Title:
ISOLATION AND ANALYSIS OF THIOL PROTEIN MATTER USING GOLD NANO-PARTICLES
Document Type and Number:
WIPO Patent Application WO/2011/038375
Kind Code:
A3
Abstract:
A method of rapidly and accurately identifying and analyzing thiol proteins in a sample using gold nanoparticles. Disclosed are embodiments of a flow device for isolation, fractionation and subsequent instrumental analysis of thiol containing proteins from various samples or tissue sources using gold nanoparticles. Also disclosed are embodiments of a flow device for detecting, isolating and fractionating S-nitrosated proteins and peptides for subsequent analysis, including identification of S-nitrosation sites.
Inventors:
ZHANG XUEJI (US)
MUTUS BULENT (CA)
MUTUS BULENT (CA)
Application Number:
PCT/US2010/050462
Publication Date:
May 26, 2011
Filing Date:
September 28, 2010
Export Citation:
Assignee:
WORLD PREC INSTR INC
ZHANG XUEJI (US)
MUTUS BULENT (CA)
ZHANG XUEJI (US)
MUTUS BULENT (CA)
International Classes:
C07F1/00
Foreign References:
| US20090206245A1 | 2009-08-20 | |||
| US20070281362A1 | 2007-12-06 |
Other References:
CEDERVALL ET AL.: "Understanding the nanoparticle-protein corona using methods to quantify exchange rates and affinities of proteins for nanoparticles.", PROC NATL ACAD SCI USA, vol. 104, no. 7, 2007, pages 2050 - 2055
JIA ET AL.: "Potential oxidative stress of gold nanoparticles by induced-NO releasing in serum.", J AM CHEM SOC., vol. 131, no. 1, 14 January 2009 (2009-01-14), pages 40 - 41
DERAKHSHAN ET AL.: "Unbiased identification of cysteine S-nitrosylation sites on proteins.", NAT PROTOC., vol. 2, no. 7, 2007, pages 1685 - 1691
HASAN ET AL.: "The Fate of Sulfur-Bound Hydrogen on Formation of Self-Assembled Thiol Monolayers on Gold: 1 H NMR Spectroscopic Evidence from Solutions of Gold Clusters.", J AM CHEM SOC., vol. 24, no. 7, 2002, pages 1132 - 1133
COLES ET AL.: "S-Nitrosoglutathione inactivation of the mitochondrial and cytosolic BCAT proteins: S-nitrosation and S-thiolation.", BIOCHEMISTRY, vol. 48, no. 3, 27 January 2009 (2009-01-27), pages 645 - 656
DATABASE GENBANK [online] 3 May 1996 (1996-05-03), "Albumin [Homo sapiens].", retrieved from http://www.ncbi.nlm.nih.gov/protein/AAA98797.1j Database accession no. AAA98797.
JIA ET AL.: "Potential oxidative stress of gold nanoparticles by induced-NO releasing in serum.", J AM CHEM SOC., vol. 131, no. 1, 14 January 2009 (2009-01-14), pages 40 - 41
DERAKHSHAN ET AL.: "Unbiased identification of cysteine S-nitrosylation sites on proteins.", NAT PROTOC., vol. 2, no. 7, 2007, pages 1685 - 1691
HASAN ET AL.: "The Fate of Sulfur-Bound Hydrogen on Formation of Self-Assembled Thiol Monolayers on Gold: 1 H NMR Spectroscopic Evidence from Solutions of Gold Clusters.", J AM CHEM SOC., vol. 24, no. 7, 2002, pages 1132 - 1133
COLES ET AL.: "S-Nitrosoglutathione inactivation of the mitochondrial and cytosolic BCAT proteins: S-nitrosation and S-thiolation.", BIOCHEMISTRY, vol. 48, no. 3, 27 January 2009 (2009-01-27), pages 645 - 656
DATABASE GENBANK [online] 3 May 1996 (1996-05-03), "Albumin [Homo sapiens].", retrieved from http://www.ncbi.nlm.nih.gov/protein/AAA98797.1j Database accession no. AAA98797.
Attorney, Agent or Firm:
YALE, Guy D. et al. (Yale & Ristas LLP,750 Main Street - Suite 140, Hartford CT, US)
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