Title:
β-LACTOGLOBULIN-VITAMIN CONJUGATE COMPRISING SUGAR ALCOHOLS AND PREPARATION METHOD THEREOF
Document Type and Number:
WIPO Patent Application WO/2020/134772
Kind Code:
A1
Abstract:
Disclosed are a β-lactoglobulin-vitamin conjugate comprising sugar alcohols and a preparation method thereof. The β-lactoglobulin-vitamin conjugate comprising sugar alcohols comprises β-lactoglobulin that has been subjected to glycosylation treatment with high voltage pulses, VE, sorbitol and maltitol. The glycosylation treatment with high voltage pulses involves treating a 1 mg/mL β-lactoglobulin solution prepared with a 0.01 mol/L, Ph 7.4 phosphate buffer solution in a high voltage electric field for 80 μs, the field intensity of the high voltage electric field being 30 kV/cm, and the electric field waveform being a unipolar square wave. Modification of β-lactoglobulin by the glycosylation treatment with high voltage pulses alters the secondary and tertiary structures of β-lactoglobulin, thus effectively reducing protein sensitization. Further, addition of the sugar alcohols as a natural emulsifier enhances the rate of conjugation of the β-lactoglobulin-vitamin conjugate.
Inventors:
ZUO QILE (CN)
HAN FEIFEI (CN)
MAO BAOXING (CN)
LI MIAN (US)
ZHU XUAN (CN)
CHEN JIE (CN)
CHEN YUEWEN (CN)
SHI LIHUA (CN)
ZHANG WENYAO (CN)
HAN FEIFEI (CN)
MAO BAOXING (CN)
LI MIAN (US)
ZHU XUAN (CN)
CHEN JIE (CN)
CHEN YUEWEN (CN)
SHI LIHUA (CN)
ZHANG WENYAO (CN)
Application Number:
PCT/CN2019/120410
Publication Date:
July 02, 2020
Filing Date:
November 22, 2019
Export Citation:
Assignee:
ZHEJIANG HUAKANG PHARMACEUTICAL CO LTD (CN)
International Classes:
C07K14/47; A23C19/09; C07K1/107
Foreign References:
| CN103783169A | 2014-05-14 | |||
| CN109535242A | 2019-03-29 |
Other References:
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TIAN, MING: "Effect of Pulsed Electric Fields Treatment on Structure, Immunogenicity and Glycation Properties of β-Lactoglobulin", SCIENCE-ENGINEERING (A), CHINA MASTER’S THESES FULL-TEXT DATABASE, no. 02, 15 February 2018 (2018-02-15), DOI: 20200212124913Y
TU, ZONGCAI ET AL.: "Effect of the Pretreatment Combining Glycation and Pulsed Electric Fields on the Antigenicity and Structure of β-Lactoglobulin", MODERN FOOD SCIENCE AND TECHNOLOGY, vol. 32, no. 11, 31 December 2016 (2016-12-31), DOI: 20200212125158Y
NATIONAL HEALTH AND FAMILY PLANNING COMMISSION OF PRC: "GB2760-2014 National Food Safety Standard for Uses of Food Additives", NATIONAL STANDARDS OF THE PEOPLE’S REPUBLIC OF CHINA, 24 December 2014 (2014-12-24), DOI: 20200212130315Y
YOU, XIN: "The Function and the Developing Prospect in Industry Use of Sugar Alcohols", CHINA FOOD ADDITIVES, 31 December 2010 (2010-12-31), pages 45 - 52, DOI: 20200212125828Y
KADOYA, S. ET AL.: "Freeze-Drying of Proteins with Glass-Forming Oligosaccharide-Derived Sugar Alcohols", INTERNATIONAL JOURNAL OF PARMACEUTICS, vol. 389, 25 January 2010 (2010-01-25), pages 107 - 113, XP028308326, DOI: 20200212130053A
TIAN, MING: "Effect of Pulsed Electric Fields Treatment on Structure, Immunogenicity and Glycation Properties of β-Lactoglobulin", SCIENCE-ENGINEERING (A), CHINA MASTER’S THESES FULL-TEXT DATABASE, no. 02, 15 February 2018 (2018-02-15), DOI: 20200212124913Y
TU, ZONGCAI ET AL.: "Effect of the Pretreatment Combining Glycation and Pulsed Electric Fields on the Antigenicity and Structure of β-Lactoglobulin", MODERN FOOD SCIENCE AND TECHNOLOGY, vol. 32, no. 11, 31 December 2016 (2016-12-31), DOI: 20200212125158Y
NATIONAL HEALTH AND FAMILY PLANNING COMMISSION OF PRC: "GB2760-2014 National Food Safety Standard for Uses of Food Additives", NATIONAL STANDARDS OF THE PEOPLE’S REPUBLIC OF CHINA, 24 December 2014 (2014-12-24), DOI: 20200212130315Y
YOU, XIN: "The Function and the Developing Prospect in Industry Use of Sugar Alcohols", CHINA FOOD ADDITIVES, 31 December 2010 (2010-12-31), pages 45 - 52, DOI: 20200212125828Y
KADOYA, S. ET AL.: "Freeze-Drying of Proteins with Glass-Forming Oligosaccharide-Derived Sugar Alcohols", INTERNATIONAL JOURNAL OF PARMACEUTICS, vol. 389, 25 January 2010 (2010-01-25), pages 107 - 113, XP028308326, DOI: 20200212130053A
Attorney, Agent or Firm:
HANGZHOU AOCHUANG INTELLECTUAL PROPERTY AGENCY CO., LTD (CN)
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