Title:
ISOTHERMAL AMPLIFICATION BASED ASSAY FOR THE DETECTION AND QUANTITATION OF ALPHA-FETOPROTEIN mRNA
Document Type and Number:
WIPO Patent Application WO2004038366
Kind Code:
A3
Abstract:
The present invention is directed to isothermal transcription based assays for the detection and quantification of alpha-fetoprotein (AFP) mRNA. The present invention is also directed to oligonucleotides for amplifying AFP mRNA and probes for use in the detection and quantification of the amplification product. The present invention is also directed to detecting hepatocellular carcinoma, metastasis thereof and tumor recurrence by analyzing peripheral blood for the presence of AFP mRNA.
Inventors:
SHURTLIFF ROXANNE
LEE EUN MI
LEE EUN MI
Application Number:
PCT/US2003/031766
Publication Date:
December 29, 2005
Filing Date:
October 07, 2003
Export Citation:
Assignee:
BIO MERIEUX INC (US)
International Classes:
C12Q1/68; (IPC1-7): C07H21/04; C12P19/34; C12Q1/68
Foreign References:
| DE19509169A1 | 1996-09-19 |
Other References:
WONG I. ET AL.: "Hematogenous Dissemination of Hepatocytes and Tumor Cells after Surgical Resection of Hepatocellular Carcinoma:A Quantitative Analysis.", CLINICAL CANCER RESEARCH, vol. 5, no. 12, 1 December 1999 (1999-12-01), pages 4021 - 4027, XP002993001
WONG I. ET AL.: "Circulating Tumor Cell mRNAs in Peripheral Blood From Hepatocellular Carcinoma Patients under radiotherapy, Surgical Resection or Chemotherapy: A Quantitative Evaluation.", CANCER LETTERS, vol. 167, June 2001 (2001-06-01), pages 183 - 191, XP002987761
WONG I. ET AL.: "Quantative Comparison of Alpha-Fetoprotein and Albumin mRNA Levels in Hepatocellular Carcinoma/Adenoma, Non-TumorLiver and Blood: Implication in Cancer Detection and Monitoring.", CANCER LETTERS., vol. 156, no. 2, 11 August 2000 (2000-08-11), pages 141 - 149, XP002993002
See also references of EP 1585968A4
WONG I. ET AL.: "Circulating Tumor Cell mRNAs in Peripheral Blood From Hepatocellular Carcinoma Patients under radiotherapy, Surgical Resection or Chemotherapy: A Quantitative Evaluation.", CANCER LETTERS, vol. 167, June 2001 (2001-06-01), pages 183 - 191, XP002987761
WONG I. ET AL.: "Quantative Comparison of Alpha-Fetoprotein and Albumin mRNA Levels in Hepatocellular Carcinoma/Adenoma, Non-TumorLiver and Blood: Implication in Cancer Detection and Monitoring.", CANCER LETTERS., vol. 156, no. 2, 11 August 2000 (2000-08-11), pages 141 - 149, XP002993002
See also references of EP 1585968A4
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