Title:
L-ASPARTIC ACID α-DECARBOXYLASE MUTANT AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2020/062961
Kind Code:
A1
Abstract:
An L-aspartic acid α-decarboxylase mutant and an application thereof; the mutant is obtained by mutating lysine at position 221 of L-aspartic acid α-decarboxylase into arginine and by mutating glycine at position 369 into alanine; the obtained new mutant enzyme has better temperature tolerance. K221R and G369A recombinant strains undergo high-density fermentation, sodium L-aspartate is used as a substrate, and β-alanine is prepared by means of a whole-cell catalytic reaction.
Inventors:
ZHOU ZHEMIN (CN)
LIU ZHONGMEI (CN)
ZHOU LI (CN)
CUI WENJING (CN)
YE WENQI (CN)
GUO JUNLING (CN)
WANG CHAO (CN)
LIU ZHONGMEI (CN)
ZHOU LI (CN)
CUI WENJING (CN)
YE WENQI (CN)
GUO JUNLING (CN)
WANG CHAO (CN)
Application Number:
PCT/CN2019/092292
Publication Date:
April 02, 2020
Filing Date:
June 21, 2019
Export Citation:
Assignee:
UNIV JIANGNAN (CN)
International Classes:
C12N9/88; C12N1/21; C12N15/60; C12N15/70; C12P13/06; C12R1/19
Foreign References:
| CN109055346A | 2018-12-21 | |||
| CN109402097A | 2019-03-01 | |||
| CN107988194A | 2018-05-04 | |||
| CN107828714A | 2018-03-23 |
Other References:
YASUYUKI ARAKANE: "Molecular and Functional Analyses of Amino Acid Deca- rboxylases Involved in Cuticle Tanning in Tribolium castaneum", THE JOURNAL OF BIOLOGICAL CHEMISTRY, 12 June 2009 (2009-06-12), ISSN: 0021-9258
QIN MO: "Identification of mutations restricting autocatalytic activa- tion of bacterial L-aspartate alpha-decarboxylase", AMINO ACIDS, vol. 50, no. 10, 2 August 2018 (2018-08-02), pages 1433 - 1450, XP036589416, ISSN: 1438-2199
WANLI PE I: "Molecular engineering of L-aspartate-a-decarboxylase for improved activity and catalytic stability", APPL MICROBIOL BIOTECHNOL, vol. 101, no. 15, 6 June 2017 (2017-06-06), pages 6015 - 6021, XP036281421, ISSN: 1432-0614
QIN MO: "Identification of mutations restricting autocatalytic activa- tion of bacterial L-aspartate alpha-decarboxylase", AMINO ACIDS, vol. 50, no. 10, 2 August 2018 (2018-08-02), pages 1433 - 1450, XP036589416, ISSN: 1438-2199
WANLI PE I: "Molecular engineering of L-aspartate-a-decarboxylase for improved activity and catalytic stability", APPL MICROBIOL BIOTECHNOL, vol. 101, no. 15, 6 June 2017 (2017-06-06), pages 6015 - 6021, XP036281421, ISSN: 1432-0614
Attorney, Agent or Firm:
HARBIN SHINEIP INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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