Title:
KIT AND METHOD FOR DETECTION OF MICRORNA
Document Type and Number:
WIPO Patent Application WO/2016/129941
Kind Code:
A1
Abstract:
The present invention relates to a kit and a method for detection of an miRNA and, more specifically, to an miRNA detection kit and an miRNA detection method using the kit, the kit comprising: a primer for reverse transcription, which consists of a first hybridization oligonucleotide comprising a nucleic acid sequence which hybridizes specifically to the 3'-end of an miRNA or a poly A tailed miRNA to which poly A is linked via a poly A polymerase, and a first adaptor oligonucleotide comprising any nucleic acid sequence which does not hybridize to a target miRNA; a primer for extension, which consists of a second hybridization oligonucleotide capable of hybridizing specifically to a cDNA reverse transcribed from the miRNA or the poly A tailed miRNA excluding a portion corresponding to the 3'-end of the miRNA or the poly A tailed miRNA hybridized to the first hybridization oligonucleotide, and a second adaptor oligonucleotide comprising any nucleic acid sequence which does not hybridize to the cDNA; and a forward primer selected within the second adaptor oligonucleotide.
Inventors:
LEE JAE HUN (KR)
Application Number:
PCT/KR2016/001411
Publication Date:
August 18, 2016
Filing Date:
February 11, 2016
Export Citation:
Assignee:
HEIMBIOTEK INC (KR)
International Classes:
C12Q1/68; C12N15/113
Foreign References:
| US8383344B2 | 2013-02-26 | |||
| US20140295434A1 | 2014-10-02 | |||
| EP2198059B1 | 2014-12-03 | |||
| US20130045885A1 | 2013-02-21 | |||
| KR20090017670A | 2009-02-18 |
Attorney, Agent or Firm:
HAN, YUN HO (KR)
한윤호 (KR)
한윤호 (KR)
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