Title:
ISOTOPE DISTRIBUTION DATA PRODUCTION METHOD
Document Type and Number:
WIPO Patent Application WO/2023/002548
Kind Code:
A1
Abstract:
An isotope distribution data production method according to the present invention includes: preparing, as samples each containing a metabolite of cells cultured in a culture medium containing a substrate labeled with a stable isotope, a plurality of analysis samples in which the concentrations of the metabolite are varied; subjecting the plurality of analysis sample to mass spectrometry separately under the same analysis conditions; and subjecting the plurality of analysis samples to the analysis of mass spectrum data obtained by the mass spectrometry to identify the type of the metabolite contained in each of the analysis samples and to determine the number of metabolites included in a metabolite group consisting of a metabolite of a non-labeled type and/or a metabolite of a labeled type and the signal intensity of a mass peak corresponding to each of all isotopomers included in the metabolite group. Subsequently, the number of the metabolites included in the metabolite group and the signal intensities of a mass peak of each of the metabolites included in the metabolite group and corresponding to the all types of the metabolites, which have been determined above, are compared among the plurality of analysis samples to select an analysis sample to be used for the determination of an isotope distribution of each metabolite. The data about the isotope distributions of the metabolites, which are obtained by the analysis of the mass spectrum data for the selected analysis sample for each metabolite are integrated together to produce an isotope distribution data of the metabolites.
Inventors:
OKAMOTO MAMI (JP)
YAMADA YOHEI (JP)
OKAHASHI NOBUYUKI (JP)
MATSUDA FUMIO (JP)
YAMADA YOHEI (JP)
OKAHASHI NOBUYUKI (JP)
MATSUDA FUMIO (JP)
Application Number:
PCT/JP2021/027052
Publication Date:
January 26, 2023
Filing Date:
July 19, 2021
Export Citation:
Assignee:
SHIMADZU CORP (JP)
UNIV OSAKA (JP)
UNIV OSAKA (JP)
International Classes:
C12Q1/02; G01N27/62
Foreign References:
| JP2005245440A | 2005-09-15 | |||
| JP2005034149A | 2005-02-10 | |||
| JP2010529457A | 2010-08-26 | |||
| JP2018040802A | 2018-03-15 |
Attorney, Agent or Firm:
KYOTO INTERNATIONAL PATENT LAW OFFICE (JP)
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