Title:
INTRACELLULAR ANTIGEN BINDING
Document Type and Number:
WIPO Patent Application WO/2016/023898
Kind Code:
A3
Abstract:
The disclosure generally provides a Designed Ankyrin Repeat Protein (DARPin) that specifically binds to an antigen, the DARPin having an N-terminal cap section, at least two Ankyrin Repeat (AR) module sections, and a C-terminal cap section, characterised in that the DARPin has a charge that is less negative than known DARPins. The disclosure also provides for generation of a library of the less negative DARPins.
Inventors:
MINTER RALPH (GB)
JACKSON RON (GB)
KJELLERUP LASSE (DK)
JACKSON RON (GB)
KJELLERUP LASSE (DK)
Application Number:
PCT/EP2015/068451
Publication Date:
April 07, 2016
Filing Date:
August 11, 2015
Export Citation:
Assignee:
MEDIMMUNE LTD (GB)
International Classes:
C07K14/47
Domestic Patent References:
| WO2014191574A1 | 2014-12-04 |
Foreign References:
| US20140005125A1 | 2014-01-02 |
Other References:
DATABASE Geneseq [online] 5 June 2014 (2014-06-05), "Designed ankyrin repeat protein 9.26 (DARPin 9.26), SEQ ID 17.", XP002751320, retrieved from EBI accession no. GSP:BBF25840 Database accession no. BBF25840
DATABASE Geneseq [online] 5 June 2014 (2014-06-05), "Designed ankyrin repeat protein (DARPin), SEQ ID 35.", XP002751321, retrieved from EBI accession no. GSP:BBF25858 Database accession no. BBF25858
BINZ H ET AL: "Designing Repeat Proteins: Well-expressed, Soluble and Stable Proteins from Combinatorial Libraries of Consensus Ankyrin Repeat Proteins", JOURNAL OF MOLECULAR BIOLOGY, ACADEMIC PRESS, UNITED KINGDOM, vol. 332, no. 2, 12 September 2003 (2003-09-12), pages 489 - 503, XP027101280, ISSN: 0022-2836, [retrieved on 20030912], DOI: 10.1016/S0022-2836(03)00896-9
P. MICHAELY ET AL: "A Requirement for Ankyrin Binding to Clathrin during Coated Pit Budding", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 274, no. 50, 10 December 1999 (1999-12-10), US, pages 35908 - 35913, XP055229739, ISSN: 0021-9258, DOI: 10.1074/jbc.274.50.35908
F. PASUTTO ET AL: "Variants in ASB10 are associated with open-angle glaucoma", HUMAN MOLECULAR GENETICS, vol. 21, no. 6, 8 December 2011 (2011-12-08), gb, pages 1336 - 1349, XP055230867, ISSN: 0964-6906, DOI: 10.1093/hmg/ddr572
JAMESJ CRONICAN ET AL: "A Class of Human Proteins that Deliver Functional Proteins into Mammalian Cells InVitro and InVivo", CHEMISTRY AND BIOLOGY, CURRENT BIOLOGY, LONDON, GB, vol. 18, no. 7, 7 July 2011 (2011-07-07), pages 833 - 838, XP028248847, ISSN: 1074-5521, [retrieved on 20110715], DOI: 10.1016/J.CHEMBIOL.2011.07.003
YKELIEN L BOERSMA ET AL: "DARPins and other repeat protein scaffolds: advances in engineering and applications", CURRENT OPINION IN BIOTECHNOLOGY, vol. 22, no. 6, 27 June 2011 (2011-06-27), pages 849 - 857, XP028397473, ISSN: 0958-1669, [retrieved on 20110608], DOI: 10.1016/J.COPBIO.2011.06.004
DATABASE Geneseq [online] 5 June 2014 (2014-06-05), "Designed ankyrin repeat protein (DARPin), SEQ ID 35.", XP002751321, retrieved from EBI accession no. GSP:BBF25858 Database accession no. BBF25858
BINZ H ET AL: "Designing Repeat Proteins: Well-expressed, Soluble and Stable Proteins from Combinatorial Libraries of Consensus Ankyrin Repeat Proteins", JOURNAL OF MOLECULAR BIOLOGY, ACADEMIC PRESS, UNITED KINGDOM, vol. 332, no. 2, 12 September 2003 (2003-09-12), pages 489 - 503, XP027101280, ISSN: 0022-2836, [retrieved on 20030912], DOI: 10.1016/S0022-2836(03)00896-9
P. MICHAELY ET AL: "A Requirement for Ankyrin Binding to Clathrin during Coated Pit Budding", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 274, no. 50, 10 December 1999 (1999-12-10), US, pages 35908 - 35913, XP055229739, ISSN: 0021-9258, DOI: 10.1074/jbc.274.50.35908
F. PASUTTO ET AL: "Variants in ASB10 are associated with open-angle glaucoma", HUMAN MOLECULAR GENETICS, vol. 21, no. 6, 8 December 2011 (2011-12-08), gb, pages 1336 - 1349, XP055230867, ISSN: 0964-6906, DOI: 10.1093/hmg/ddr572
JAMESJ CRONICAN ET AL: "A Class of Human Proteins that Deliver Functional Proteins into Mammalian Cells InVitro and InVivo", CHEMISTRY AND BIOLOGY, CURRENT BIOLOGY, LONDON, GB, vol. 18, no. 7, 7 July 2011 (2011-07-07), pages 833 - 838, XP028248847, ISSN: 1074-5521, [retrieved on 20110715], DOI: 10.1016/J.CHEMBIOL.2011.07.003
YKELIEN L BOERSMA ET AL: "DARPins and other repeat protein scaffolds: advances in engineering and applications", CURRENT OPINION IN BIOTECHNOLOGY, vol. 22, no. 6, 27 June 2011 (2011-06-27), pages 849 - 857, XP028397473, ISSN: 0958-1669, [retrieved on 20110608], DOI: 10.1016/J.COPBIO.2011.06.004
Attorney, Agent or Firm:
WINTER, Christopher (Granta ParkCambridge, Cambridgeshire CB21 6GH, GB)
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