Title:
LANTHANIDE TOOLBOX FOR ORGANELLE SPECIFIC MOLECULAR IMAGING
Document Type and Number:
WIPO Patent Application WO/2017/202379
Kind Code:
A1
Abstract:
A water soluble, simple, stable tris(N-(tert-butyl) acetamide) cyclen-based europium complex HGEU001 which exhibits the specific subcellular localization in the primary cilium with a quantum yield as high as 10% in water and a lifetime of 0.56ms lifetime. In particular, provided simplicity of the design and synthesis of a complex. Comprehensive studies were performed in numerous cell lines, such as Hela, SN-K-SH and MRC5; the motif structure, HGEU002, has also been synthesized as the negative control for in vitro imaging studies. The two photon in vitro imaging were done in three dimensions to emphasize on the specific localization in primary cilium of HGEu001. This is one of the very limited examples for direct primary cilium imaging.
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Inventors:
WONG KA LEUNG (CN)
LI HONGGUANG (CN)
CHAN CHI FAI (CN)
LAN RONGFENG (CN)
LI HONGGUANG (CN)
CHAN CHI FAI (CN)
LAN RONGFENG (CN)
Application Number:
PCT/CN2017/086101
Publication Date:
November 30, 2017
Filing Date:
May 26, 2017
Export Citation:
Assignee:
UNIV HONG KONG BAPTIST UNIV (CN)
International Classes:
C09K11/06; C07F5/00; G01N21/64
Domestic Patent References:
| WO2010084090A1 | 2010-07-29 |
Other References:
CLARKSON, IAN M. ET AL.: "Experimental assessment of the efficacy of sensitised emission in water from a europium ion, following intramolecular excitation by a phenanthridinyl group", NEW JOURNAL OF CHEMISTRY, vol. 24, no. 6, 23 May 2000 (2000-05-23), pages 377 - 386, XP055106862, Retrieved from the Internet
KIELAR FILIP ET AL.: "Two-photon microscopy study of the intracellular compartmentalization of emissive terbium complexes and their oligo-arginine and oligo-guanidinium conjugates", CHEMICAL COMMUNICATIONS, no. 21, 22 April 2008 (2008-04-22), pages 2435 - 2437, XP055448155
KIELAR FILIP ET AL.: "The nature of the sensitiser substituent determines quenching sensitivity and protein affinity and influences the design of emissive lanthanide complexes as optical probes for intracellular use", ORGANIC&BIOMOLECULAR CHEMISTRY, vol. 6, 16 May 2008 (2008-05-16), pages 2256 - 2258, XP055448158, Retrieved from the Internet
KIELAR FILIP ET AL.: "Two-photon microscopy study of the intracellular compartmentalization of emissive terbium complexes and their oligo-arginine and oligo-guanidinium conjugates", CHEMICAL COMMUNICATIONS, no. 21, 22 April 2008 (2008-04-22), pages 2435 - 2437, XP055448155
KIELAR FILIP ET AL.: "The nature of the sensitiser substituent determines quenching sensitivity and protein affinity and influences the design of emissive lanthanide complexes as optical probes for intracellular use", ORGANIC&BIOMOLECULAR CHEMISTRY, vol. 6, 16 May 2008 (2008-05-16), pages 2256 - 2258, XP055448158, Retrieved from the Internet
Attorney, Agent or Firm:
DHC IP ATTORNEYS (CN)
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