Title:
LDL RECEPTOR SIGNALING PATHWAYS
Document Type and Number:
WIPO Patent Application WO2001084159
Kind Code:
A3
Abstract:
The invention provides methods and compositions for inducing and detecting signal transduction through LDL receptors, including specifically detecting a stress that alters a functional interaction of a low density lipoprotein (LDL) receptor binding polypeptide with an LDL receptor interaction domain, by (a) introducing a predetermined stress into a system which provides a physical interaction of an LDL receptor binding polypeptide with an LDL receptor intracellular binding polypeptide interaction domain, whereby the system provides a stress-biased interaction of the binding polypeptide and the interaction domain, wherein the absence of the stress, the system provides a unbiased interaction of the binding polypeptide and the interaction domain; and (b) detecting the stress-biased interaction of the binding polypeptide and the interaction domain, wherein the binding polypeptide is selected from SEMCAP-1, JIP-1, PSD-95, JIP-2, Talin, OMP25, CAPON, PIP4,5 kinase, Na channel brain 3, Mint1, ICAP-1 and APC subunit 10.
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Inventors:
HERZ JOACHIM
GOTTHARDT MICHAEL
GOTTHARDT MICHAEL
Application Number:
PCT/US2001/013214
Publication Date:
January 16, 2003
Filing Date:
April 24, 2001
Export Citation:
Assignee:
UNIV TEXAS (US)
International Classes:
G01N33/92; (IPC1-7): G01N33/566; G01N33/92; C07K14/705; C07K14/775
Domestic Patent References:
| WO1997033173A1 | 1997-09-12 |
Foreign References:
| US6043083A | 2000-03-28 |
Other References:
TROMMSDORFF M ET AL: "Interaction of cytosolic adaptor proteins with neuronal apolipoprotein E receptors and the amyloid precursor protein", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 273, no. 50, 11 December 1998 (1998-12-11), pages 33556 - 33560, XP002178637, ISSN: 0021-9258
HERZ JOACHIM ET AL: "Cellular signalling by lipoprotein receptors.", CURRENT OPINION IN LIPIDOLOGY, vol. 11, no. 2, April 2000 (2000-04-01), April, 2000, pages 161 - 166, XP001097870, ISSN: 0957-9672
GOTTHARDT MICHAEL ET AL: "Interactions of the low density lipoprotein receptor gene family with cytosolic adaptor and scaffold proteins suggest diverse biological functions in cellular communication and signal transduction.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 275, no. 33, 18 August 2000 (2000-08-18), pages 25616 - 25624, XP002209674, ISSN: 0021-9258
DE VRIES LUC ET AL: "GIPC, a PDZ domain containing protein, interacts specifically with the C terminus of RGS-GAIP.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 95, no. 21, 13 October 1998 (1998-10-13), Oct. 13, 1998, pages 12340 - 12345, XP002209675, ISSN: 0027-8424
NEMOTO Y ET AL: "RECRUITMENT OF AN ALTERNATIVELY SPLICED FORM OF SYNAPTOJANIN 2 TO MITOCHONDRIA BY THE INTERACTION WITH THE PDZ DOMAIN OF A MITOCHONDRIAL OUTER MEMBRANE PROTEIN", EMBO JOURNAL, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 18, no. 11, 1999, pages 2991 - 3006, XP002934269, ISSN: 0261-4189
JAFFREY S R ET AL: "CAPON: a protein associated with neuronal nitric oxide synthase that regulates its interaction with PSD95", NEURON, CAMBRIDGE, MA, US, vol. 20, no. 1, 23 January 1998 (1998-01-23), pages 115 - 124, XP002104610
HERZ JOACHIM ET AL: "Cellular signalling by lipoprotein receptors.", CURRENT OPINION IN LIPIDOLOGY, vol. 11, no. 2, April 2000 (2000-04-01), April, 2000, pages 161 - 166, XP001097870, ISSN: 0957-9672
GOTTHARDT MICHAEL ET AL: "Interactions of the low density lipoprotein receptor gene family with cytosolic adaptor and scaffold proteins suggest diverse biological functions in cellular communication and signal transduction.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 275, no. 33, 18 August 2000 (2000-08-18), pages 25616 - 25624, XP002209674, ISSN: 0021-9258
DE VRIES LUC ET AL: "GIPC, a PDZ domain containing protein, interacts specifically with the C terminus of RGS-GAIP.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 95, no. 21, 13 October 1998 (1998-10-13), Oct. 13, 1998, pages 12340 - 12345, XP002209675, ISSN: 0027-8424
NEMOTO Y ET AL: "RECRUITMENT OF AN ALTERNATIVELY SPLICED FORM OF SYNAPTOJANIN 2 TO MITOCHONDRIA BY THE INTERACTION WITH THE PDZ DOMAIN OF A MITOCHONDRIAL OUTER MEMBRANE PROTEIN", EMBO JOURNAL, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 18, no. 11, 1999, pages 2991 - 3006, XP002934269, ISSN: 0261-4189
JAFFREY S R ET AL: "CAPON: a protein associated with neuronal nitric oxide synthase that regulates its interaction with PSD95", NEURON, CAMBRIDGE, MA, US, vol. 20, no. 1, 23 January 1998 (1998-01-23), pages 115 - 124, XP002104610
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