (US)
KUMAR SANDEEP (US)
LENDERTS BRIAN (US)
LOWE KEITH S (US)
SVITASHEV SERGEI (US)
WU XINLI EMILY (US)
ANAND AJITH (US)
ARLING MAREN L (US)
BARONE PIERLUIGI (US)
GORDON-KAMM WILLIAM JAMES (US)
KUMAR SANDEEP (US)
LENDERTS BRIAN (US)
LOWE KEITH S (US)
SVITASHEV SERGEI (US)
WU XINLI EMILY (US)
Application Number:
PCT/US2021/019011
Publication Date:
June 09, 2022
Filing Date:
February 22, 2021
Export Citation:
Assignee:
PIONEER HI BRED INT (US)
International Classes:
C12N5/10; C12N15/09; C12N15/82
Domestic Patent References:
| WO2019217358A1 | 2019-11-14 |
Foreign References:
| US20170121722A1 | 2017-05-04 | |||
| US20180142249A1 | 2018-05-24 |
Other References:
ANAND AJITH; BASS STEVEN H.; WU EMILY; WANG NING; MCBRIDE KEVIN E.; ANNALURU NARAYANA; MILLER MICHAEL; HUA MO; JONES TODD J.: "An improved ternary vector system forAgrobacterium-mediated rapid maize transformation", PLANT MOLECULAR BIOLOGY, SPRINGER, DORDRECHT., NL, vol. 97, no. 1, 23 April 2018 (2018-04-23), NL , pages 187 - 200, XP036500591, ISSN: 0167-4412, DOI: 10.1007/s11103-018-0732-y
KUMAR ET AL.: "Gene Targeting and Transgene Stacking Using Intra Genomic Homologous Recombination in Plants", PLANT METHODS, vol. 12, no. 11, 1 February 2016 (2016-02-01), pages 1 - 7, XP055942571
KEITH LOWE, EMILY WU, NING WANG, GEORGE HOERSTER, CRAIG HASTINGS, MYEONG-JE CHO, CHRIS SCELONGE, BRIAN LENDERTS, MARK CHAMBERLIN, : "Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation", THE PLANT CELL, AMERICAN SOCIETY OF PLANT BIOLOGISTS, US, vol. 28, no. 9, 10 September 2016 (2016-09-10), US , pages 1998 - 2015, XP055318109, ISSN: 1040-4651, DOI: 10.1105/tpc.16.00124
SVITASHEV ET AL.: "Genome Editing in Maize Directed by CRISPR-Cas9 Ribonucleoprotein Complexes", NATURE COMMUNICATIONS, vol. 7, no. 13274, 16 November 2016 (2016-11-16), pages 1 - 7, XP055359029
KUMAR ET AL.: "Gene Targeting and Transgene Stacking Using Intra Genomic Homologous Recombination in Plants", PLANT METHODS, vol. 12, no. 11, 1 February 2016 (2016-02-01), pages 1 - 7, XP055942571
KEITH LOWE, EMILY WU, NING WANG, GEORGE HOERSTER, CRAIG HASTINGS, MYEONG-JE CHO, CHRIS SCELONGE, BRIAN LENDERTS, MARK CHAMBERLIN, : "Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation", THE PLANT CELL, AMERICAN SOCIETY OF PLANT BIOLOGISTS, US, vol. 28, no. 9, 10 September 2016 (2016-09-10), US , pages 1998 - 2015, XP055318109, ISSN: 1040-4651, DOI: 10.1105/tpc.16.00124
SVITASHEV ET AL.: "Genome Editing in Maize Directed by CRISPR-Cas9 Ribonucleoprotein Complexes", NATURE COMMUNICATIONS, vol. 7, no. 13274, 16 November 2016 (2016-11-16), pages 1 - 7, XP055359029
Attorney, Agent or Firm:
VOSNIDOU, Nancy C et al. (US)
Download PDF: