Title:
ISOLATION OF SOLUBLE PROTEINS FROM AGGREGATED CASEIN-CONTAINING MIXTURES
Document Type and Number:
WIPO Patent Application WO/2016/055064
Kind Code:
A3
Abstract:
The present invention relates to a method for separating at least one soluble protein fraction from an aggregated casein-containing material, the method comprises the steps of: (i) providing the aggregated casein-containing material; (ii) Contacting the aggregated casein-containing material with a chromatographic support allowing one or more soluble protein(s) present in the aggregated casein-containing material to be retained by the chromatographic support; (iii) Obtaining a permeate fraction from the chromatographic support comprising aggregated casein; (iv) Optionally washing the chromatographic support; (v) Subjecting the chromatographic support to at least one elution buffer obtaining at least one soluble protein fraction from the chromatographic support; and wherein the chromatographic support comprises one or more mixed-mode ligands capable of binding the soluble proteins from the aggregated casein-containing material.
More Like This:
Inventors:
LIHME ALLAN OTTO FOG (DK)
HANSEN MARIE BENDIX (DK)
HANSEN MARIE BENDIX (DK)
Application Number:
PCT/DK2015/000039
Publication Date:
June 09, 2016
Filing Date:
October 06, 2015
Export Citation:
Assignee:
UPFRONT CHROMATOGRAPHY AS (DK)
International Classes:
A23C9/146; A23J1/20; B01D15/38; C07K1/16; C07K16/04
Domestic Patent References:
| WO1999015024A1 | 1999-04-01 | |||
| WO2010037736A1 | 2010-04-08 | |||
| WO1995019714A1 | 1995-07-27 |
Foreign References:
| US5756680A | 1998-05-26 | |||
| US5986063A | 1999-11-16 |
Other References:
GAGNON PETE: "IgG aggregate removal by charged-hydrophobic mixed mode chromatography", CURRENT PHARMACEUTICAL BIOTECHNOLOGY, NETHERLANDS, vol. 10, no. 4, 1 June 2009 (2009-06-01), pages 434 - 439, XP009161599, ISSN: 1873-4316
YE X ET AL: "ISOLATION OF LACTOPEROXIDASE, LACTOFERRIN, ALPHA-LACTALBUMIN, BETA-LACTOGLOBULIN B AND BETA-LACTOGLOBULIN A FROM BOVINE RENNET WHEY USING ION EXCHANGE CHROMATOGRAPHY", INTERNATIONAL JOURNAL OF BIOCHEMISTRY AND CELL BIOLOGY, PERGAMON, GB, vol. 32, no. 11, 1 November 2000 (2000-11-01), pages 1143 - 1150, XP001064940, ISSN: 1357-2725, DOI: 10.1016/S1357-2725(00)00063-7
GERBERDING S J ET AL: "Preparative ion-exchange chromatography of proteins from dairy whey", JOURNAL OF CHROMATOGRAPHY, ELSEVIER SCIENCE PUBLISHERS B.V, NL, vol. 808, no. 1-2, 29 May 1998 (1998-05-29), pages 141 - 151, XP004122668, ISSN: 0021-9673, DOI: 10.1016/S0021-9673(98)00103-4
YE X ET AL: "ISOLATION OF LACTOPEROXIDASE, LACTOFERRIN, ALPHA-LACTALBUMIN, BETA-LACTOGLOBULIN B AND BETA-LACTOGLOBULIN A FROM BOVINE RENNET WHEY USING ION EXCHANGE CHROMATOGRAPHY", INTERNATIONAL JOURNAL OF BIOCHEMISTRY AND CELL BIOLOGY, PERGAMON, GB, vol. 32, no. 11, 1 November 2000 (2000-11-01), pages 1143 - 1150, XP001064940, ISSN: 1357-2725, DOI: 10.1016/S1357-2725(00)00063-7
GERBERDING S J ET AL: "Preparative ion-exchange chromatography of proteins from dairy whey", JOURNAL OF CHROMATOGRAPHY, ELSEVIER SCIENCE PUBLISHERS B.V, NL, vol. 808, no. 1-2, 29 May 1998 (1998-05-29), pages 141 - 151, XP004122668, ISSN: 0021-9673, DOI: 10.1016/S0021-9673(98)00103-4
Attorney, Agent or Firm:
LARSEN & BIRKEHOLM A/S (Banegårdpladsen 1, 1570 Copenhagen V, DK)
Download PDF:
Previous Patent: ADDITIONAL OUTSIDE MIRROR SYSTEM FOR A TOWING VEHICLE-TRAILER COMBINATION
Next Patent: MAIN SHAFT FIXTURE
Next Patent: MAIN SHAFT FIXTURE