Title:
LAMP METHOD FOR DETECTING ERWINIA AMYLOVORA
Document Type and Number:
WIPO Patent Application WO/2012/151599
Kind Code:
A3
Abstract:
The invention relates to a method for detecting Erwinia amylovora in a sample by amplifying one or more Erwinia amylovora sequences in the sample using the LAMP method by means of primers specific to said sequence(s) and optionally comparing said sequence(s) with known sequences, characterized in that the genomic sequence of bases 378996 to 1379215 of the Erwinia amylovora genome, which codes for the hypothetical protein AMY1267 of strain Ea273, as explained in SEQ ID NO:1, is used as a reference sequence and the set of specific LAMP primers comprising SEQ ID NOs:2 to 7 is used for amplification.
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Inventors:
STICH KARL (AT)
FISCHER THILO (DE)
GOSCH CHRISTIAN (AT)
FISCHER THILO (DE)
GOSCH CHRISTIAN (AT)
Application Number:
PCT/AT2012/050060
Publication Date:
January 10, 2013
Filing Date:
May 04, 2012
Export Citation:
Assignee:
UNIV WIEN TECH (AT)
STICH KARL (AT)
FISCHER THILO (DE)
GOSCH CHRISTIAN (AT)
STICH KARL (AT)
FISCHER THILO (DE)
GOSCH CHRISTIAN (AT)
International Classes:
C12Q1/04; C12Q1/68
Foreign References:
| AT510418A4 | 2012-04-15 |
Other References:
CHRISTIAN GOSCH ET AL: "Blue EaLAMP-a specific and sensitive method for visual detection of genomic Erwinia amylovora DNA", EUROPEAN JOURNAL OF PLANT PATHOLOGY, 19 August 2012 (2012-08-19) - 19 August 2012 (2012-08-19), XP055041522, ISSN: 0929-1873, DOI: 10.1007/s10658-012-0059-5
ABOUBAKR MORADI ET AL: "Development and evaluation of a loop-mediated isothermal amplification assay for detection ofbased on chromosomal DNA", EUROPEAN JOURNAL OF PLANT PATHOLOGY, KLUWER ACADEMIC PUBLISHERS, DO, vol. 133, no. 3, 2 February 2012 (2012-02-02), pages 609 - 620, XP035060799, ISSN: 1573-8469, DOI: 10.1007/S10658-012-9939-Y
LÃ3PEZ: "Are molecular tools solving the challenges posed by detection of plant pathogenic bacteria and viruses?", CURRENT ISSUES IN MOLECULAR BIOLOGY, vol. 11, no. 1, 1 January 2009 (2009-01-01), pages 13, XP055041602, ISSN: 1467-3037
TEMPLE TODD N ET AL: "Evaluation of Loop-Mediated Isothermal Amplification for Rapid Detection of Erwinia amylovora on Pear and Apple Fruit Flowers", PLANT DISEASE, vol. 95, no. 4, April 2011 (2011-04-01), pages 423 - 430, XP002685947, ISSN: 0191-2917
GOSCH C ET AL: "New bactericides against fire blight (erwinia amylovora) and development of a fast bee-erwinia monitoring system", ACTA HORTICULTURAE, INTERNATIONAL SOCIETY FOR HORTICULTURAL SCIENCE, BE, vol. 917, 22 August 2010 (2010-08-22) - 1 January 2011 (2011-01-01), pages 139 - 142, XP009163872, ISSN: 0567-7572
GOTTSBERGER R A: "Development and evaluation of a real-time PCR assay targeting chromosomal DNA of Erwinia amylovora", LETTERS IN APPLIED MICROBIOLOGY, vol. 51, no. 3, September 2010 (2010-09-01), pages 285 - 292, XP002685948, ISSN: 0266-8254
ABOUBAKR MORADI ET AL: "Development and evaluation of a loop-mediated isothermal amplification assay for detection ofbased on chromosomal DNA", EUROPEAN JOURNAL OF PLANT PATHOLOGY, KLUWER ACADEMIC PUBLISHERS, DO, vol. 133, no. 3, 2 February 2012 (2012-02-02), pages 609 - 620, XP035060799, ISSN: 1573-8469, DOI: 10.1007/S10658-012-9939-Y
LÃ3PEZ: "Are molecular tools solving the challenges posed by detection of plant pathogenic bacteria and viruses?", CURRENT ISSUES IN MOLECULAR BIOLOGY, vol. 11, no. 1, 1 January 2009 (2009-01-01), pages 13, XP055041602, ISSN: 1467-3037
TEMPLE TODD N ET AL: "Evaluation of Loop-Mediated Isothermal Amplification for Rapid Detection of Erwinia amylovora on Pear and Apple Fruit Flowers", PLANT DISEASE, vol. 95, no. 4, April 2011 (2011-04-01), pages 423 - 430, XP002685947, ISSN: 0191-2917
GOSCH C ET AL: "New bactericides against fire blight (erwinia amylovora) and development of a fast bee-erwinia monitoring system", ACTA HORTICULTURAE, INTERNATIONAL SOCIETY FOR HORTICULTURAL SCIENCE, BE, vol. 917, 22 August 2010 (2010-08-22) - 1 January 2011 (2011-01-01), pages 139 - 142, XP009163872, ISSN: 0567-7572
GOTTSBERGER R A: "Development and evaluation of a real-time PCR assay targeting chromosomal DNA of Erwinia amylovora", LETTERS IN APPLIED MICROBIOLOGY, vol. 51, no. 3, September 2010 (2010-09-01), pages 285 - 292, XP002685948, ISSN: 0266-8254
Attorney, Agent or Firm:
ELLMEYER, Wolfgang (Mariahilfer Straße 50, Wien, AT)
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