Title:
KIT FOR DETECTING AND TYPING DENGUE VIRUSES BY REVERSE TRANSCRIPTION PCR AND DETECTION METHOD THEREOF
Document Type and Number:
WIPO Patent Application WO/2016/078553
Kind Code:
A1
Abstract:
Disclosed are a kit for detecting and typing dengue viruses by reverse transcription PCR and a detection method thereof. The kit for detecting and typing the dengue viruses by the reverse transcription PCR comprises primers, probes and an FRET-PCR standard substance, and is characterized in that the primers are an upstream primer and a downstream primer; the probes are a 6-FAM probe and a Cy5 probe; the upstream primer has a nucleotide sequence of SEQ ID No.1; the 6-FAM probe has a nucleotide sequence of SEQ ID No.2; the Cy5 probe has a nucleotide sequence of SEQ ID No.3; and the downstream primer has a nucleotide sequence of SEQ ID No.4.
Inventors:
WANG CHENGMING (CN)
ZHANG JILEI (CN)
LU GUANGWU (CN)
ZHANG JILEI (CN)
LU GUANGWU (CN)
Application Number:
PCT/CN2015/094635
Publication Date:
May 26, 2016
Filing Date:
November 16, 2015
Export Citation:
Assignee:
UNIV YANGZHOU (CN)
International Classes:
C12Q1/70; C12Q1/68; C12R1/93
Foreign References:
CN104328222A | 2015-02-04 | |||
CN102268487A | 2011-12-07 | |||
CN102154519A | 2011-08-17 |
Attorney, Agent or Firm:
BEIJING JINGDA LAW FIRM (CN)
北京市京大律师事务所 (CN)
北京市京大律师事务所 (CN)
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