Title:
ION ANALYSIS DEVICE AND ION DISSOCIATION METHOD
Document Type and Number:
WIPO Patent Application WO/2018/190013
Kind Code:
A1
Abstract:
Precursor ions are trapped inside an ion trap (2), after which electrons having a high energy of 30 eV or more are introduced from an electron irradiation unit (7) into the ion trap (2), thereby increasing the valence of the ions by the mutual action between the electrons and the ions. Subsequently, hydrogen radicals are input from a hydrogen radical irradiation unit (5) into the ion trap (2) and the ions are dissociated by the HAD method. In the HAD method, higher valence of the ions results in a higher dissociation efficiency. Thus, even when using an ion source such as, for example, an MALDI ion source in which almost only monovalent ions are generated, it is possible to enhance the dissociation efficiency by increasing the valence of the precursor ions inside the ion trap (2).
Inventors:
TAKAHASHI HIDENORI (JP)
YAMAUCHI SHOSEI (JP)
YAMAUCHI SHOSEI (JP)
Application Number:
PCT/JP2018/007346
Publication Date:
October 18, 2018
Filing Date:
February 27, 2018
Export Citation:
Assignee:
SHIMADZU CORP (JP)
International Classes:
H01J49/42; G01N27/62
Domestic Patent References:
| WO2015133259A1 | 2015-09-11 | |||
| WO2015133259A1 | 2015-09-11 |
Foreign References:
| US20070138386A1 | 2007-06-21 | |||
| JP2007527002A | 2007-09-20 | |||
| JP2001222971A | 2001-08-17 | |||
| US20110049347A1 | 2011-03-03 | |||
| US20160141165A1 | 2016-05-19 | |||
| US20080035841A1 | 2008-02-14 | |||
| US20170221694A1 | 2017-08-03 |
Other References:
TAKAHASHI, HIDENORI ET AL.: "Hydrogen Attachment/Abstraction Dissociation (HAD) of Gas- Phase Peptide Ions for Tandem Mass Spectrometry", ANALYTICAL CHEMISTRY, vol. 88, no. 7, 22 March 2016 (2016-03-22), pages 3810 - 3816, XP055546231
H. TAKAHASHI: "Hydrogen Attachment/Abstraction Dissociation (HAD) of Gas-Phase Peptide Ions for Tandem Mass Spectrometry", ANALYTICAL CHEMISTRY, vol. 88, no. 7, 2016, pages 3810 - 3816, XP055546231, doi:10.1021/acs.analchem.5b04888
R. A. ZUBAREV: "Electron capture dissociation for structural characterization of multiply charged protein cations", ANALYTICAL CHEMISTRY, vol. 72, no. 3, 2000, pages 563 - 573, XP055525797, doi:10.1021/ac99081
B. A. BUDNIK: "MH 2+ ion production from protonated polypeptides by electron impact: observation and determination of ionization energies and a cross-section", CHEMICAL PHYSICS LETTERS, vol. 316, no. 1, 2000, pages 19 - 23
M. EVA FUNG: "Electron ionization dissociation of singly and multiply charged peptides", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 131, no. 29, 2009, pages 9977 - 9985, XP055110970, doi:10.1021/ja8087407
DONALD RAPP: "Cross Sections for Dissociative Ionization of Molecules by Electron Impact", JOURNAL OF CHEMICAL PHYSICS, vol. 42, 1965, pages 4081
K. BREUKER: "Detailed unfolding and folding of gaseous ubiquitin ions characterized by electron capture dissociation", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 124, no. 22, 2002, pages 6407 - 6420
CS KONCZ: "Calculated cross sections for the single ionization of fullerenes by electron impact", NUCLEAR INSTRUMENTS AND METHODS IN PHYSICS RESEARCH SECTION B, vol. 124, no. 2, 1997, pages 435 - 437, XP004073286, doi:10.1016/S0168-583X(96)00808-7
LUCIANO H. DI STEFANO: "Size-Dependent Hydrogen Atom Attachment to Gas-Phase Hydrogen-Deficient Polypeptide Radical Cations", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 140, no. 2, 2018, pages 531 - 533
See also references of EP 3611755A4
H. TAKAHASHI: "Hydrogen Attachment/Abstraction Dissociation (HAD) of Gas-Phase Peptide Ions for Tandem Mass Spectrometry", ANALYTICAL CHEMISTRY, vol. 88, no. 7, 2016, pages 3810 - 3816, XP055546231, doi:10.1021/acs.analchem.5b04888
R. A. ZUBAREV: "Electron capture dissociation for structural characterization of multiply charged protein cations", ANALYTICAL CHEMISTRY, vol. 72, no. 3, 2000, pages 563 - 573, XP055525797, doi:10.1021/ac99081
B. A. BUDNIK: "MH 2+ ion production from protonated polypeptides by electron impact: observation and determination of ionization energies and a cross-section", CHEMICAL PHYSICS LETTERS, vol. 316, no. 1, 2000, pages 19 - 23
M. EVA FUNG: "Electron ionization dissociation of singly and multiply charged peptides", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 131, no. 29, 2009, pages 9977 - 9985, XP055110970, doi:10.1021/ja8087407
DONALD RAPP: "Cross Sections for Dissociative Ionization of Molecules by Electron Impact", JOURNAL OF CHEMICAL PHYSICS, vol. 42, 1965, pages 4081
K. BREUKER: "Detailed unfolding and folding of gaseous ubiquitin ions characterized by electron capture dissociation", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 124, no. 22, 2002, pages 6407 - 6420
CS KONCZ: "Calculated cross sections for the single ionization of fullerenes by electron impact", NUCLEAR INSTRUMENTS AND METHODS IN PHYSICS RESEARCH SECTION B, vol. 124, no. 2, 1997, pages 435 - 437, XP004073286, doi:10.1016/S0168-583X(96)00808-7
LUCIANO H. DI STEFANO: "Size-Dependent Hydrogen Atom Attachment to Gas-Phase Hydrogen-Deficient Polypeptide Radical Cations", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 140, no. 2, 2018, pages 531 - 533
See also references of EP 3611755A4
Attorney, Agent or Firm:
KYOTO INTERNATIONAL PATENT LAW OFFICE (JP)
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