Title:
KETOL-ACID REDUCTOISOMERASES WITH IMPROVED PERFORMANCE PROPERTIES
Document Type and Number:
WIPO Patent Application WO/2013/048599
Kind Code:
A3
Abstract:
The present invention relates to recombinant microorganisms comprising at least one nucleic acid molecule encoding a ketol-acid reductoisomerase (KARI) or modified NADH-dependent variant thereof, wherein said KARI is at least about 60% identical to SEQ ID NO: 2. In various aspects of the invention, the recombinant microorganisms may comprise an isobutanol producing metabolic pathway and can be used in methods of making isobutanol.
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Inventors:
BUELTER THOMAS (US)
LIES DOUGLAS (US)
PORTER-SCHEINMAN STEPHANIE (US)
SMITH CHRISTOPHER (US)
MEINHOLD PETER (US)
BERRY RUTH (US)
SNOW CHRISTOPHER (US)
LIES DOUGLAS (US)
PORTER-SCHEINMAN STEPHANIE (US)
SMITH CHRISTOPHER (US)
MEINHOLD PETER (US)
BERRY RUTH (US)
SNOW CHRISTOPHER (US)
Application Number:
PCT/US2012/042624
Publication Date:
August 29, 2013
Filing Date:
June 15, 2012
Export Citation:
Assignee:
GEVO INC (US)
CALIFORNIA INST OF TECHN (US)
BUELTER THOMAS (US)
LIES DOUGLAS (US)
PORTER-SCHEINMAN STEPHANIE (US)
SMITH CHRISTOPHER (US)
MEINHOLD PETER (US)
BERRY RUTH (US)
SNOW CHRISTOPHER (US)
BASTIAN SABINE (US)
CALIFORNIA INST OF TECHN (US)
BUELTER THOMAS (US)
LIES DOUGLAS (US)
PORTER-SCHEINMAN STEPHANIE (US)
SMITH CHRISTOPHER (US)
MEINHOLD PETER (US)
BERRY RUTH (US)
SNOW CHRISTOPHER (US)
BASTIAN SABINE (US)
International Classes:
C12N1/20; C12N9/90; C12P7/16
Domestic Patent References:
| WO2009085953A2 | 2009-07-09 |
Foreign References:
| US7910342B2 | 2011-03-22 | |||
| US20110177571A1 | 2011-07-21 |
Other References:
DATABASE ENA SLACKIA EXIGUA ATCC 700122 KETOL-ACID REDUCTOISOMERASE.: "EBI Direct Submission EEZ61613.", accession no. EZ61613
DATABASE GENBANK 4 June 2010 (2010-06-04), "Cryptobacterium curtum DSM 15641, complete genome.", retrieved from http:/lwww.ncbi.nlm.nih.govlnuccore/CP001682 accession no. P001682
DATABASE GENBANK 4 June 2010 (2010-06-04), "Eggerthella lenta DSM 2243, complete genome.", retrieved from http://www.nchi.nlm.nih.govlnuccore/257473675 accession no. P001726.1
BASTIAN ET AL.: "Engineered ketol-acid reductoisomerase and alcohol dehydrogenase enable anaerobic 2-methylpropan-1-ol production at theoretical yield in Escherichia coli.", METAB ENG., vol. 13, no. 3, May 2011 (2011-05-01), pages 345 - 52
DATABASE UNIPROT 31 May 2011 (2011-05-31), "Ketol-acid reductoisomerase.", retrieved from http://www.uniprot.org/uniprotlDOWGKO.txt?version=9 accession no. OWGKO_9ACTN.
DATABASE GENBANK 21 May 2010 (2010-05-21), "Gordonibacter pamelaeae 7-10-1-b draft genome.", retrieved from http://www.ncbi.nlm.nih.gov/nuccore/FP929047 accession no. P929047
TYAGI ET AL.: "The crystal structure of a bacterial class II ketol-acid reductoisomerase: domain conservation and evolution.", PROTEIN SCI., vol. 14, no. 12, 2005, pages 3089 - 3100
ATSUMI ET AL.: "Engineering the isobutanol biosynthetic pathway in Escherichia coli by comparison of three aldehyde reductase/alcohol dehydrogenase genes.", APPL MICROBIOL BIOTECHNOL., vol. 85, no. 3, 2010, pages 651 - 657, XP019778484
DATABASE GENBANK 4 June 2010 (2010-06-04), "Cryptobacterium curtum DSM 15641, complete genome.", retrieved from http:/lwww.ncbi.nlm.nih.govlnuccore/CP001682 accession no. P001682
DATABASE GENBANK 4 June 2010 (2010-06-04), "Eggerthella lenta DSM 2243, complete genome.", retrieved from http://www.nchi.nlm.nih.govlnuccore/257473675 accession no. P001726.1
BASTIAN ET AL.: "Engineered ketol-acid reductoisomerase and alcohol dehydrogenase enable anaerobic 2-methylpropan-1-ol production at theoretical yield in Escherichia coli.", METAB ENG., vol. 13, no. 3, May 2011 (2011-05-01), pages 345 - 52
DATABASE UNIPROT 31 May 2011 (2011-05-31), "Ketol-acid reductoisomerase.", retrieved from http://www.uniprot.org/uniprotlDOWGKO.txt?version=9 accession no. OWGKO_9ACTN.
DATABASE GENBANK 21 May 2010 (2010-05-21), "Gordonibacter pamelaeae 7-10-1-b draft genome.", retrieved from http://www.ncbi.nlm.nih.gov/nuccore/FP929047 accession no. P929047
TYAGI ET AL.: "The crystal structure of a bacterial class II ketol-acid reductoisomerase: domain conservation and evolution.", PROTEIN SCI., vol. 14, no. 12, 2005, pages 3089 - 3100
ATSUMI ET AL.: "Engineering the isobutanol biosynthetic pathway in Escherichia coli by comparison of three aldehyde reductase/alcohol dehydrogenase genes.", APPL MICROBIOL BIOTECHNOL., vol. 85, no. 3, 2010, pages 651 - 657, XP019778484
Attorney, Agent or Firm:
BLINKA, Thomas A. et al. (777 6th Street N.W.,Suite 110, Washington District of Columbia, US)
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