KETOREDUCTASE MUTANT AND APPLICATION THEREOF

WIPO Patent Application WO/2019/140682

A1

Provided are a ketoreductase mutant and an application thereof. The amino acid sequence of the ketoreductase mutant is an amino acid sequence obtained by mutating the amino acid sequence shown in SEQ ID NO: 1, and the mutation comprises at least one of the following mutation sites: position 6, position 94, position 96, position 117, position 144, position 156, position 193, position 205, position 224, position 176, position 85 and position 108; alternatively, the amino acid sequence of the ketoreductase mutant has a mutation site in a mutated amino acid sequence and an amino acid sequence having 80% or more homology with the mutated amino acid sequence. The ketoreductase mutants have high stability, especially the resistance to acetone and isopropanol such that when the mutants are used in the preparation of a chiral hydroxyl heterocyclic substance, the components of the reaction system are simplified, costs are lowered, and the prepared chiral alcohol has an ee value of as high as 99.8%.

HONG HAO (CN)
JAMES GAGE (CN)
LU JIANGPING (CN)
XU XINGFU (CN)
YU WENYAN (CN)
ZHANG NA (CN)
MA YULEI (CN)
CHENG YIBING (CN)
MU HUIYAN (CN)

PCT/CN2018/073614

July 25, 2019

January 22, 2018

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JILIN ASYMCHEM LABORATORIES CO LTD (CN)

C12N9/04; C12N1/21; C12N15/53; C12N15/70; C12P17/00; C12P17/04; C12R1/19

WO2011005527A2

2011-01-13

CN105624125A

2016-06-01

DATABASE PROTEIN 6 August 2013 (2013-08-06), ANONYMOUS: "Oxidoreductase [Acetobacter pasteurianus]", XP055625503, retrieved from NCBI Database accession no. WP_020944327
See also references of EP 3744836A4

KANGXIN PARTNERS, P.C. (CN)

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