Title:
LACTASE, LACTASE PREPARATION, GENE, RECOMBINANT VECTOR, AND TRANSFORMANT
Document Type and Number:
WIPO Patent Application WO/2023/038057
Kind Code:
A1
Abstract:
Provided is a lactase preparation that has excellent lactose degradation action in milk. The lactase of one aspect of the present invention has lactase activity described by one or a combination of (1)–(6). (1) The amount of lactase protein added to milk to make the remaining lactose concentration no more than 0.1% after 16 hours at 10°C is no more than 15.1 mg/L. (2) The amount of lactase protein added to milk to make the remaining lactose concentration no more than 0.01% after 16 hours at 10°C is no more than 30.2 mg/L. (3) The amount of lactase protein added to milk to make the remaining lactose concentration no more than 0.1% after 24 hours at 10°C is no more than 10.1 mg/L. (4) The amount of lactase protein added to milk to make the remaining lactose concentration no more than 0.01% after 24 hours at 10°C is no more than 15.1 mg/L. (5) When the lactase is added to milk in an amount that makes the final concentration 1.4 LYU/ml, the remaining lactose concentration after 24 hours at 10°C is no more than 0.1%. (6) When the lactase is added to milk in an amount that makes the final concentration 2.1 LYU/ml, the remaining lactose concentration after 24 hours at 10°C is no more than 0.01%.
Inventors:
ODAKA REI (JP)
SANO RYOKO (JP)
IWASAKI TAKUMI (JP)
BABA MASAHIRO (JP)
SABURI WATARU (JP)
MORI HARUHIDE (JP)
SANO RYOKO (JP)
IWASAKI TAKUMI (JP)
BABA MASAHIRO (JP)
SABURI WATARU (JP)
MORI HARUHIDE (JP)
Application Number:
PCT/JP2022/033563
Publication Date:
March 16, 2023
Filing Date:
September 07, 2022
Export Citation:
Assignee:
GODO SHUSEI KK (JP)
International Classes:
C12N15/56; A23C7/04; A23C9/12; A23C21/02; A23C23/00; A23L33/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/38; C12N15/63
Domestic Patent References:
WO2019194062A1 | 2019-10-10 |
Foreign References:
JP2016208998A | 2016-12-15 | |||
JP2004534527A | 2004-11-18 |
Other References:
KEISUKE MATSUMOTO ET.AL.: "Characterization and utilization of β-galactosidase from Lactobacillus and Bifidobacterium", RAKUNO KAGAKU, SHOKUHIN NO KENKYU - JAPANESE JOURNAL OF DAIRY AND FOOD SCIENCE, NIHON RAKUNO KAGAKKAI, SENDAI, JP, vol. 39, no. 6, 1 January 1990 (1990-01-01), JP , pages A - A-248, XP009544334, ISSN: 0385-0218
KARLINALTSCHUL, PRO. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 5873 - 5877
J. MOL. BIOL., vol. 215, 1990, pages 403 - 410
J. SAMBROOK: "Molecular Cloning: A Laboratory Manual", 1989
KARLINALTSCHUL, PRO. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 5873 - 5877
J. MOL. BIOL., vol. 215, 1990, pages 403 - 410
J. SAMBROOK: "Molecular Cloning: A Laboratory Manual", 1989
Attorney, Agent or Firm:
ITOH, Atsushi et al. (JP)
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