Title:
ISOLATION OF INOSITOL FROM PLANT MATERIALS
Document Type and Number:
WIPO Patent Application WO2004050887
Kind Code:
A3
Abstract:
Phytate and/or phytin and/or phytic acid in an aqueous slurry of plant mateiral is partially hydrolyzed by incubating the slurry with an enzyme product enriched in phytase. The soluble fraction of the slurry is separated into anionic and neutral fractions. The anionic fraction is then hydrolyzed further, and the hydrolyzate is separated into second ionic and neutral fractions. The second neutral fraction thus obtained is rich in inositol, and does not contain significant quantities of other sugars which would be hard to separate from it.
Inventors:
NEWKIRK REX W (CA)
MAENZ DAVID D (CA)
CLASSEN HENRY L (CA)
MAENZ DAVID D (CA)
CLASSEN HENRY L (CA)
Application Number:
PCT/CA2003/001849
Publication Date:
September 23, 2004
Filing Date:
November 28, 2003
Export Citation:
Assignee:
MCN BIOPRODUCTS INC (CA)
NEWKIRK REX W (CA)
MAENZ DAVID D (CA)
CLASSEN HENRY L (CA)
NEWKIRK REX W (CA)
MAENZ DAVID D (CA)
CLASSEN HENRY L (CA)
International Classes:
C12P7/02; (IPC1-7): C12P7/02
Foreign References:
| US6156563A | 2000-12-05 |
Other References:
PATENT ABSTRACTS OF JAPAN vol. 0172, no. 36 (C - 1057) 13 May 1993 (1993-05-13)
KAUFMAN H. W. AND KLEINBERG I.: "Hydrolysis of Phytate and its Inositol Phosphate Intermediates by an Acid and an Alkaline Phosphatase.", ARCHS ORAL BIOL., vol. 20, 1975, pages 157 - 160, XP000902746
LIM P E ET AL: "THE PHYTASES II. PROPERTIES OF PHYTASE REACTIONS F1 AND F2 FROM WHEAT BRAN AND THE MYO-INOSITOL PHOSPHATES PRODUCED BY FRACTION F2", BIOCHIMICA ET BIOPHYSICA ACTA, AMSTERDAM, NL, vol. 302, no. 2 E43, 1973, pages 316 - 328, XP000972485, ISSN: 0006-3002
MULLANEY E J ET AL: "The term phytase comprises several different classes of enzymes", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 312, no. 1, 5 December 2003 (2003-12-05), pages 179 - 184, XP004473248, ISSN: 0006-291X
DATABASE CHEMABS [online] CHEMICAL ABSTRACTS SERVICE, COLUMBUS, OHIO, US; 1998, PIZZOFERRATO, L. ET AL PIZZOFERRATO, L. ET AL: "31P NMR spectra of myo- inositol phosphates in model systems and foods 31P NMR spectra of myo- inositol phosphates in model systems and foods", XP000227293, accession no. STN Database accession no. 1998:145617
CHEN Q-C ET AL: "Separation of phytic acid and other related inositol phosphates by high-performance ion chromatography and its applications", JOURNAL OF CHROMATOGRAPHY A, ELSEVIER SCIENCE, NL, vol. 1018, no. 1, 7 November 2003 (2003-11-07), pages 41 - 52, XP004463107, ISSN: 0021-9673
KAUFMAN H. W. AND KLEINBERG I.: "Hydrolysis of Phytate and its Inositol Phosphate Intermediates by an Acid and an Alkaline Phosphatase.", ARCHS ORAL BIOL., vol. 20, 1975, pages 157 - 160, XP000902746
LIM P E ET AL: "THE PHYTASES II. PROPERTIES OF PHYTASE REACTIONS F1 AND F2 FROM WHEAT BRAN AND THE MYO-INOSITOL PHOSPHATES PRODUCED BY FRACTION F2", BIOCHIMICA ET BIOPHYSICA ACTA, AMSTERDAM, NL, vol. 302, no. 2 E43, 1973, pages 316 - 328, XP000972485, ISSN: 0006-3002
MULLANEY E J ET AL: "The term phytase comprises several different classes of enzymes", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 312, no. 1, 5 December 2003 (2003-12-05), pages 179 - 184, XP004473248, ISSN: 0006-291X
DATABASE CHEMABS [online] CHEMICAL ABSTRACTS SERVICE, COLUMBUS, OHIO, US; 1998, PIZZOFERRATO, L. ET AL PIZZOFERRATO, L. ET AL: "31P NMR spectra of myo- inositol phosphates in model systems and foods 31P NMR spectra of myo- inositol phosphates in model systems and foods", XP000227293, accession no. STN Database accession no. 1998:145617
CHEN Q-C ET AL: "Separation of phytic acid and other related inositol phosphates by high-performance ion chromatography and its applications", JOURNAL OF CHROMATOGRAPHY A, ELSEVIER SCIENCE, NL, vol. 1018, no. 1, 7 November 2003 (2003-11-07), pages 41 - 52, XP004463107, ISSN: 0021-9673
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