Title:
KIT FOR DETECTING MCR GENE, DETECTION METHOD AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2020/133638
Kind Code:
A1
Abstract:
Disclosed are a kit for detecting an MCR gene, a detection method and the use thereof. The kit comprises PCR group A primers and PCR group B primers, wherein the PCR group A primers comprise: a primer pair shown as SEQ ID NO: 1 and SEQ ID NO: 2, a primer pair shown as SEQ ID NO: 3 and SEQ ID NO: 4, a primer pair shown as SEQ ID NO: 5 and SEQ ID NO: 6, a primer pair shown as SEQ ID NO: 7 and SEQ ID NO: 8 and a primer pair shown as SEQ ID NO: 9 and SEQ ID NO: 10; the PCR group B primers comprise: a primer pair shown as SEQ ID NO: 11 and SEQ ID NO: 12, and a primer pair shown as SEQ ID NO: 13 and SEQ ID NO: 14.
Inventors:
HU SHUANGFANG (CN)
KE YUEBIN (CN)
LIU WEIWEN (CN)
LYU ZIQUAN (CN)
SHEN JIANZHONG (CN)
KE YUEBIN (CN)
LIU WEIWEN (CN)
LYU ZIQUAN (CN)
SHEN JIANZHONG (CN)
Application Number:
PCT/CN2019/074897
Publication Date:
July 02, 2020
Filing Date:
February 13, 2019
Export Citation:
Assignee:
SHENZHEN CENTER FOR DISEASE CONTROL AND PREVENTION SHENZHEN HEALTH INSPECTION CENTER SHENZHEN INST O (CN)
International Classes:
C12Q1/68
Foreign References:
| CN108384851A | 2018-08-10 |
Other References:
LESCAT, M. ET AL.: "Rapid Multiplex Polymerase Chain Reaction for Detection of Mcr-1 to Mcr-5 Genes", DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, vol. 92, no. 4, 22 April 2018 (2018-04-22), pages 267 - 269, XP085530470
REBELO, A.R. ET AL.: "Multiplex PCR for Detection of Plasmid-Mediated Colistin Resistance Determinants, Mcr-1, Mcr-2, Mcr-3, Mcr-4 And Mcr-5 for Surveillance Purposes", EURO SURVEILL., vol. 23, no. 6, 8 February 2018 (2018-02-08), pages 1 - 11, XP055721155
REBELO, A.R. ET AL.: "Multiplex PCR for Detection of Plasmid-Mediated Colistin Resistance Determinants, Mcr-1, Mcr-2, Mcr-3, Mcr-4 And Mcr-5 for Surveillance Purposes", EURO SURVEILL., vol. 23, no. 6, 8 February 2018 (2018-02-08), pages 1 - 11, XP055721155
Attorney, Agent or Firm:
YOGO PATENT & TRADE MARK AGENCY LIMITED COMPANY (CN)
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