KIT FOR IMMUNOASSAY OF HUMAN TISSUE PLASMINOGEN ACTIVATOR/HUMAN PLASMINOGEN ACTIVATOR INHIBITOR COMPLEX AND METHOD OF IMMUNOASSAY

Title:

KIT FOR IMMUNOASSAY OF HUMAN TISSUE PLASMINOGEN ACTIVATOR/HUMAN PLASMINOGEN ACTIVATOR INHIBITOR COMPLEX AND METHOD OF IMMUNOASSAY

Document Type and Number:

WIPO Patent Application WO/1991/005257

Kind Code:

A1

Abstract:

A kit for the immunoassay of a human tissue plasminogen activator/human plasminogen activator inhibitor complex in a human specimen, which comprises: (i) a monoclonal antibody (a first antibody) against a human plasminogen activator inhibitor bound to an insoluble solid carrier having a mirror-finished surface, (ii) a polyclonal antibody (a second antibody) against an enzyme-labelled human tissue plasminogen activator, (iii) a substrate for determining enzymatic activity and a reaction stopper, (iv) a diluent, and (v) a detergent containing a nonionic surfactant with a HLB value of at least 16; a method of immunoassay of the complex in a human specimen with the kit; and a method of immunoassay of an active human plasminogen activator inhibitor.

Inventors:

HINO SCHUICHIRO (JP)
ITOU KAZUHIKO (JP)
HASEGAWA RYOICHI (JP)
OKAMOTO NAOMI (JP)
NORO ATSUSHI (JP)
MURAKAMI TOSHINOBU (JP)
SAKATA YOICHI (JP)

Application Number:

PCT/JP1990/001269

Publication Date:

April 18, 1991

Filing Date:

October 02, 1990

Export Citation:

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Assignee:

TEIJIN LTD (JP)

International Classes:

G01N33/543; G01N33/86; (IPC1-7): G01N33/53; G01N33/573

Foreign References:

JPS59163565A	1984-09-14
JPS58148963A	1983-09-05
JPS6271861A	1987-04-02
JPS6036964A	1985-02-26
JPS5997057A	1984-06-04
JPS5968674A	1984-04-18
JPS5332114A	1978-03-27
JPS63500564A	1988-03-03
JPS59174759A	1984-10-03

Other References: