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Title:
L-GLUTAMATE DEHYDROGENASE MUTANT AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2020/233451
Kind Code:
A1
Abstract:
Disclosed in the present invention is an L-glutamate dehydrogenase mutant, the sequence of the L-glutamate dehydrogenase mutant being a sequence in which amino acid residue A at position 175 in SEQ ID NO. 1 is mutated to be G, and amino acid residue V at position 386 is mutated to be an amino acid residue having less steric hindrance. Further disclosed in the present invention is an application of the described L-amino acid dehydrogenase mutant in the preparation of L-glufosinate-ammonium or a salt thereof. When the L-glutamate dehydrogenase mutant of the present invention is used to prepare L-glufosinate-ammonium or a salt thereof, compared to an L-glutamate dehydrogenase mutant in which only position 175 or 386 is mutated, the specific enzyme activity is higher. Therefore, the action efficiency of the enzyme is improved, reaction costs are reduced, and industrial production is facilitated.

Inventors:
TIAN ZHENHUA (CN)
CHENG ZHANBING (CN)
DING SHAONAN (CN)
JIAO QI (CN)
XU WENXUAN (CN)
HUANG YAO (CN)
JIANG FENG (CN)
Application Number:
PCT/CN2020/089775
Publication Date:
November 26, 2020
Filing Date:
May 12, 2020
Export Citation:
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Assignee:
ABIOCHEM BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12N9/06; C12N5/10; C12N15/53; C12N15/63; C12P13/04
Foreign References:
CN108588045A2018-09-28
CN108588045A2018-09-28
CN107502647A2017-12-22
CN106978453A2017-07-25
CN201910434350A2019-05-23
CN1053669C2000-06-21
US6936444B12005-08-30
US4389488A1983-06-21
EP0382113A11990-08-16
US5221737A1993-06-22
EP0344683A21989-12-06
CN1284858C2006-11-15
CN106978453A2017-07-25
CN108588045A2018-09-28
CN201810162629A2018-02-27
US8017797B22011-09-13
US9834802B22017-12-05
Other References:
"Genbank", Database accession no. WP_012293812.1
J. SAMBROOK: "Molecular Cloning: A Laboratory Manual"
See also references of EP 3974522A4
Attorney, Agent or Firm:
SHANGHAI BESHINING LAW OFFICE (CN)
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