Title:
JOINT DETECTION METHODS FOR LYMPHOMA FUSION GENES AND DIAGNOSTIC KITS THEREOF
Document Type and Number:
WIPO Patent Application WO/2011/157222
Kind Code:
A1
Abstract:
The invention discloses joint detection methods for lymphoma fusion genes and diagnostic kits thereof. After primers and probes for the mRNAs of lymphoma fusion genes API2-MALT1 (A1446-M814), API2-MALT1 (A1446-M1123), API2-MALT1 (A1446-M1150), NPM-ALK are designed, the probes are covalently bonded to the beads to form probe-bead mixtures, which are hybridized with the RT-PCR amplification products. After streptavidin-phycoerythrin is added, fluorescent signals from different beads can be detected, and it can be determined whether or not the sample to be detected contains the lymphoma fusion genes, and the expression profiles of the fusion genes can be determined.
Inventors:
SHAO TANG (CN)
YUAN FUMEI (CN)
CHEN QING (CN)
YUAN FUMEI (CN)
CHEN QING (CN)
Application Number:
PCT/CN2011/075849
Publication Date:
December 22, 2011
Filing Date:
June 17, 2011
Export Citation:
Assignee:
MEDI GENETECH CO LTD (CN)
SHAO TANG (CN)
YUAN FUMEI (CN)
CHEN QING (CN)
SHAO TANG (CN)
YUAN FUMEI (CN)
CHEN QING (CN)
International Classes:
C12Q1/68; G01N21/64; G01N33/574
Foreign References:
| CN101955995A | 2011-01-26 | |||
| GB2463401A | 2010-03-17 |
Other References:
YANG WEN-XIU: "Expression of the Variants of API2-MALT 1 Fusion Gene and Its Significance in Primary MALT Lymphoma.", CHINESE JOURNAL OF CLINICAL ONCOLOGY., vol. 31, no. 10, 2004, pages 550 - 553
ZHAO YAN-XIA, EFFECTS OF RNA INTERFERENCE ON NPM-ALK FUSION GENE EXPRESSION IN ANAPLASTIC LARGE-CELL LYMPHOMA CELLS., vol. 7, no. 3, June 2005 (2005-06-01), pages 202 - 206
ZHAO YAN-XIA, EFFECTS OF RNA INTERFERENCE ON NPM-ALK FUSION GENE EXPRESSION IN ANAPLASTIC LARGE-CELL LYMPHOMA CELLS., vol. 7, no. 3, June 2005 (2005-06-01), pages 202 - 206
Attorney, Agent or Firm:
DING & ASSOCIATES PATENT TRADEMARK & LAW (CN)
上海浦一知识产权代理有限公司 (CN)
上海浦一知识产权代理有限公司 (CN)
Download PDF:
Claims: