Title:
LIBRARY CONSTRUCTION METHOD BASED ON LONG OVERHANG SEQUENCE LIGATION
Document Type and Number:
WIPO Patent Application WO/2022/267843
Kind Code:
A1
Abstract:
Provided is a library construction method based on long overhang sequence ligation, a library constructed by the library construction method, for example, a CRISPR library of pair-specific multiplexed guide RNA (gRNA) combinations, and a use of the library. The CRISPR library of pair-specific multiplexed gRNA combinations can be used to simultaneously perturb multiple (e.g., 4) pre-designed targets in CRISPR/Cas9 screening. The CRISPR library of pair-specific multiplexed gRNA combinations can be a powerful tool for studying the combinatorial outcomes from coordinated gene behaviors.
Inventors:
LU ZHIKE (CN)
MA LIJIA (CN)
MA LIJIA (CN)
Application Number:
PCT/CN2022/096250
Publication Date:
December 29, 2022
Filing Date:
May 31, 2022
Export Citation:
Assignee:
UNIV WESTLAKE (CN)
International Classes:
C12N15/63; A61K35/761; C12N9/16; C12N9/22; C12N15/861
Foreign References:
| US20190330616A1 | 2019-10-31 | |||
| US20160264981A1 | 2016-09-15 | |||
| US20170175110A1 | 2017-06-22 | |||
| CN106164271A | 2016-11-23 | |||
| CN107636163A | 2018-01-26 |
Other References:
ZHOU PENG, CHAN BECKY K.C., WAN YUK KEI, YUEN CHAYA T.L., CHOI GIGI C.G., LI XINRAN, TONG CINDY S.W., ZHONG SOPHIA S.W., SUN JIERA: "A Three-Way Combinatorial CRISPR Screen for Analyzing Interactions among Druggable Targets", CELL REPORTS, ELSEVIER INC, US, vol. 32, no. 6, 1 August 2020 (2020-08-01), US , pages 108020, XP093016583, ISSN: 2211-1247, DOI: 10.1016/j.celrep.2020.108020
Attorney, Agent or Firm:
CCPIT PATENT AND TRADEMARK LAW OFFICE (CN)
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