Title:
KIT FOR DETECTION OF PATHOGEN RNA AND ANIMAL OR HUMAN GENE RNA, AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/266946
Kind Code:
A1
Abstract:
A kit for the detection of pathogen RNA and animal or human gene RNA, the kit comprising a primer pair for the detection of pathogen RNA and animal or human gene RNA by RT-nested RPA, a primer pair for the detection of internal reference genes by RT-nested RPA, and a primer pair for the detection of pathogen RNA and animal or human gene RNA for simultaneously performing detection reactions in the same reaction tube. The present kit for the detection of pathogen RNA and animal or human gene RNA implements the detection of RNA levels of pathogen target genes and internal reference genes in the same tube and increases the quality control of internal reference genes on the experimental process, avoiding false negative results. The present kit for the detection of novel coronavirus SARS-CoV-2 can at minimum detect 1 copy/uL of SARS-CoV-2 viral RNA fragments and has ultra-high sensitivity, providing new technical support for the early discovery, early isolation and early treatment of novel coronavirus SARS-CoV-2, and having very good prospects for application.
Inventors:
HUANG WANQIU (CN)
HUANG JIAN (CN)
HUANG JIAN (CN)
Application Number:
PCT/CN2021/102134
Publication Date:
December 29, 2022
Filing Date:
June 24, 2021
Export Citation:
Assignee:
HUANG WANQIU (CN)
HUANG JIAN (CN)
HUANG JIAN (CN)
International Classes:
C12Q1/70
Foreign References:
| CN112739833A | 2021-04-30 | |||
| CN112553373A | 2021-03-26 | |||
| CN111926117A | 2020-11-13 | |||
| CN111500776A | 2020-08-07 | |||
| CN112266986A | 2021-01-26 | |||
| CN112481358A | 2021-03-12 | |||
| CN112458210A | 2021-03-09 | |||
| US20030219792A1 | 2003-11-27 |
Attorney, Agent or Firm:
SHULUN & PARTNERS (SHANGHAI) (CN)
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