Title:
ISOLATION METHOD FOR INNER CELL MASSES AND METHOD FOR PREPARING EMBRYONIC STEM CELL LINES USING THE SAME
Document Type and Number:
WIPO Patent Application WO/2010/114193
Kind Code:
A1
Abstract:
The present invention relates to an isolation method for inner cell masses and a method for preparing embryonic stem cell lines using the same. More particularly, the present invention relates to an isolation method for inner cell masses by placing a blastocyst, from which a zona pellucida has been removed, on feeder cells and covering the blastocyst with a micro cover glass; and pressurizing the blastocyst by the weight of the micro cover glass for a predetermined amount of time to the isolated inner cell masses. Thus, it is possible to harvest inner cell masses with a significantly higher yield than before. Accordingly, the isolation method can establish and proliferate embryonic stem cell lines effectively.
Inventors:
KIM CHANG-HYUN (KR)
Application Number:
PCT/KR2009/002806
Publication Date:
October 07, 2010
Filing Date:
May 27, 2009
Export Citation:
Assignee:
KIM CHANG-HYUN (KR)
International Classes:
C12N5/0735; C12Q1/24
Foreign References:
| US7294508B2 | 2007-11-13 | |||
| US20070010010A1 | 2007-01-11 | |||
| KR100715366B1 | 2007-05-07 | |||
| KR100567535B1 | 2006-04-03 |
Other References:
SUSANNE STROM ET AL.: "Mechanical isolation of the inner cell mass is effective in derivation of new human embryonic stem cell lines.", HUMAN REPRODUCTION., vol. 22, no. 12, December 2007 (2007-12-01), pages 3051 - 3058, XP002585157, DOI: doi:10.1093/HUMREP/DEM335
CORTES J. L. ET AL.: "Whole-blastocyst culture followed by laser drilling technology enhances the efficiency of inner cell mass isolation and embryos: new insights for derivation of human embryonic stem cell lines.", STEM CELLS AND DEVELOPMENT., vol. 17, no. 2, April 2008 (2008-04-01), pages 255 - 267
CORTES J. L. ET AL.: "Whole-blastocyst culture followed by laser drilling technology enhances the efficiency of inner cell mass isolation and embryos: new insights for derivation of human embryonic stem cell lines.", STEM CELLS AND DEVELOPMENT., vol. 17, no. 2, April 2008 (2008-04-01), pages 255 - 267
Attorney, Agent or Firm:
DARAE PATENT FIRM (KR)
특허법인 다래 (KR)
특허법인 다래 (KR)
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